Development and validation of an analytical method for quantification of total flavonoids in Alternanthera brasiliana by ultraviolet-visible absorption spectrophotometry

Abstract

Alternanthera brasiliana is popularly known in Brazil as "penicillin" or "benzetacil" and is used in traditional medicine for the treatment of infections and for the healing of wounds. It is also used as an ornamental plant due to the characteristic purple coloration of its leaves when cultivated in shade. The objective of this study was to develop and validate an analytical methodology by Ultraviolet-Visible absorption spectroscopy to quantify total flavonoids in crude ethanolic extract of A. brasiliana. The parameters analyzed in validation were those indicated in resolution 166/2017 of ANVISA, as selectivity/specificity, linearity, accuracy, precision, robustness, limits of detection and quantification, and also by ICH Q2(R1) for analytical validation. The method developed was simple, fast, low cost, linear, selective, precise, accuracy and robust. All parameters analyzed were within the limits recommended by the Brazilian legislation. Thus, this methodology can be useful for quality control of the extract and vegetal derivatives of A. brasiliana.

Influence of organo-mineral supplementation on the production of secondary metabolites in in vitro-germinated Bromelia balansae Mez.

“…The quantification of total phenols was conducted according to a standard protocol described by Hagerman and Butler, wherein iron chloride is used as a chromogen and spectrophotometric reading is conducted at 510 nm, while the total phenols are calculated as tannic acid equivalents (Mole et al, 1987). The quantification of flavonoids followed the method described elsewhere using rutin equivalents and spectrophotometric readings conducted at 361 nm (José et al, 2019).…”