Development and Validation of LC–MS Method for the Determination of Hydroxyzine Hydrochloride in Human Plasma and Subsequent Application in a Bioequivalence Study

Zhou, Neng; Liang, Yi‐Zeng; Chen, Ben-Mei; Wang, Ping; Cheng, Xian; Fengping, Liu

doi:10.1365/s10337-007-0372-x

Cited by 14 publications

(3 citation statements)

References 15 publications

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“…Considering the similarity of the conditions of determination the specific transition for the analysis of 3-hydroxydesloratadine is 326.9 (m/z) -> 274.98 (m/z) with collision energy of 27 eV. Hydroxyzine may be a source of cetirizine, as a metabolite [22]. The specific transition used was 375 (m/z) -> 201 (m/z).…”

Section: Selectivitymentioning

confidence: 99%

LC-MS/MS Bioanalytical Method for Simultaneous Quantification of Three H1-Antihistaminic Drugs in Human Plasma

2019

Rev.Chim.

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The aim of the study was to validate a bioanalytical method, for quantifying desloratadine, loratadine and cetirizine in biological samples, useful in toxicological studies. The analytical performance was investigated using the following parameters: selectivity, limit of detection and quantification, linearity, precision, accuracy, matrix effect and analytical stability in the biological matrix. The method optimization was performed for the LC-MS/MS Ultimate 3000 instrument provided by Thermo Fisher Scientific. Keywords: H1-antihistaminic drugs, human plasma, LC-MS/MS

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Section: Selectivitymentioning

confidence: 99%

LC-MS/MS Bioanalytical Method for Simultaneous Quantification of Three H1-Antihistaminic Drugs in Human Plasma

2019

Rev.Chim.

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“…kütle spektrometresi (LC-MS) [13]; potansiyometri [14][15]; gravimetri [16], titrimetri [17][18][19] ve ters faz sıvı kromatografi (RP-HPLC) [20][21] gibi pek çok farklı yöntemle analizi gerçekleştirilmiştir. Ters faz sıvı kromatografi haricinde bildirilen yöntemlerin çoğu, analizden önce numune ön işlemi ve ilaç ekstraksiyonu gerektirmektedir.…”

Section: Introductionunclassified

Hidroksizinin Tablet Formülasyonunda Analizi için Yeni Bir RP-HPLC Tekniği

Nane

2023

Süleyman Demirel Üniversitesi Sağlık Bilimleri Dergisi

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Alerji ilaçları, alerjik reaksiyonları hafifletmek ve semptomları kontrol altına almak için kullanılan farmasötik ürünlerdir. Günümüzde alerji ilaçlarının önemi, artan alerjik hastalık prevalansı ve semptomların etkili bir şekilde yönetilmesinin gerekliliği nedeniyle giderek artmaktadır. Bu araştırmada alerjik bir ilaç olan hidroksizinin tayin edilmesinde yeni bir metot önerilmiştir. Bu araştırmanın amacı, tablet formundaki piperazin türevi ilaç etken maddesi hidroksizini analiz etmek için bir RP-HPLC metodu geliştirmek ve doğrulamaktır. Optimum sıvı kromatografik ayırma koşullarını belirlemek için mobil faz pH'ı ile alıkonma süresi arasındaki korelasyona dayanan sistematik bir yaklaşım kullanılmıştır. Hidroksizinin tayini, Xbridge C18 kolonunda (3 um, 150 x 4,6 mm, I.D.), pH 6'ya ayarlanmış %45 (v/v) asetonitril içeren asetonitril-su ikili karışımında sağlanmıştır. Çalışma 0,8 mL/dakika akış hızında, 37 °C kolon sıcaklığı ve 230 nm dalga boyunda gerçekleştirilmiştir.

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“…Various analytical methods including HPLC , LC‐MS , voltammetry , GC , thin layer chromatography (TLC) , micellar‐liquid chromatography , CZE , plasticized polyvinylchloride membrane‐based potentiometry , and conductometric titration have been reported in the literature for the determination of HZ in its pharmaceutical preparations and human serum. Also, a number of analytical methods have been used to quantify CTZ in pharmaceutical formulations and biological fluids including high performance thin layer chromatography (HPTLC) , GC , HPLC with UV detection , HPLC with fluorescence detection , HPLC with on‐line liquid scintillation counting , and CE .…”

Section: Introductionmentioning

confidence: 99%

Two‐step liquid phase microextraction combined with capillary electrophoresis: A new approach to simultaneous determination of basic and zwitterionic compounds

Nojavan

Moharami²,

Fakhari

2012

J of Separation Science

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In this work, two-step hollow fiber-based liquid-phase microextraction procedure was evaluated for extraction of the zwitterionic cetirizine (CTZ) and basic hydroxyzine (HZ) in human plasma. In the first step of extraction, the pH of sample was adjusted at 5.0 in order to promote liquid-phase microextraction of the zwitterionic CTZ. In the second step, the pH of sample was increased up to 11.0 for extraction of basic HZ. In this procedure, the extraction times for the first and the second steps were 30 and 20 min, respectively. Owing to the high ratio between the volumes of donor phase and acceptor phase, CTZ and HZ were enriched by factors of 280 and 355, respectively. The linearity of the analytical method was investigated for both compounds in the range of 10-500 ng mL(-1) (R(2) > 0.999). Limit of quantification (S/N = 10) for CTZ and HZ was 10 ng mL(-1) , while the limit of detection was 3 ng mL(-1) for both compounds at a signal to noise ratio of 3:1. Intraday and interday relative standard deviations (RSDs, n = 6) were in the range of 6.5-16.2%. This procedure enabled CTZ and HZ to be analyzed simultaneously by capillary electrophoresis.

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Development and Validation of LC–MS Method for the Determination of Hydroxyzine Hydrochloride in Human Plasma and Subsequent Application in a Bioequivalence Study

Cited by 14 publications

References 15 publications

LC-MS/MS Bioanalytical Method for Simultaneous Quantification of Three H1-Antihistaminic Drugs in Human Plasma

LC-MS/MS Bioanalytical Method for Simultaneous Quantification of Three H1-Antihistaminic Drugs in Human Plasma

Hidroksizinin Tablet Formülasyonunda Analizi için Yeni Bir RP-HPLC Tekniği

Two‐step liquid phase microextraction combined with capillary electrophoresis: A new approach to simultaneous determination of basic and zwitterionic compounds

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