Development and Validation of an Automated Liquid Handling System for Sample Preparation Based on Multichannel Air Displacement Pipetting Technology

Wu, Yanqi; Chen, Hui; Wang, Wei; He, Nongyue; Liu, Bin

doi:10.1166/jnn.2016.12597

Cited by 6 publications

(3 citation statements)

References 44 publications

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“…The individual components of the system can also be used for biomedical applications. Wu et al [13] presented a high-throughput automated sample processing system based on the principle of air displacement with an 8-channel pipetting arm. The platform could process 96 samples with a capacity of 10 to 350 µL simultaneously while meeting the standards set by the ISO-8655 [14] and JJG 646-2006 [15].…”

Section: Introductionmentioning

confidence: 99%

Model-Based Feedback Control for an Automated Micro Liquid Dispensing System Based on Contacting Droplet Generation through Image Sensing

Qian,

Xu,

Tian

et al. 2023

Micromachines

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Over the past few decades, micro liquid dispensing technology has been widely used in biology, chemistry, material and environmental sciences due to its efficacy in processing multiple samples. For practical applications, precise and effective droplet generation is very important. Despite numerous droplet generation methods, the implementation of droplet-on-demand still faces challenges concerning system complexity, precision, cost, and robustness. In this work, a novel on-demand contacting droplet generation method incorporated with model-based feedback control with an image processing unit as a sensor was proposed. By studying droplet identification using image processing techniques, the model of droplet formation was simplified. Then model-based feedback control was implemented using volumes of dispensed samples as sensing signals by tuning related parameters adaptively to resist disturbances. The proposed method was integrated and applied to a homebuilt automated micro liquid dispensing system with droplets ranging from 20 nanoliter to 200 nanoliter. The experimental results demonstrated a high degree of accuracy and precision. Additionally, the proposed system’s practical utility was evaluated by analyzing mutations in genes associated with sensorineural hearing loss, verifying its effectiveness.

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Section: Introductionmentioning

confidence: 99%

Model-Based Feedback Control for an Automated Micro Liquid Dispensing System Based on Contacting Droplet Generation through Image Sensing

Qian,

Xu,

Tian

et al. 2023

Micromachines

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“…Many aspects of the experiments carried out in these laboratories, such as sample preparation, dilution, 1 transfer, 2 dispense, and wash, require some form of automated liquid handling products and instruments. Some of these liquid handling systems are highly automated [3][4][5] for achieving high efficiency, accuracy, and precision 6 and are required for applications such as the development of new drugs 7 for pharmaceutical companies, analysis of DNA sequences 8 to monitor gene activity, immunoassay, 9 lab-on-chip applications, 10 cell sorting, 11 and others. Accurate and precise liquid dispense is a mission critical for these experiments and is achieved in many different methods.…”

Section: Introductionmentioning

confidence: 99%

A Low-Cost, Accurate, and High-Precision Fluid Dispensing System for Microscale Application

2017

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We present here, the development of a low cost, accurate and precise fluid dispensing system. It can be used with peristaltic or any other pump to improve the flow characteristics. The dispensing system has a range of 1–100 μL with accuracy of ~ 99.5% and standard deviation at ~150 nL over the entire range. The system developed does not depend on the accuracy or precision of the driving pump; thereby any positive displacement pump can be used to get similar accuracy and precision, which gives an opportunity to reduce the cost of the system. The dispensing system does not require periodic calibration and can also be miniaturized for microfluidic application. Though primarily designed for aqueous liquid, it can be extended for different non-conductive liquids as well with modifications. The unit is further used for near real-time measurement of Lactate from microdialysate. The individual components can easily be made disposable or sterilized for use in biomedical application.

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“…with strong practicability and a number of loci can be varied simultaneously detected, [62][63][64][65][66][67][68][69][70][71][72] based on a new SNP detection approach established at our laboratory.…”

Section: Introductionmentioning

confidence: 99%

Design and Implementation of Experimental Consumable Management Device for the Automatic Nucleic Acid Detection System

Jun¹,

Zhu²,

Wang³

et al. 2016

j nanosci nanotechnol

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A management device for experimental consumable based on the STM32 is designed to improve the efficiency of nucleic acid detection and its application for the automatic nucleic acid detection system based on magnetic nanoparticles is checked. The STM32 controller is used as the core of the management control system, the workflow and classification management of the experimental consumables is realized by STM32 controlling. The positioning of the consumables is controlled with the help of photoelectric sensor, which makes the device a closed loop control system. When received instructions from the host computer through RS485 bus, the device can analyze protocols and take appropriate measures such as pushing, recycling and recording experimental consumables. A single channel of cabinet structure is used as the primary structure of the experimental consumable management to save the space of the storage and improve the efficiency of the consumable management. This device provides researchers with quick and simple centralized control of diverse experimental consumables, which has the advantages of intelligence, remote, without human intervention, etc.

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Development and Validation of an Automated Liquid Handling System for Sample Preparation Based on Multichannel Air Displacement Pipetting Technology

Cited by 6 publications

References 44 publications

Model-Based Feedback Control for an Automated Micro Liquid Dispensing System Based on Contacting Droplet Generation through Image Sensing

Model-Based Feedback Control for an Automated Micro Liquid Dispensing System Based on Contacting Droplet Generation through Image Sensing

A Low-Cost, Accurate, and High-Precision Fluid Dispensing System for Microscale Application

Design and Implementation of Experimental Consumable Management Device for the Automatic Nucleic Acid Detection System

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