Development and Validation of a Stability-Indicating HPLC Method for the Determination of Pizotifen, Methylparaben, and Propylparaben in Syrup

Louhaichi, Mohamed Radhouan; Jebali, Sami; Kallel, M.; Adhoum, Nafaâ; Benhamida, Nejib; Monser, Lotfi

doi:10.1080/10826076.2013.765454

Cited by 3 publications

(2 citation statements)

References 15 publications

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“…The nature of the stationary phase was chosen on the basis of previous works [4, 32, 41]. The majority of the published HPLC method uses octadecylsilica columns (C18) for the separation of antimigraine drugs.…”

Section: Resultsmentioning

confidence: 99%

“…In order to quantify the influence of operating parameters on the simultaneous separation of antimigraine drugs, four main factors were chosen: % of ACN in the mobile phase ( U 1 ), mobile-phase pH ( U 2 ), nature of the buffer ( U 3 ), and column oven temperature ( U 4 ). These variables with their respective domain are chosen on the basis of the previous work [41] and preliminary studies, and they are presented in Table 1. Experiments were performed on a two-level full factorial design 2 k [42, 43].…”

Section: Resultsmentioning

confidence: 99%

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Application of Factorial and Doehlert Designs for the Optimization of the Simultaneous Separation and Determination of Antimigraine Drugs in Pharmaceutical Formulations by RP-HPLC-UV

Jebali

Belgacem

Louhaichi

et al. 2019

International Journal of Analytical Chemistry

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A sensitive, precise, accurate, and specific isocratic reversed-phase high-performance liquid chromatographic (RP-HPLC) method for the simultaneous separation and determination of zolmitriptan, naratriptan, dihydroergotamine, ketotifen, and pizotifen in pharmaceutical formulations has been developed and validated. An experimental design was applied for the optimization of the chromatographic parameters. A two-level full factorial 2k was used for studying the interaction between the variables to be optimized: the percentage of acetonitrile in the mobile phase, mobile-phase pH, nature of the buffer, and column oven temperature. The most significant parameters are the percentage of acetonitrile and the mobile-phase pH. These significant parameters were optimized using the Doehlert matrix. The optimum separation was achieved by means of a Waters XBridge C18 column (250 mm × 4.6 mm, 5 μm) with a mobile phase consisting of acetonitrile and a 10 mM sodium perchlorate buffer (38 : 62, v/v) at a flow rate of 1.0 mL·min−1 and UV detection at 220 nm. The selectivity, method linearity, accuracy, and precision were examined as part of the method validation. The described method shows excellent linearity over a range of 30 to 70 μg·mL−1 for all compounds with correlation coefficients higher than 0.995. The standard deviations of the intraday and interday precision were between 0.75 and 1.94%. The validated method was successfully applied to perform routine analysis of these compounds in different pharmaceutical products such as syrups and tablets. In the presence of some preservatives, it was found that there were no peaks at the related peak locations.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Application of Factorial and Doehlert Designs for the Optimization of the Simultaneous Separation and Determination of Antimigraine Drugs in Pharmaceutical Formulations by RP-HPLC-UV

Jebali

Belgacem

Louhaichi

et al. 2019

International Journal of Analytical Chemistry

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Sustainable, Green, and Sensitive Spectrofluorimetric Approach for Pizotifen Maleate Detection in Tablets and Human Plasma via Photo‐Induced Electron Transfer Inhibition

Omar,

Mohamed,

Alahmadi

et al. 2024

Luminescence

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Pizotifen maleate (PZM) is an anti‐migraine drug. Effective treatment requires accurate measurement of PZM levels in patients and constant medication concentrations in tablets. This paper presents a new spectrofluorimetric approach for detecting PZM in tablets and human blood plasma (spiked with PZM). The detection mechanism depends on the inhibition of the PZM molecule's photoinduced electron transfer (PET), resulting in a bright fluorescent intensity from PZM. Acetic acid (2.0 M) acidifies the reaction environment and causes PET inhibition. This approach detects PZM at concentrations ranging from 100 to 700 ng mL−1. Furthermore, the approach has outstanding detection and quantification limits of 30.95 and 93.78 ng mL−1, respectively. Also, the approach accurately measured PZM content homogeneity in each tablet and spiked human plasma samples without any interference. This new strategy represents a new and sensitive method for accurate PZM monitoring in different samples. Furthermore, the established fluorimetric method's greenness was evaluated.

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Determination of Commonly Used Excipients in Pharmaceutical Preparations by Microchip Electrophoresis with Conductivity Detection

Troška

Poboży

Némethová³

et al. 2019

Chromatographia

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Development and Validation of a Stability-Indicating HPLC Method for the Determination of Pizotifen, Methylparaben, and Propylparaben in Syrup

Cited by 3 publications

References 15 publications

Application of Factorial and Doehlert Designs for the Optimization of the Simultaneous Separation and Determination of Antimigraine Drugs in Pharmaceutical Formulations by RP-HPLC-UV

Application of Factorial and Doehlert Designs for the Optimization of the Simultaneous Separation and Determination of Antimigraine Drugs in Pharmaceutical Formulations by RP-HPLC-UV

Sustainable, Green, and Sensitive Spectrofluorimetric Approach for Pizotifen Maleate Detection in Tablets and Human Plasma via Photo‐Induced Electron Transfer Inhibition

Determination of Commonly Used Excipients in Pharmaceutical Preparations by Microchip Electrophoresis with Conductivity Detection

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