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Alternanthera brasiliana is popularly known as "penicillin" and used as an antiinflammatory and for wound healing. The objective of this work was the phytochemical study of the species by analytical techniques such as LC-MS/MS and GC-MS. Twenty seven compounds were identified, five by LC-MS and twenty two by GC-MS analysis. All compounds identified by LC-MS are flavonoids. However, several classes were found in GC-MS analysis, such as hydrocarbons, diterpenes, monoterpenes, vitamin and carotenoid derivatives, phytosterols and triterpenes. In the analysis, it is suggested the presence of twenty-seven substances, of which twenty-three are unpublished for the species, that reaffirms the importance of this study and the contribution to chemical knowledge.

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