Development and Validation of a High Performance Liquid Chromatography Method for Determination of Telmisartan in Rabbit Plasma and its Application to a Pharmacokinetic Study

Virkar, Prasad S.; Pingale, Satish G.

doi:10.4172/2155-9872.1000133

Cited by 6 publications

(3 citation statements)

References 11 publications

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“…In mice, peak plasma concentration (C max ) after one dose of 1 mg/Kg of TEL is 162 ng/mL after oral administration [7], but to date, we did not find a report for its plasma concentration when administered ad libitum to mice. Regarding methods to detect TEL, Virkar et al developed and validated an HPLC method with fluorescence detection in rabbit plasma [35]. Salama et al also reported the use of HPLC-UV in Human plasma, and was able to detect between 1-10 µg/mL of TEL [36].…”

Section: Discussionmentioning

confidence: 99%

Drug delivery to rodents: how to deal with body mass and water intake fluctuations?

Gonçalves¹,

Fernandes-Santos²

2019

Preprint

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Introduction: Animal models are used to test the safety and efficacy of drugs. They are often administered to rodents in the drinking water, but it has some limitations, such as the drug stability, variations of water consumption and body mass. We investigated telmisartan (TEL) stability in mice drinking water by UV spectrophotometry, and if water intake and body mass fluctuations change drug ingestion.Methods: Female C57BL/6 mice at two months old, were fed for eight weeks with a purified AIN93M diet, or a high-fat high-sucrose diet (HFHS). TEL 5 mg/Kg/day was administered ad libitum to mice in the drinking water during three weeks concomitant with diets, summing 11 weeks of diet feeding. Results: UV spectrophotometry could detect TEL at the wavelength of 300 nm, and it remained stable in mice drinking water for seven days, at the concentration expected. Mice gain weight after eight weeks on high-fat high-sucrose diet feeding, and TEL 5 mg/kg/day in the drinking water for three weeks reduced it. TEL did not change water intake. Not adjusting TEL concentration weekly would lead to a higher intake of TEL by mice. Discussion:We demonstrated that body mass and water intake fluctuations significantly change the amount of drug that the animal receive, and it would add a bias to the experiment. TEL remains stable for at least seven days in wrapped mice water bottles in the animal care facility, and UV spectrophotometry proved to be a simple and low-cost method to detect TEL in mice drinking water.

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Section: Discussionmentioning

confidence: 99%

Drug delivery to rodents: how to deal with body mass and water intake fluctuations?

Gonçalves¹,

Fernandes-Santos²

2019

Preprint

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“…The method provided excellent selectivity and linearity, and has been successfully applied to pharmacokinetic study. 66 Development of a HPLC method for the determination of tedizolid in human plasma, human serum, saline and mouse plasma: A simple HPLC method was developed and validated to analyze tedizolid in human plasma, human serum, saline and CD-1 mouse plasma.…”

Section: Validated Methods For the Determination Of Gemifloxacin In Bulk Pharmaceutical Formulations And Human Serum By Rp-hplcmentioning

confidence: 99%

A review of HPLC technique covering its pharmaceutical, environmental, forensic, clinical and other applications

Chawla¹,

Chaudhary²

2019

IJPCA

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HPLC is an analytical technique widely used for identification, separation, detection and quantification of various drugs and its related degradants. Appropriate mobile phase, stationary phase, column, column size, temperature, wavelength and gradient selection is important for the suitable compatibility and stability of drug as well as impurities and degradants. Most of the drugs as well as other compounds can be analyzed by HPLC method because of the several advantages like rapidity, specificity, accuracy, precision and ease of automation in this method. This article has been prepared with an aim to review different aspects of HPLC, such as principle, types, mode of separation, characteristics, instrumentation, important parameters and various applications of HPLC in different fields.

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“…4 It is also determined spectrophotometrically 9 and with TLC. [10][11][12] Telmisartan is determined in human plasma, 13,14 urine [15][16][17][18][19] and binary tablets. 20,21 It is determined by HPLC analysis using C18 column 17,18,22 with mass sensitive detector, 23,24 fluorescence,…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Determination of Sartans by High Performance Liquid Chromatography with Ultra Violet Detection

Maslarska¹,

Yankov²,

Obreshkova³

et al. 2017

IJPER

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Objective: A high performance liquid chromatographic method with ultra violet detection for simultaneous analysis of three sartans (Valsartan, Irbesartan and Telmisartan) has been developed for quality control. Method and Results: Isocratic elution on a LiChrospher C18 column (250 × 4 mm, particle size 5 µm) at the temperature 30ºC with a mobile phase consisting of 10mM phosphate buffer: acetonitrile (65:35 v/v) at a flow rate 1.0 ml/min has been done. The column eluent was monitored with a UV detector at 225 nm. This allowed a rapid detection and identification as well as quantitation of the eluting peaks. Method Validation: Calibration curves for all drugs were in the range of 5-40 µg/ml and the linear regression coefficients were more than 0.995. Recovery rates for the sartans were in the range 96.5% to 103.1%. The limits of detection were calculated between 0.04-0.06 µg/ ml. Also, the limits of quantification were 0.12-0.16 µg/ml. Within-day and between-day coefficient of variation for all sartans at all concentrations in the range of 0.83 -3.79% was calculated. Conclusion: The procedure can provide a simple, sensitive and fast method for the quality control of the three sartans in bulk and tablets.

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Development and Validation of a High Performance Liquid Chromatography Method for Determination of Telmisartan in Rabbit Plasma and its Application to a Pharmacokinetic Study

Cited by 6 publications

References 11 publications

Drug delivery to rodents: how to deal with body mass and water intake fluctuations?

Drug delivery to rodents: how to deal with body mass and water intake fluctuations?

A review of HPLC technique covering its pharmaceutical, environmental, forensic, clinical and other applications

Simultaneous Determination of Sartans by High Performance Liquid Chromatography with Ultra Violet Detection

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