Development and validation of an HPLC method for simultaneous determination of trimethoprim and sulfamethoxazole in human plasma

Sayar, Esin; Şahin, Selma; Cevheroğlu, Şemsettin; Hıncal, A. Atilla

doi:10.1007/s13318-010-0006-9

Cited by 26 publications

(11 citation statements)

References 13 publications

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“…The determinations of the concentrations of TMP/SMX were carried out using high resolution liquid chromatographic based on a method previously reported by Sayar et al [5].…”

Section: Discussionmentioning

confidence: 99%

“…Several analytical methods for the quantification of these compounds with UV detection, mass spectrometry or by electrophoresis have been developed. Previous HPLC methods have the inconvenient that the retention time is a little more prolonged or that the extraction process is more tedious or not quantified small unities [5] [6] [7] [8]. The objective of the present study was to develop a simple, economical, precise, fast and exact method of HPLC for simultaneous determination of TMP/SMX for pharmacokinetic studies.…”

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confidence: 99%

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A Reliable Method to Quantify Trimethoprim and Sulfamethoxazole in Plasma of Children by HPLC with Application in Pharmacokinetic Studies

López¹,

Olguı́n²,

Lares‐Asseff³

et al. 2019

AJAC

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Aim: To develop a simple and sensitive high-performance liquid chromatography method to determine plasmatic levels of trimethoprim/sulfamethoxazole in paediatric population. Method: Chromatographic separation was carried out using a reverse phase column with detection ultraviolet at a wavelength of 225 nm. The mobile phase consists of phosphate buffer 0.1 M, acetonitrile and methanol, (65:20:15) with a flow of 1.0 ml/min. Results: Calibration curve was linear over the concentration range for trimethoprim of 0.25 to 5 µg/ml and 5 to 100 µg/ml for sulfamethoxazole. Precision were found to be within 15% for both compounds. The detection limits and quantifications for trimethoprim/sulfamethoxazole were 0.2 -0.25 µg/ml and 3 -5 µg/ml respectively. Conclusions: The developed method was applied for the quantification of both compounds in plasma samples of two patients resulting concentrations within the therapeutic ranges. The method is convenient for pharmacokinetic studies.

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“…The determinations of the concentrations of TMP/SMX were carried out using high resolution liquid chromatographic based on a method previously reported by Sayar et al [5].…”

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

A Reliable Method to Quantify Trimethoprim and Sulfamethoxazole in Plasma of Children by HPLC with Application in Pharmacokinetic Studies

López¹,

Olguı́n²,

Lares‐Asseff³

et al. 2019

AJAC

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“…The optimal ratio of the two agents for their synergistic activity has been found to be 5:1. (3) Various methods have been reported for the simultaneous determination of the combination of SMX and TMP in pharmaceutical formulations and biological fluids, such as (HPLC) (4)(5)(6) , Thin layer chromatography (TLC) (7) , capillary zone electrophoresis method (8) , Voltametric methods (9)(10) , Spectrophotometric methods (11)(12)(13) , and Derivative spectrophotometric methods (14)(15)(16) .…”

Section: Sulphamethoxazolementioning

confidence: 99%

Simultaneous Determination of Sulphamethoxazole and Trimethoprim in Binary Mixtures and in Tablet Using Derivative Spectrophotometry

Al-Saidi¹,

Yonis²

2015

JNUS

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Derivative spectrophotometric (first, second, third and fourth derivative) were developed for the determination of Sulphamethoxazole)SMX) and trimethoprim (TMP)antibiotic binary mixture by applying zero-crossing technique for pure synthetic mixture while simultaneous determination of (SMX) and (TMP) in tabletsusing D 4 at waveleanth 257.8 nm and 251.5 nm for (SMX) and (TMP) the correlation coefficient of calibration curves were 0.9992 and 0.9995. Linearity is maintained within a wide concentration range from 2.00 to 25.00 mg/l, with relative error (-0.11 and 0.60) the LOD (0.360 and 0.382 mg/L) and LOQ and (1.200 and1.275 mg/L) respectively.No interference found between both determined and those of matrices. A good accuracy and precision of simultaneous determination of (SMX), and (TMP) were confirmed by statistical analysis.The percentage recovery of the individual drugs under the established conditions is ranged from 97.23% to 102.13 %. This method was compared with the British pharmacopoeiamethodusing F test. The procedures do not require any separation step.

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“…Several analytical methods, such as HPLC–UV [ 5 , 6 ], LC–MS [ 7 ] and capillary electrophoresis [ 8 ], have been developed to determine SMX and TMP in pharmaceutical formulations and biological fluids [ 9 , 10 , 11 , 12 ]. Most require relatively rigorous conditions; include serious sample-preparation steps which are expensive, time consuming and need hazardous solvents; and costly in terms of required instrumentation and well-trained technicians.…”

Section: Introductionmentioning

confidence: 99%

Application of Rank Annihilation Factor Analysis for Antibacterial Drugs Determination by Means of pH Gradual Change-UV Spectral Data

et al. 2020

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The main objective of this study was to develop a simple and efficient spectrophotometric technique combined with chemometrics for the simultaneous determination of sulfamethoxazole (SMX) and trimethoprim (TMP) in drug formulations. Specifically, we sought: (i) to evaluate the potential use of rank annihilation factor analysis (RAFA) to pH gradual change spectrophotometric data in order to provide sufficient accuracy and model robustness; and (ii) to determine SMX and TMP concentration in drug formulations without tedious pre-treatments such as derivatization or extraction techniques which are time-consuming and require hazardous solvents. In the proposed method, the spectra of the sample solutions at different pH values were recorded and the pH-spectra bilinear data matrix was generated. On these data, RAFA was then applied to estimate the concentrations of SMX and TMP in synthetic and real samples. Applying RAFA showed that the two drugs could be determined simultaneously with concentration ratios of SMX to TMP varying from 1:30 to 30:1 in the mixed samples (concentration range is 1–30 µg mL−1 for both components). The limits of detection were 0.25 and 0.38 µg mL−1 for SMX and TMP, respectively. The proposed method was successfully applied to the simultaneous determination of SMX and TMP in some synthetic, pharmaceutical formulation and biological fluid samples. In addition, the means of the estimated RSD (%) were 1.71 and 2.18 for SMX and TMP, respectively, in synthetic mixtures. The accuracy of the proposed method was confirmed by spiked recovery test on biological samples with satisfactory results (90.50–109.80%).

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Development and validation of an HPLC method for simultaneous determination of trimethoprim and sulfamethoxazole in human plasma

Cited by 26 publications

References 13 publications

A Reliable Method to Quantify Trimethoprim and Sulfamethoxazole in Plasma of Children by HPLC with Application in Pharmacokinetic Studies

A Reliable Method to Quantify Trimethoprim and Sulfamethoxazole in Plasma of Children by HPLC with Application in Pharmacokinetic Studies

Simultaneous Determination of Sulphamethoxazole and Trimethoprim in Binary Mixtures and in Tablet Using Derivative Spectrophotometry

Application of Rank Annihilation Factor Analysis for Antibacterial Drugs Determination by Means of pH Gradual Change-UV Spectral Data

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