Development and validation of a gene expression oligo microarray for the gilthead sea bream (Sparus aurata)

Abstract

Background: Aquaculture represents the most sustainable alternative of seafood supply to substitute for the declining marine fisheries, but severe production bottlenecks remain to be solved. The application of genomic technologies offers much promise to rapidly increase our knowledge on biological processes in farmed species and overcome such bottlenecks. Here we present an integrated platform for mRNA expression profiling in the gilthead sea bream (Sparus aurata), a marine teleost of great importance for aquaculture.

Transcriptomic Characterization of the Larval Stage in Gilthead Seabream (Sparus aurata) by 454 Pyrosequencing

“…To date, only limited genomic and transcriptomic studies on fish embryos and yolksac larvae have been conducted in a few teleost fish species: in zebrafish, Danio rerio, (Packham et al 2009;Ton et al 2002), in gilthead seabream (Ferrareso et al 2008;Sarropoulou et al 2005b), in cod (Drivenes et al 2012;Johansen et al 2011) and in the marine killifish, Fundulus heteroclitus, (Bozinovic et al 2011). To our knowledge, only the EST collections generated for halibut (Douglas et al 2007) and…”

Molecular profiling of the gilthead sea bream (Sparus aurata L.) response to chronic exposure to the myxosporean parasite Enteromyxum leei

“…For gilthead sea bream, microarrays have been constructed to study early development and response to cortisol injection (Sarropoulou et al, 2005), other developmental processes (Ferraresso et al, 2008) and the effect of confinement exposure (Calduch-Giner et al, 2010).…”

Population Genomics of Marine Fishes: Next-Generation Prospects and Challenges

“…Therefore, it is not surprising that RNA-seq has become the technology of choice for quantifying differential gene expression (Deng et al, 2011;Smith et al, 2013). Gene expression analyses have allowed the identification of key molecular mechanisms underlying desired traits in farmed fish (Roberge et al, 2006;Ferraresso et al, 2008Ferraresso et al, , 2013, but have also been successfully applied to natural populations of marine fishes (Whitehead and Crawford, 2006;Larsen et al, 2007Larsen et al, , 2011Pujolar et al, 2012Pujolar et al, , 2013bCôté et al, 2014). Finally, transcriptomic analyses can be complemented and integrated with protein expression analysis to investigate the responses at the proteomic level for a deeper understanding of functional implications (Nie et al, 2007;Dalziel and Schulte, 2012).…”