Alternanthera brasiliana is popularly known as "penicillin" and used as an antiinflammatory and for wound healing. The objective of this work was the phytochemical study of the species by analytical techniques such as LC-MS/MS and GC-MS. Twenty seven compounds were identified, five by LC-MS and twenty two by GC-MS analysis. All compounds identified by LC-MS are flavonoids. However, several classes were found in GC-MS analysis, such as hydrocarbons, diterpenes, monoterpenes, vitamin and carotenoid derivatives, phytosterols and triterpenes. In the analysis, it is suggested the presence of twenty-seven substances, of which twenty-three are unpublished for the species, that reaffirms the importance of this study and the contribution to chemical knowledge.

Alternanthera brasiliana is popularly known in Brazil as "penicillin" or "benzetacil" and is used in traditional medicine for the treatment of infections and for the healing of wounds. It is also used as an ornamental plant due to the characteristic purple coloration of its leaves when cultivated in shade. The objective of this study was to develop and validate an analytical methodology by Ultraviolet-Visible absorption spectroscopy to quantify total flavonoids in crude ethanolic extract of A. brasiliana. The parameters analyzed in validation were those indicated in resolution 166/2017 of ANVISA, as selectivity/specificity, linearity, accuracy, precision, robustness, limits of detection and quantification, and also by ICH Q2(R1) for analytical validation. The method developed was simple, fast, low cost, linear, selective, precise, accuracy and robust. All parameters analyzed were within the limits recommended by the Brazilian legislation. Thus, this methodology can be useful for quality control of the extract and vegetal derivatives of A. brasiliana.

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Morus nigra L. is known in the region of Brazilian São Francisco Valley as "amora-miúra". It is widely used in traditional medicine, mainly in treatment of diabetes, hypercholesterolemia, cardiovascular problems, obesity and gout. This study proposed to develop and validate a fast, simple, efficient and low cost analytical method that could quantify total flavonoid content present in crude ethanolic extract of leaves of M. nigra (Mn EtOH). For this, ultraviolet-visible (UV-Vis) absorption spectrophotometry was used as the analytical tool and the complexation with aluminium chloride (AlCl 3 ). The quantification of flavonoids by this method was based on the complexation of AlCl 3 with the flavonoid nucleus as a selectivity tool made by two procedures, without complexation (NCP) and with complexation (WCP), in order to evaluate the effects of the complexing agent in quantification, proving the efficiency of this technique. In this way, it became necessary to validate the method used to ensure its efficiency. The validation of the method of quantification of total flavonoid content by UV-Vis demonstrated that the method was selective, linear, precise, accurate and robust.