Development and validation of a multi-residue method for determination of 18 β-agonists in bovine urine by UPLC–MS/MS

Mauro, D.; Ciardullo, Silvia; Civitareale, Cinzia; Fiori, Maurizio; Pastorelli, Augusto Alberto; Stacchini, Paolo; Palleschi, Giuseppe

doi:10.1016/j.microc.2014.02.012

Cited by 32 publications

(18 citation statements)

References 35 publications

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“…The analytical methods hitherto used for the detection of ˇ2-agonists include hapten microarray [9], capillary electrophoresis (CE) [10,11], gas chromatography mass spectrometry (GC-MS) [12] and liquid chromatography-mass spectrometry (LC-MS) [13][14][15][16][17][18]. However, there are limitations with these methods.…”

mentioning

confidence: 99%

Ultra-trace analysis of 12 β2-agonists in pork, beef, mutton and chicken by ultrahigh-performance liquid-chromatography–quadrupole-orbitrap tandem mass spectrometry

Guo

Shi

Gong

et al. 2015

Journal of Pharmaceutical and Biomedical Analysis

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mentioning

confidence: 99%

Ultra-trace analysis of 12 β2-agonists in pork, beef, mutton and chicken by ultrahigh-performance liquid-chromatography–quadrupole-orbitrap tandem mass spectrometry

Guo

Shi

Gong

et al. 2015

Journal of Pharmaceutical and Biomedical Analysis

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“…Multi-target methods were developed respectively for 18 β 2 -agonists detection in bovine urine [11], 20 β 2 -agonists in bovine hair [12], 16 β 2 -agonists in pig liver, kidney and muscle [13], and 11 β 2 -agonists in human urine [14]. The latter method reported homogeneous LOD values of 0.1 ng/mL for all targeted analytes, including salmeterol, whose actual concentration in urine after clinical administration is frequently below this 0.1 ng/mL limit [15].…”

Section: Introductionmentioning

confidence: 99%

Development and validation of an UHPLC–MS/MS method for β 2 -agonists quantification in human urine and application to clinical samples

Bozzolino

Leporati

Gani

et al. 2018

Journal of Pharmaceutical and Biomedical Analysis

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A fast analytical method for the simultaneous detection of 24 β-agonists in human urine was developed and validated. The method covers the therapeutic drugs most commonly administered, but also potentially abused β-agonists. The procedure is based on enzymatic deconjugation with β-glucuronidase followed by SPE clean up using mixed-phase cartridges with both ion-exchange and lipophilic properties. Instrumental analysis conducted by UHPLC-MS/MS allowed high peak resolution and rapid chromatographic separation, with reduced time and costs. The method was fully validated according ISO 17025:2005 principles. The following parameters were determined for each analyte: specificity, selectivity, linearity, limit of detection, limit of quantification, precision, accuracy, matrix effect, recovery and carry-over. The method was tested on real samples obtained from patients subjected to clinical treatment under chronic or acute therapy with either formoterol, indacaterol, salbutamol, or salmeterol. The drugs were administered using pressurized metered dose inhalers. All β-agonists administered to the patients were detected in the real samples. The method proved adequate to accurately measure the concentration of these analytes in the real samples. The observed analytical data are discussed with reference to the administered dose and the duration of the therapy.

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“…At present, the β‐AA detection methods are mainly containing high performance liquid chromatography (HPLC) , magnetic solid‐phase extraction method (MSPE) , ultra‐high performance liquid chromatography (UHPLC/MS/MS) , liquid chromatography‐mass spectrometry (LC‐MS) , enzyme‐linked immunosorbent assay (ELISA) , gas chromatography‐mass spectrometry (GC‐MS) , etc. With the disadvantages of sample treatment for a long time, cumbersome detection process and difficult to operate, expensive instruments, phenomenon of false positives, the practical applications are limited.…”

Section: Introductionmentioning

confidence: 99%

Signal Amplification Strategy for Highly Sensitive Detecting Brombuterol with Electrochemiluminescent Immunoassay by Using CdSe QDs as Label and Gold Nanoparticle as Substrate

et al. 2016

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1Introduction b-adrenergic agonists (b-AA), commonlyk nowna sc lenbuterol, is akind of drugthat can significantly reduce adipose tissue accretionb ythe combinede ffects of inhibiting fatty acid biosynthesis and concurrently stimulating triacylglycerol( TAG) lipolysis,o rh ydrolysis [1].I nt his work, the detected brombuterol ( Brom, methyl] benzyl alcohol) is ak ind of b-AA [2].B rom has been used as the growth promoting agent and feed additive for cattle,s heep,p oultry and otherl ivestock on account of it significantly improved the carcass lean percentage,w eight gain and feed conversion rate [3]. b-AA residues in food from foodproducing animals may pose ar isk on human health, such as headache,m uscular pain, cardiac palpitation, dizziness, nausea, vomiting, fever and even death [4].C hina and some other countries have listed the b-AA as directory of prohibited drugs for animals.S ome illegal businessmen are optingt or educe their input costs whicha re primarily made up of feed costsi ns earch for ab etter profit margin [5,6].B rom is used as feed additive,t herefore the means for detecting residues in food and feed are badly in need of enhancement.At present, the b-AA detectionm ethodsa re mainly containing high performance liquid chromatography (HPLC) [ 7],m agnetic solid-phase extraction method (MSPE)[ 8],u ltra-highp erformance liquid chromatography (UHPLC/MS/MS) [9,10],l iquid chromatographymass spectrometry (LC-MS) [11],e nzyme-linked immu-nosorbent assay (ELISA) [ 2,12],g as chromatographymasss pectrometry (GC-MS) [13],e tc. With the disadvantages of sample treatment for al ong time,c umbersome detectionp rocess and difficult to operate,e xpensivei nstruments,p henomenon of false positives, the practical applications are limited. In this paper, electrochemical immunosensor prepared by competition immune method has been ak ind of practicalv alue and scientific value of detectingB rom.Electrochemiluminescence (ECL),a lso known as electric chemical luminescence,i sakind of analytical techniques combining the characteristics of the controllability of electrochemical potential and the high sensitivity of the luminescence analysis, which has become one of the mostp owerful analysis techniques [14][15][16].I nr ecent years,e lectrochemicall uminescence immunoassay (ECLIA) as ap owerful bioanalysis technique is composedo fE CL and immunoassayw ith values of specific identification, stability,s implified optical setup, high sen-Abstract:Asignal amplifications trategy for electrochemiluminescence (ECL)i mmunosensor was designed based on gold nanoparticles (AuNPs) and L-cysteine capped CdSe QDs for highly sensitive detection of brombuterol( Brom) in the presence of K 2 S 2 O 8 as the co-reactant. Therein, AuNPs as the substrate can simplify the experiment process, accelerate the electron transfer rate, and enhance the ECL signal. In the presence of Brom standard solution, coating antigen immobilized on the electrode surface via AuNPs would compete with Brom for the limited binding sites of the CdSeQ Ds labeled Bro...

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Development and validation of a multi-residue method for determination of 18 β-agonists in bovine urine by UPLC–MS/MS

Cited by 32 publications

References 35 publications

Ultra-trace analysis of 12 β2-agonists in pork, beef, mutton and chicken by ultrahigh-performance liquid-chromatography–quadrupole-orbitrap tandem mass spectrometry

Ultra-trace analysis of 12 β2-agonists in pork, beef, mutton and chicken by ultrahigh-performance liquid-chromatography–quadrupole-orbitrap tandem mass spectrometry

Development and validation of an UHPLC–MS/MS method for β 2 -agonists quantification in human urine and application to clinical samples

Signal Amplification Strategy for Highly Sensitive Detecting Brombuterol with Electrochemiluminescent Immunoassay by Using CdSe QDs as Label and Gold Nanoparticle as Substrate

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