Development and Validation of High Performance Liquid Chromatography Method for Simultaneous Estimation of Flavonoid Glycosides in Withania somnifera Aerial Parts

Abstract

Withania somnifera (L.) Dunal (Solanaceae) commonly known as ashwagandha, is an important plant in Ayurveda and is believed to increase longevity and vitality. The root is considered to be the medicinally important part of the plant as per classical texts and accordingly is the subject of most Pharmacopeial monographs. The aerial parts, being less expensive, are sometimes mixed with roots to prepare “standardized” extracts of W. somnifera, and in cases with false declaration of plant part used as roots on the certificate of analysis. The present study described a new, simple, accurate, and precise HPLC method for the simultaneous determination of flavonoid glycosides as unique constituents of the aerial parts, being absent in roots of the plant. The RSD for intra- and interday analyses was less than 2.5% and the recovery was 90–108%. The method was used to analyze samples of roots and aerial parts of the plant collected from India and Egypt. The samples of commercially available extracts of W. somnifera were also analyzed and many samples were found to contain flavonoid glycosides indicating a possible undeclared use of aerial parts in the extracts derived from roots in commercial practice.

Red light controlled callus morphogenetic patterns and secondary metabolites production in Withania somnifera L.

“…HPLC analysis for withanolides was performed with some modification to the method of Mundkinajeddu et al [25]. The Jasco HPLC system (LC-Net II), consisting of a quaternary pump (PU-2089), an autosampler (AS-2059 Plus) and a PDA detector (MDA-2018) was used for putative detection of withanolides.…”

Withania somnifera phytochemicals possess SARS-CoV-2 RdRp and human TMPRSS2 protein binding potential

“…Recently, in silico antiviral activity of galactitol against Ebola virus has been reported (Nagrajan et al 2019). Quercetin 3-rutinoside-7-glucoside is a flavonoid glycoside identified in W. somnifera aerial part (Mundkinajeddu et al 2014). Antiviral potential of flavonoid glycoside has been reported against human coronaviruses (Schwarz et al 2014).…”

Quality control and standardization of Quercetin in herbal medicines by spectroscopic and chromatographic techniques

“…Deepak Mundkinajeddu et al, have developed a method for estimation of the flavonoid glycoside in Withenia somnifera by HPLC using Phenomenex Luna C18 column (5µ, 250 × 4.6 mm) and the mobile system consist of potassium dihydrogen orthophosphate (0.136 g) dissolved in 900 ml of HPLC grade water to that 0.5 ml of orthophosphoric acid added volume made up to 1000 ml. the method for the estimation of 3 flavonoid glycosides that are quercetin-3-orobinoside-7-Oglucoside (1), quercetin-3-O-rutinoside-7-O-glucoside (2), kaempferol 3-O-robinobioside-7-O-glucoside [16].…”