Development and Validation of an HPLC Method for Mefloquine Hydrochloride Determination in Tablet Dosage Form

Abstract

A simple HPLC method for determination of mefloquine hydrochloride in tablets was developed and validated. The separation was carried out on an Xterra RP18 (250 × 4.6 mm id, 5 µm particle size) analytical column. The mobile phase was 0.05 M monobasic potassium phosphate buffer (pH 3.5)–methanol (40 + 60, v/v). The flow rate and wavelength were set to 1 mL/min and 283 nm, respectively. The method was specifc for mefloquine hydrochloride in the presence of hydrolytic, oxidative, and photolytic degradation products. It was also linear, precise, accurate, and robust, being suitable for routine QC analyses and stability studies. The developed HPLC method was compared to a previously described spectrophotometric method.

Validation of a straightforward high performance liquid chromatographic method for morphine quantitation

“…A routinely used chemical, 2,2,2 triphenylacetophenone could have been incorporated for this purpose but it was subsequently dismissed because the late eluting opiates tended to interfere with it. Similar to some studies that employed no IS for their quantitative work (Lee et al 2005 ; Nogueira et al 2011 ), a quantitative method for morphine was developed in the external standard mode. This is justifiable because the method does not involve extraction whereas the signal response (the peak area) is sufficiently high to overcome insignificant errors associated with the overall method.…”

Validation of a UV Spectrophotometric Method for Determination of Proteolytic Activity of Enzymatic Detergents

“…This method was validated through the evaluation of the same analytical parameters used for drugs and pharmaceutical preparations, i.e. specificity, linearity, detection and quantification limits, [12][13][14].…”