Development and validation of a liquid chromatographic method for in vitro mupirocin quantification in both skin layers and percutaneous penetration studies

Echevarrı́a, Lydia; Blanco-Prieto, María J.; Campanero, Miguel Ángel; Santoyo, Susana; Ygartua, P.

doi:10.1016/j.jchromb.2003.07.011

Cited by 21 publications

(10 citation statements)

References 22 publications

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“…Spectrofluorometry has already been used for the determination of eugenol in biological tissue/fluids [37]. n Extraction recovery Eugenol recovery from the epidermis and dermis was evaluated according to the procedures described in the literature [38][39][40]. Control HCS sections of 0.64 cm 2 were exposed to heat (60°C) for epidermis separation from the rest of the skin (dermis).…”

Section: Research Article Garg and Singhmentioning

confidence: 99%

Targeting of Eugenol-Loaded Solid Lipid Nanoparticles to The Epidermal Layer of Human Skin

Garg

Singh

2014

Nanomedicine

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Section: Research Article Garg and Singhmentioning

confidence: 99%

Targeting of Eugenol-Loaded Solid Lipid Nanoparticles to The Epidermal Layer of Human Skin

Garg

Singh

2014

Nanomedicine

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“…The validation of imiquimod extraction from skin samples was performed using a procedure similar to those described in the literature (Echevarria et al, 2003;Elsayed, 2007). In general, 10 μL of imiquimod methanolic solutions corresponding to 100, 250, 500, 1000, 1500, 2000 and 2500 ng of drug were directly added to blank SC-containing adhesive tapes and tape-stripped skin samples (0.785 cm 2 surface).…”

Section: Validationmentioning

confidence: 99%

Development and validation of HPLC method for imiquimod determination in skin penetration studies

Paula

Martins

Bentley

2008

Biomedical Chromatography

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A simple, rapid and sensitive analytical procedure for the measurement of imiquimod in skin samples after in vitro penetration studies has been developed and validated. In vitro penetration studies were carried out in Franz diffusion cells with porcine skin. Tape stripping technique was used to separate the stratum corneum (SC) from the viable epidermis and dermis. Imiquimod was extracted from skin samples using a 7:3 (v/v) methanol:acetate buffer (100 mM, pH 4.0) solution and ultrasonication. Imiquimod was analyzed by HPLC using C(8) column and UV detection at 242 nm. The mobile phase used was acetonitrile:acetate buffer (pH 4.0, 100 mM):diethylamine (30:69.85:0.15, v/v) with flow rate 1 mL/min. Imiquimod eluted at 4.1 min and the running time was limited to 6.0 min. The procedure was linear across the following concentration ranges: 100-2500 ng/mL for both SC and tape-stripped skin and 20-800 ng/mL for receptor solution. Intra-day and inter-day accuracy and precision values were lower than 20% at the limit of quantitation. The recovery values ranged from 80 to 100%. The method is adequate to assay imiquimod from skin samples, enabling the determination of the cutaneous penetration profile of imiquimod by in vitro studies.

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“…Compared with this way, the quasi-emulsion solvent-diffusion method is not only easily performed in the laboratory, but also has the potential to prepare microsponges readily in the factory. 7,24 In the process of preparation, in order to optimize the preparation process of microsponges [7][8][9]25,26 and improve release of drug, 27-28 the factors including drug/polymer ratio, disperse phase solvent amount, stirring time, speed and stirrer type on the preparation of microsponges always need to be investigated. Afterwards, the thermal behavior, surface morphology, particle size and pore structure of microsponges should be examined.…”

Section: Preparation and Applicationmentioning

confidence: 99%

Toward Application of Microsponges in Transdermal Drug Delivery System

Hong¹,

Chen²,

Xiang³

et al. 2015

MOJBB

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Abbreviations: TDDS, transdermal drug delivery system; FDA, food and drug administration; EC, ethyl cellulose; BPO, benzoyl peroxide; SEM, scanning electron microscopy; AUC, area under the curve; EE, entrapment efficiency; DSC, differential scanning calorimetry; MED, minimal erythemal dose; SPF, sun protection factor IntroductionCompared with drug dosage forms for oral and parenteral administration, Transdermal drug delivery system (TDDS) can minimize and avoid some restrictions relating to dosage forms effectively. Due to the conventional delivery systems suffering from certain restrictions like Peak and Valley phenomenon i.e., they show fluctuations in plasma drug concentration and cannot render sustained effect.1 However, the TDDS can meet the requirement to keep relatively stable plasma drug concentration and prolong delivery of drug, in a steady-state profile and minimizes the peak-associated side effects, which thus ensures the level of the drug is effective for treatment on the minimal therapeutic concentration. In general, Transdermal drug delivery system, as a controlled drug delivery system, is exceedingly humanized, commodious and prolong the period of time of release of drug, if need arises (e.g. systemic toxicity) with less pain sensation while administrating drug candidates. Additionally, TDDS permits the permeation of drugs across the skin and into the systemic circulation which avoids the hepatic first-pass effect observed in the course of oral administration and inconvenience of frequent parenteral administration. As a new drug carrier of TDDS, Microsponges are mostly used for topical 4-10 and oral administration. 11-15 For other topical delivery systems, the traditional delivery systems are required of frequent dosing to keep relatively stable local concentrations of effective constituent for therapeutic effectiveness because of low effectiveness.Thus to avoid this defect it is indispensable to design a new drug delivery system to prolong the residual time of drug in the epidermis or the dermis, while reduce its penetration into the body. For this reason, as a porous polymeric microsphere, Microsponges can fulfill such aims uniquely.4 Microsponges are porous, polymeric microspheres (Figure 1) which are invariably used for topical delivery system; 4-5,7-10 its inside are full of lacunas and its particle size is 5~300μm in diameter. AbstractTransdermal drug delivery system (TDDS) is a dosage form designed to deliver drug through skin by topical route of administration to the systemic circulation or for local treatment. As a new type of dosage form, TDDS has a number of special merits, but inevitably at the same time it possess some distinct disadvantages, such as low permeability and the drug mostly enter the systemic vascular. Microsponges are a kind of micro-sized and polyporous particles which can entrap effective constituents, and are biologically safe. And Microsponges also exhibit distinctive advantage at the aspect of drug release. The TDDS can enhance drug stability, decrease side effec...

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Development and validation of a liquid chromatographic method for in vitro mupirocin quantification in both skin layers and percutaneous penetration studies

Cited by 21 publications

References 22 publications

Targeting of Eugenol-Loaded Solid Lipid Nanoparticles to The Epidermal Layer of Human Skin

Targeting of Eugenol-Loaded Solid Lipid Nanoparticles to The Epidermal Layer of Human Skin

Development and validation of HPLC method for imiquimod determination in skin penetration studies

Toward Application of Microsponges in Transdermal Drug Delivery System

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