Development and validation of a modified QuEChERS method coupled with LC-MS/MS to determine arbutin in pear peels

Kim, Jueun; Kim, Jae Il; Lee, Chul Won

doi:10.1007/s10068-016-0160-1

Cited by 10 publications

(4 citation statements)

References 31 publications

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“…Next, the developed LC-MS method based on [d 4 ]-arbutin as an IS was used to detect arbutin in Brigitta (0.43 ± 0.01 lg/mg of dry weight, DW) and Duke (0.40 ± 0.01 lg/mg of DW) blueberry leaves, as well as strawberry leaves (0.16 ± 0.02 lg/mg of fresh weight, FW) and pear peels (6.86 ± 1.39 lg/mg of DW). The results from these measurements were in agreement with previous studies, which report the concentrations of arbutin in blueberry leaves and pear peels to be 0.35 ± 0.01 lg/ mg of DW and in the range of 0.92-27.70 lg/mg of DW, respectively [17,35,36]. To the best of our knowledge, the concentration of arbutin in strawberry leaves (Fragaria spp.)…”

Section: Methods Validation Using Lc-mssupporting

confidence: 92%

Determination and quantification of arbutin in plants using stable isotope dilution liquid chromatography–mass spectrometry

Kim

Park

et al. 2018

Appl Biol Chem

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Arbutin is a very safe whitening agent for human skin. Since it is more expensive than other agents and has a challenging synthesis, novel methods to obtain this valuable agent are needed. In this study, we developed a precise and accurate method to detect and quantify arbutin using stable isotope dilution liquid chromatography-mass spectrometry (LC-MS). One challenge that needed to be overcome was the matrix effect occurring during the LC-MS analysis due to the analyte ionisation enhancement or suppression in the electrospray ionisation source by coeluting compounds. Notably, arbutin had different matrix effects in the various sample matrices. A solution to this problem was the use of [d 4 ]-arbutin as a stable isotopelabelled internal standard (SIL-IS), as it compensated the matrix effect of arbutin because it was affected by almost the same matrix effect. The validation of the developed method showed excellent linearity (r 2 = 1.000), precision (relative standard deviation B 2.5%), accuracy (recovery, 97.42-98.52%), limit of detection (0.03 lg/mL), and limit of quantification (0.1 lg/mL). Finally, the method of arbutin detection was applied to blueberry leaves to compare the precision and accuracy results obtained by performing stable isotope dilution using LC-MS and gas chromatography-mass spectrometry. The method was applied to strawberry leaves and pear peels, indicating that the SIL-IS method can be expected to find application in the arbutin analysis in other plants.

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Section: Methods Validation Using Lc-mssupporting

confidence: 92%

Determination and quantification of arbutin in plants using stable isotope dilution liquid chromatography–mass spectrometry

Kim

Park

et al. 2018

Appl Biol Chem

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“…A total of 300 peaks were detected in the chromatogram. By comparing the m/z values, the RTand the fragmentation patterns with the MS 2 spectral data taken from the literature [2,17,[35][36][37][38][39][40][41][42][43][44][45][46][47][48][49][50] or to search the data bases (MS2T, MassBank, HMDB). 78 metabolites were putatively identified as phenols, aromatic compounds, phenyl alkanoids, flavonoids, monoterpenoids, acyclic alcohol glycosides, anthocyanins etc.…”

Section: Resultsmentioning

confidence: 99%

Simultaneous Determination of 78 Compounds of Rhodiola rosea Extract by Supercritical CO2-Extraction and HPLC-ESI-MS/MS Spectrometry

Zakharenko

Razgonova

Pikula

et al. 2021

Biochemistry Research International

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The plant Rhodiola rosea L. of family Crassulaceae was extracted using the supercritical CO2-extraction method. Several experimental conditions were investigated in the pressure range of 200–500 bar, with the used volume of cosolvent ethanol in the amount of 1% in the liquid phase at a temperature in the range of 31–70°C. The most effective extraction conditions are pressure 350 bar and temperature 60°C. The extracts were analyzed by HPLC with MS/MS identification. 78 target analytes were isolated from Rhodiola rosea (Russia) using a series of column chromatography and mass spectrometry experiments. The results of the analysis showed a spectrum of the main active ingredients Rh. rosea: salidroside, rhodiolosides (B and C), rhodiosin, luteolin, catechin, quercetin, quercitrin, herbacetin, sacranoside A, vimalin, and others. In addition to the reported metabolites, 29 metabolites were newly annotated in Rh. rosea. There were flavonols: dihydroquercetin, acacetin, mearnsetin, and taxifolin-O-pentoside; flavones: apigenin-O-hexoside derivative, tricetin trimethyl ether 7-O-hexosyl-hexoside, tricin 7-O-glucoronyl-O-hexoside, tricin O-pentoside, and tricin-O-dihexoside; flavanones: eriodictyol-7-O-glucoside; flavan-3-ols: gallocatechin, hydroxycinnamic acid caffeoylmalic acid, and di-O-caffeoylquinic acid; coumarins: esculetin; esculin: fraxin; and lignans: hinokinin, pinoresinol, L-ascorbic acid, glucaric acid, palmitic acid, and linolenic acid. The results of supercritical CO2-extraction from roots and rhizomes of Rh. rosea, in particular, indicate that the extract contained all biologically active components of the plant, as well as inert mixtures of extracted compositions.

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“…Exposures to DEHP, BBP, DBP, and DEP are associated with dermatitis, conjunctivitis, and allergic symptoms [47]. Other studies have found a relationship of asthma and worsening of pulmonary functions with these phthalates [48].…”

Section: Quantification Of Phthalates In Plastic-based Food Packagingmentioning

confidence: 99%

Evaluation of the Occurrence of Phthalates in Plastic Materials Used in Food Packaging

et al. 2021

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Phthalates are multifunctional synthetic chemicals found in a wide array of consumer and industrial products, mainly used to improve the mechanical properties of plastics, giving them flexibility and softness. In the European Union, phthalates are prohibited at levels greater than 0.1% by weight in most food packaging. In the current study, headspace solid-phase microextraction (HS-SPME) combined with gas chromatography-mass spectrometry (GC-MS) was optimized, through the multivariate optimization process, and validated to evaluate the occurrence of four common phthalates, di-iso-butyl phthalate (DIBP), butyl-benzyl phthalate (BBP), di-n-octyl phthalate (DOP), and 2,2,4,4-tetrabromodiphenyl (BDE), in different food packaging. The best extraction efficiency was achieved using the polydimethylsiloxane/divinylbenzene (PDMS/DVB) fiber at 80 °C for 30 min. The validated method showed good linearity, precision (RSD < 13%), and recoveries (90.2 to 111%). The limit of detection (LOD) and of quantification (LOQ) ranged from 0.03 to 0.08 µg/L and from 0.10 to 0.24 µg/L, respectively. On average, the phthalates concentration varied largely among the assayed food packaging. DIBP was the most predominant phthalate in terms of occurrence (71.4% of analyzed simples) and concentration (from 3.61 to 10.7 μg/L). BBP was quantified in only one sample and BDE was detected in trace amounts (<LOQ) in only two samples.

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Development and validation of a modified QuEChERS method coupled with LC-MS/MS to determine arbutin in pear peels

Cited by 10 publications

References 31 publications

Determination and quantification of arbutin in plants using stable isotope dilution liquid chromatography–mass spectrometry

Determination and quantification of arbutin in plants using stable isotope dilution liquid chromatography–mass spectrometry

Simultaneous Determination of 78 Compounds of Rhodiola rosea Extract by Supercritical CO2-Extraction and HPLC-ESI-MS/MS Spectrometry

Evaluation of the Occurrence of Phthalates in Plastic Materials Used in Food Packaging

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