Development and validation of a green HPLC method for the analysis of clorsulon, albendazole, triclabendazole and ivermectin using monolithic column: Assessment of the greenness of the proposed method

Rashed, Noha S.; Zayed, Sahar; Abdelazeem, Amany M.; Fouad, Fatma A.

doi:10.1016/j.microc.2020.105069

Cited by 37 publications

(12 citation statements)

References 21 publications

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“…The nature of the mobile phase, especially the polarity, plays essential role in the selectivity of the chromatographic method. Recently, the choice of mobile phase is strongly recommended considering the health and environmental aspects of organic solvents 27 , 28 . The selection of mobile phase was based on the ranking of solvents in terms of green analytical chemistry metrics 29 .…”

Section: Resultsmentioning

confidence: 99%

Adjusted green HPLC determination of nirmatrelvir and ritonavir in the new FDA approved co-packaged pharmaceutical dosage using supported computational calculations

Imam

Batubara

Gamal

et al. 2023

Sci Rep

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The greening of analytical methods has gained interest in the quantitative analysis field to reduce environmental impact and improve safety health conditions for analysts. Nirmatrelvir plus ritonavir is a new FDA approved co-packaged medication developed for the treatment of COVID-19. The aim of this research was to develop green fitted HPLC method using pre experimental computational testing of different stationary phases as well as selecting mobile phase regarding to green analytical chemistry principles. Computational study was designed to test the physical interaction between nirmatrelvir and ritonavir and different columns (C8, C18, Cyano column). The study showed that the C18 column was better for simultaneous HPLC analysis of the cited drugs. Regarding to green point of view, mobile phase consisted of ethanol: water (80:20, v/v) provided an efficient chromatographic separation of nirmatrelvir and ritonavir within a short analytical run time, reasonable resolution and excellent sensitivity. Isocratic elution was performed on a selected C18 column and a green adjusted mobile phase at flow rate of 1 mL/min and UV detection at 215 nm. The chromatographic system allowed complete baseline separation with retention times of 4.9 min for nirmatrelvir and 6.8 min for ritonavir. The method succeeded to determine nirmatrelvir and ritonavir over the concentration range of 1.0–20.0 μg/mL in the pure form and in pharmaceutical dosage form. Greenness profiles of the applied HPLC method was assessed using analytical eco-scale, the green analytical procedure index and the AGREE evaluation method. The results revealed adherence of the described method to the green analytical chemistry principles. The authors hope to provide a promising challenge for achieving green goals through integrating computational tools and applying them with green assessment metrics.

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Section: Resultsmentioning

confidence: 99%

Adjusted green HPLC determination of nirmatrelvir and ritonavir in the new FDA approved co-packaged pharmaceutical dosage using supported computational calculations

Imam

Batubara

Gamal

et al. 2023

Sci Rep

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show abstract

“…Significant contributions in the field of GAC metrics were made by several experts in 2006 and 2012, which is highly essential to measure the overall adverse impact of various analytical procedures [9,10]. The one proposed in 2012 is a more comprehensive semi‐quantitative approach and is adopted for multiple field applications due to its superiority over other available tools [2,5,11]. However, sustainable green method performance can be obtained if AES metrics are implemented at the beginning of method development rather than on an already developed and validated analytical method [12].…”

Section: Introductionmentioning

confidence: 99%

Development of a validated liquid chromatography–diode array detection method for simultaneous determination of olanzapine and fluoxetine in their combined formulation: Application to greenness assessment

Panda

Behera

Bera

et al. 2022

Separation Science Plus

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Olanzapine and fluoxetine hydrochloride are two promising drugs recommended for use in combination to treat bipolar psychotic disorders. In the present study, a green liquid chromatographic method is reported for simultaneous quantification of the said combination by employing analytical eco‐scale principles. The penalty point scoring process revealed an excellent greener nature of the current method than the reported methods. Chromatographic separation was achieved on a C‐18 column employing methanol and phosphate buffer of pH 3.5 as the mobile phase at a flow rate of 1.0 mL/min and detected at 227 nm. Both the analytes were separated with resolution > 2.0. Method validation revealed apt linearity (r2 = 0.999), accuracy (> 99%), and precision (overall % relative standard deviation < 1%) for quantification of the analytes. The limits of detection and quantitation were compliant as per study intent. Further, a systematized chemometrics approach using risk assessment and design of experiments revealed the influence of critical method variables on the method's robustness. Optimum system suitability and assay results confirmed selectivity and revealed the specific nature of the method. Overall, a robust and greener analytical method is established for the routine simultaneous quantification of the two antipsychotics in their combined tablet dosage form.

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“…Many chromatographic methods have been developed for IVM determination in different matrices [5][6][7][8]; however, there is only two electroanalytical method for its analysis reported in the literature to our knowledge. According to the works developed by Lourenção et al [9] and Mahnashi et al [10], the IVM molecule presented an oxidation process at approximately + 1.4 V and + 0.95 V vs. Ag/AgCl, respectively.…”

Section: Introductionmentioning

confidence: 99%

Competitive Host‐guest Electrochemical Detection of Ivermectin Drug Using a β‐Cyclodextrin/Graphene‐based Electrode

et al. 2022

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The present study describes the novel development and application of an ivermectin (IVM) sensing electrochemical platform based on reduced graphene oxide (rGO) and the macrocyclic host β-cyclodextrin (β-CD) molecule. The sensing method was based in the hostguest characteristics of β-CD and competitive interaction between the target analyte and the methylene blue (MB) redox probe. Differential pulse voltammetry (DPV) was employed for the detection of IVM and a linear response between 0.5 and 40.0 μmol L À 1 with a limit of detection of 0.25 μmol L À 1 was obtained using the glassy carbon (GC)/ rGO/β-CD electrode. The sensing platform was successfully applied for the detection of IVM in tap water samples, which may expand the applications of β-CD towards the analysis of other chemical species.

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Development and validation of a green HPLC method for the analysis of clorsulon, albendazole, triclabendazole and ivermectin using monolithic column: Assessment of the greenness of the proposed method

Cited by 37 publications

References 21 publications

Adjusted green HPLC determination of nirmatrelvir and ritonavir in the new FDA approved co-packaged pharmaceutical dosage using supported computational calculations

Adjusted green HPLC determination of nirmatrelvir and ritonavir in the new FDA approved co-packaged pharmaceutical dosage using supported computational calculations

Development of a validated liquid chromatography–diode array detection method for simultaneous determination of olanzapine and fluoxetine in their combined formulation: Application to greenness assessment

Competitive Host‐guest Electrochemical Detection of Ivermectin Drug Using a β‐Cyclodextrin/Graphene‐based Electrode

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