Development and validation of BLRI Mastitis Test Kit at Bangladesh Livestock Research Institute Regional Station, Sirajganj

Kabir, Md. Humayun; Ershaduzzaman, Md.; Nazir, K. H. M. Nazmul Hussain; Islam, Mohammad Sirajul; Khatun, Razia; Sarker, Samun; Yousuf, Md. Abu; Ali, Yousuf; Sarkar, Nathu Ram; Giasuddin, M

doi:10.5455/javar.2019.f363

Cited by 4 publications

(3 citation statements)

References 18 publications

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“…Importantly, the costing and ease of access of the prototype reagents was lower as compared to commercially available mastitis test. Previous studies have demonstrated that screening of milk to detect subclinical mastitis is one of the most accurate methods (Kabir et al, 2019). Therefore, the newly developed milk screening reagent can be an independent, cheap and farmer friendly and alternative subclinical mastitis screening testing reagent readily available for use by rural livestock owners.…”

Section: Discussionmentioning

confidence: 99%

Development of a prototype milk screening reagent for the detection of bovine subclinical mastitis using common detergents and Hibiscus sabdariffa petals

2022

JoSVAS

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Mastitis is an inflammatory condition of the mammary gland of female animals which can either be clinical or subclinical. Subclinical mastitis is that which cannot be diagnosed by physical assessment of the udder, however, it continues to reduce the quality and quantity of the milk leading to economic losses. Diagnosis and treatment of subclinical mastitis is therefore of paramount importance to the farmer, however, the commercially available California Mastitis Test (CMT) reagent commonly used to detect subclinical mastitis is expensive and not readily accessible especially in the rural agrarian communities. This work was conducted to develop a test reagent from locally and readily available materials that can be used by rural farmers in the detection of subclinical mastitis. Different concentrations (1.5%, 3% and 6%) of different household detergents dissolved in water extract of Hibiscus sabdariffa (zobo) have been explored for their suitability to detect somatic cells in cow milk. The reagents were mixed with mastitic and nonmstatitic milk while the test conducted with CMT served as control to which the results from formulated reagents were compared for sensitivity. It was found that a 3% detergent concentration in zobo can be used to obtain gel formation and a colour change from red to dark green corresponding to somatic cell counts and pH respectively, much like the CMT reagent. The test conducted revealed 53.57%, 46.42%, 42.85% and 42.85% of cows’ fore-quarter milk samples were positive to CMT, detergents A, B and C respectively. The combination of household detergent and water extract of Zobo can therefore be an alternative to CMT reagent in the detection of subclinical mastitis, especially in the rural areas. This indicates the potential of using these test reagents for subclinical mastitis screening. Further studies are suggested to standardize and patent this reagent as an alternative for subclinical mastitis diagnostic test.

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Section: Discussionmentioning

confidence: 99%

Development of a prototype milk screening reagent for the detection of bovine subclinical mastitis using common detergents and Hibiscus sabdariffa petals

2022

JoSVAS

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“…Similarly, CMT often has poor sensitivity on detecting subclinical mastitis and the yielded result could be difficult to interpret (Viguier et al, 2009). Early detection of subclinical mastitis is crucial to prevent the source of infection for herd mates, prevent transmission of zoonotic pathogens to human and minimize economic losses (Kabir et al, 2019).…”

Section: Investigation Of Serum and Milk Acute Phase Proteins Level I...mentioning

confidence: 99%

Investigation of Serum and Milk Acute Phase Proteins Level in Goats with Experimentally Infected Staphylococcus aureus Subclinical Mastitis

Ariffin¹,

Hian²,

Sukiman³

et al. 2021

JAHP

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Mastitis is an inflammatory condition of the mammary gland which is usually caused by a bacterial infection. This study aims to determine acute phase protein (APP) and its association with somatic cell count (SCC) during experimentally induced subclinical mastitis in goats. Thirty lactating goats were divided into two groups (n=15 per group) challenged either by intramammary infusion of 1 x 10 3 cfu/mL Staphylococcus aureus (S. aureus) or phosphate-buffered saline (control). The haptoglobin (Hp), serum amyloid A (SAA) and α1-acid glycoprotein (AGP) levels in serum and milk were measured at pre-and post-infection (6, 24, 48 and 72 h) using the ELISA method. The SCC was determined by the direct microscopic method. Results revealed a time-dependent change of Hp, SAA and AGP in the infected group compared to the control. The APP levels in the milk were significantly higher (p<0.05) than those in the serum. A significant good correlation between milk Hp concentration and SCC was observed (r s = 0.73, p<0.05). However, there were no associations between milk SAA and AGP concentrations with SCC respectively. In conclusion, altered expression of the serum and milk APPs was seen during S. aureus-induced subclinical mastitis infection in goats, which unlock the potentials of APP evaluation as alternative tools for early diagnosis of subclinical mastitis in goats. Also, there was a significant relationship between milk Hp and SCC, thus reflecting the intensity of the inflammatory response to mammary tissue.

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“…Immediately following the elevation of SCC in milk, the activity of proteolytic enzymes dramatically increases [10]. Automatic electronic counters and CMT are the most accurate and widely used methods for determining the amount of SCC [11]. During inflammation, mastitis releases several proteases and recruits polymorphonuclear neutrophils (PMNs) to migrate across the blood/milk barrier [12].…”

Section: Introductionmentioning

confidence: 99%

The influence of subclinical mastitis on the protein composition and protease activities of raw milk from lactating Thai-crossbred dairy cows

et al. 2023

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Background and Aim: Mastitis in dairy cattle is associated with a high rate of morbidity and death, which has major implications for milk production and quality. This study aimed to investigate the protein component and the activity of matrix metalloproteinase-2 (MMP-2) and -9 (MMP-9) in raw milk samples with different testing scores determined using the California mastitis test (CMT). Materials and Methods: Thirty cows were employed in the study, and milk from each quarter was tested for subclinical mastitis (SCM). According to the results of CMT, raw milk samples were classified into five categories: Healthy (score 0), trace (score T), weakly positive (score 1), distinctly positive (score 2), and strongly positive (score 3) for somatic cell count (SCC). The total milk protein was analyzed using the Bio-Rad protein assay, and the milk protein composition was determined using the sodium dodecyl sulfate-polyacrylamide gel electrophoresis technique. In addition, gelatin zymography was used to evaluate changes in proteolytic abilities. Results: Milk samples with CMT scores of 1 and 3 had the highest total milk protein levels (32.25 ± 12.60 g/L and 32.50 ± 7.67 g/L, respectively), while the samples from healthy cows (CMT score 0) were only 6.75 ± 1.64 g/L. Globulin and lactoferrin were significantly increased in samples with a CMT score of 3 compared with those with other CMT scores. The bovine serum albumin level in samples with a CMT score of 2 was significantly (p < 0.05) higher than those with other CMT scores. No significant differences in casein abundance were found among samples with different CMT scores. Results from analysis of proteolytic activities demonstrated that the level of MMP-9 in samples with a CMT score of 3 was significantly (p < 0.05) higher than those with other CMT scores. Conclusion: The protein content and gelatinolytic activity of milk were drastically altered by the number of SCC, mainly due to SCM. Keywords: milk protein, protease activity, subclinical mastitis, Thai-crossbred dairy cows.

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Development and validation of BLRI Mastitis Test Kit at Bangladesh Livestock Research Institute Regional Station, Sirajganj

Cited by 4 publications

References 18 publications

Development of a prototype milk screening reagent for the detection of bovine subclinical mastitis using common detergents and Hibiscus sabdariffa petals

Development of a prototype milk screening reagent for the detection of bovine subclinical mastitis using common detergents and Hibiscus sabdariffa petals

Investigation of Serum and Milk Acute Phase Proteins Level in Goats with Experimentally Infected Staphylococcus aureus Subclinical Mastitis

The influence of subclinical mastitis on the protein composition and protease activities of raw milk from lactating Thai-crossbred dairy cows

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