Development and validation of a capillary electrophoresis method for direct measurement of isocitric, citric, tartaric and malic acids as adulteration markers in orange juice

Saavedra, Luis Celi; Garcı́a, Antonia; Barbas, Coral

doi:10.1016/s0021-9673(00)00258-2

Cited by 82 publications

(56 citation statements)

References 18 publications

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“…In some cases phosphate buffer was used without any additives [21,23,25]. Following the recommendation from the literature an initial optimization of separation conditions was started with a 20 mM BGE.…”

Section: Optimization Of the Bgementioning

confidence: 99%

“…A high alkaline pH was used in these applications; hence, a BGE of 20 mM ammonium acetate, pH 9.5, was examined in this work. Several methods reported for CE determination of carboxylic acid and anionic surfactants with indirect UV detection utilized naphthalene sulfonates as BGE [22,23], hence 2 mM naphthalenedisulfate (NDS), pH 9.5, as BGE was also examined.…”

Section: Optimization Of the Bgementioning

confidence: 99%

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Separation of perfluorocarboxylic acids using capillary electrophoresis with UV detection

et al. 2005

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A capillary electrophoretic method with UV detection for separation and quantitation of perfluorocarboxylic acids (PFCAs) from C6-PFCA to C12-PFCA has been developed. The optimization of measurement conditions included the choice of the most appropriate type and concentration of buffer in the background electrolyte (BGE), as well as the type and the content of an organic modifier. The optimal separation of investigated PFCAs was achieved with 50 mM phosphate buffer and 40% isopropanol in the BGE using direct UV detection. The optimum wavelength for direct UV detection was optimized at 190 nm. For indirect detection, several chromophores were studied. Five mM 3,5-Dinitrobenzoic acid (3,5-DNBA) in 20 mM phosphate buffer BGE and indirect UV detection at 280 nm gave the optimal detection and separation performance for the investigated PFCAs. The possibility of on-line preconcentration of solutes by stacking has been examined for indirect detection. The detection limits (LODs) determined for direct UV detection ranged from 2 microg/mL for C6-PFCA to 33 microg/mL for C12-PFCA. The LODs obtained for indirect UV detection were comparable to those obtained for direct UV detection.

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Section: Optimization Of the Bgementioning

confidence: 99%

Section: Optimization Of the Bgementioning

confidence: 99%

Separation of perfluorocarboxylic acids using capillary electrophoresis with UV detection

et al. 2005

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“…The chemical compositions of juices from one specific chemical class (i.e., sugars, flavonoids, organic acids, etc.) could be determined for comparison and identification purposes (Saavedra et al 2000). However, they often suffer from some disadvantages, namely time-consuming, expensive, and requiring significant expertise or skilled personnel, which limit their applications to rapid detection and process control (Huang et al 2015).…”

mentioning

confidence: 99%

Detection of adulteration in freshly squeezed orange juice by electronic nose and infrared spectroscopy

Shen¹,

Wu²,

Su³

et al. 2016

Czech J. Food Sci.

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Abstract:Shen F., Wu Q., Su A., Tang P., Shao X., Liu B. (2016): Detection of adulteration in freshly squeezed orange juice by electronic nose and infrared spectroscopy. Czech J. Food Sci., 34: 224-232.The use of electronic nose and attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR) as rapid tools for detection of orange juice adulteration has been preliminarily investigated and compared. Freshly squeezed orange juices were tentatively adulterated with 100% concentrated orange juices at levels ranging from 0% to 30% (v/v). Then the E-nose response signals and FTIR spectra collected from samples were subjected to multivariate analysis by principal component analysis (PCA) and linear discriminant analysis (LDA). PCA indicated that authentic juices and adulterated ones could be approximately separated. For the classification of samples with different adulteration levels, the overall accuracy obtained by LDA in prediction was 91.7 and 87.5% for E-nose and ATR-FTIR, respectively. Gas chromatography-mass spectrometry (GC-MS) results verified that there existed an obvious holistic difference in flavour characteristics between fresh squeezed and concentrated juices. These results demonstrated that both E-nose and FTIR might be used as rapid screening techniques for the detection of this type of juice adulteration.

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“…Compared with other CE/CEC methods on the separation and determination of organic acids, this method had some obvious advantages, such as fast and efficient separation, no EOF modifiers (e.g., organic reagents and surfactants) used in the analysis process, no timeconsuming column fabrication and no severe detection interference etc. (Flottmann et al 2004;Li et al 2013;Kuban and Karlberg 1997;Saavedra et al 2000;Zhu et al 2012). To the best of our knowledge, this was the first report on the separation and detection of organic acids in real blueberry juice samples by using hexadimethrine bromide as PSPs in CE-ESIMS.…”

Section: Introductionmentioning

confidence: 86%

Simultaneous separation and determination of organic acids in blueberry juices by capillary electrophoresis- electrospray ionization mass spectrometry

Yong-ku

Wang

et al. 2014

J Food Sci Technol

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A sensitive and expeditious capillary e l e c t r o p h o r e s i s -e l e c t r o s p r a y i o n i z a t i o n m a s s spectrometry(CE-ESIMS) method for the separation, identification and determination of succinic, citric, salicylic, malic, benzoic, sorbic, ascorbic, and tartaric acid in blueberry juices has been developed. In order to obtain the analytical separation, CE-MS interface parameters(e.g., buffer pH and composition, sheath liquid and gas flow rates, sheath liquid composition, electrospray voltage, etc.) were carefully optimized. Eight organic acids were baseline separated in 8 min under optimum experimental conditions. The precisions for eight replicate separations of a standard mixture solution were 2.54-4.12 % for peak area and 0.85-2.12 % for migration time respectively. The linear ranges were 10.0-1000.0, 8.0-1000.0, 13.0-1000.0, 6.0-1000.0, 9.0-1000.0, 3.2-600.0, 6.0-1000.0 and 18.0-1000.0 μg/L for succinic, citric, salicylic, malic, benzoic, sorbic, ascorbic, and tartaric acid with detection limits of 2.5, 2.0, 3.4, 1.5, 2.2, 0.8, 1.5, 4.5 μg/L, respectively. The average recoveries of the eight components were between 86.8 and 99.8 % with RSDs of 1.8-5.3 %. The proposed method was applied to the simultaneous analysis of eight analytes in Blueberry Juice samples with satisfactory results.

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Development and validation of a capillary electrophoresis method for direct measurement of isocitric, citric, tartaric and malic acids as adulteration markers in orange juice

Cited by 82 publications

References 18 publications

Separation of perfluorocarboxylic acids using capillary electrophoresis with UV detection

Separation of perfluorocarboxylic acids using capillary electrophoresis with UV detection

Detection of adulteration in freshly squeezed orange juice by electronic nose and infrared spectroscopy

Simultaneous separation and determination of organic acids in blueberry juices by capillary electrophoresis- electrospray ionization mass spectrometry

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