Development and Validation of an RP-HPLC Method for Determination of Solasodine, A Steroidal Alkaloid

Abstract

Objective:The aim of the present study was to optimize an RP-HPLC method for determination of solasodine, a steroidal alkaloid with various pharmacologic properties, in herbal medicinal preparations. Methods: Various chromatographic parameters including the composition of mobile phase and its organic and pH modifiers, mode of elution and column temperature were investigated using analytical C 18 columns as stationary phase. Acetonitrile and methanol were examined in combination with deionized water and different aqueous buffers as mobile phases. Results: A mixture of methanol: KH 2 PO 4 buffer (pH 2.5) at the ratio of 75:25 %v/v in an isocratic elution mode produced more symmetric, sharper peaks with better retention times and capacity factor than that of acetonitrile: KH 2 PO 4 buffer. The optimum column temperature was found at 25°C. The standard calibration curve showed a very good linearity (R 2 =0.9966) in the range of 1-25 μg/ml with acceptable inter-day and intra-day precisions and accuracy.In addition, the LOD and the LOQ of the method were determined at 0.2 and 0.7 μg/mL, respectively. Conclusion: This developed and validated method is simple and quite rapid and can be used for routine determination of solasodine in samples such as traditional medicinal preparations.This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.

Genetic variability, association and path analysis for yield and fruit quality components in yellow- berried nightshade (Solanum surattense)

“…The powdered samples were extracted using Microwave-assisted extraction with parameters of methanol: 70%, microwave power: 160 W, and time: 100 s (Gu et al, 2019). The technique used by Khandani et al (2019) was used to extract and analyse the alkaloid solasodine in HPLC (LC-20A) of Shimadzu make. The solvent system was a 30% HPLC gradient of water (20 mM phosphate buffered pH 3.5) (A) and 70 % methanol (B) for 15 min followed by washing for 30 min; injection sample volume was 10µl.…”

Antiparasitic Activity of Single Solanum ferox Extract and Concoction with Zingiber zerumbet and Boesenbergia pandurata Extracts to Control Argulus sp. on Goldfish (Cyprinus carpio)

“…One of the potential antiparasitic plants is sour eggplant, which contains solasodine, a steroidal glycoalkaloid compound. Some studies have shown that solasodine has antifungal [19,20], antibacterial [21][22][23][24]47,48], anticancer [25,26,45,46], and antiparasitic potencies as well as several other important activities [27].…”