Development and validation of an HPLC method for the determination of spironolactone and its metabolites in paediatric plasma samples

Determination of Spironolactone and Canrenone in Human Plasma by High-performance Liquid Chromatography with Mass Spectrometry Detection

“…Numerous HPLC methods have been developed for SPR and its metabolites determination in plasma or serum, 2 and only a few used liquid chromatography coupled with mass spectrometry as analytical tool for this kind of determination. 3,4 However, an important number of papers in which LC-MS determination of SPR from urine (doping agents screening) and milk are described.…”

Analytical issues in HPLC/MS/MS simultaneous assay of furosemide, spironolactone and canrenone in human plasma samples

“…Spironolactone, along with its main metabolites, canrenone and 7-␣-thiomethyl spironolactone, is usually detected through MS or MS/MS under APCI conditions [9], although UV detection is achievable if an adequate concentration technique (such as solid phase extraction) is used during sample preparation [10].…”

Development and validation of a dried blood spot—LC–APCI–MS assay for estimation of canrenone in paediatric samples

“…Several HPLC methods have been reported for estimation of CAN in various biomatrices such as serum, plasma and urine [10][11][12][13][14][15][16][17][18][19][20][21][22][23][24][25][26], however there are no published methods which have employed whole blood in liquid form or in the form of DBS. Many of the methods have several limitations such as lack of selectivity [10,11], low sensitivity [13,17] or large volume of biomatrix required (1 ml plasma) [18,25] and as such are not suitable for estimation of CAN in very low volume paediatric blood samples.…”

“…Many of the methods have several limitations such as lack of selectivity [10,11], low sensitivity [13,17] or large volume of biomatrix required (1 ml plasma) [18,25] and as such are not suitable for estimation of CAN in very low volume paediatric blood samples. We have previously reported a sensitive HPLC method for estimation of spironolactone and its metabolites, including CAN, in paediatric plasma [24] employing 200 l plasma samples however, the same method cannot be extrapolated to DBS samples as the volume of biomatrix to be processed is approximately 20 times lower than that utilised for plasma.…”