Development and Validation of Rapid LC-MS with Electrospray Ionization for the Quantification of Pramipexole in Human Plasma

Abstract

A rapid, accurate and precise LC-MS method is described for the quantitative determination of pramipexole in human plasma matrix using ropinirole as internal standard. Pramipexole and ropinirole were extracted from plasma by liquid-liquid extraction technique. The method was validated over the concentration range of 100-2514 pg/mL. The method was found to have acceptable accuracy, precision, linearity and selectivity. The mean extraction recovery from spiked plasma samples was in the range of 79.415-87.00 %. The intra-day accuracy of the assay ranged from 98.924 to 112.236 % and intra-day precision ranged from 3.489 to 6.756 %. Inter-day accuracy and precision results for quality control samples ranged between 100.340 and 107.443% of nominal and precision is observed to be 3.970-5.714 %. The pramipexole was found to be stable after several stability studies. The proposed method yielded a quick, simple and reliable protocol for estimating pramipexole concentrations in human plasma.

Novel contribution to the simultaneous monitoring of pramipexole dihydrochloride monohydrate and levodopa as co-administered drugs in human plasma utilizing UPLC–MS/MS

“…In spite that several HPLC–MS/MS methods were performed for the determination of PDM 15–22 and LVD 39–42 singly in human plasma, yet, our proposed method is the first performed attempt for their simultaneous determination as was indicated from a thorough literature survey. The extraction technique and the chromatographic conditions of these reported methods are summarized in Table 4.…”

“…It is beneficial in reducing ''off'' periods with LVD and in ameliorating fluctuations in mobility in the later stages of the disease. 2 Several analytical techniques were established to quantify PDM as a single component, including amperometry, 3 voltammetry, 4 spectrophotometry, [5][6][7] high performance thin layer chromatography (HPTLC), 8 gas chromatography mass spectrometry (GC-MS), 9 high performance liquid chromatography (HPLC), [10][11][12][13][14] liquid chromatography tandem mass spectrometry (LC-MS/MS), [15][16][17][18][19][20][21][22] and capillary electrophoresis (CE). 23,24 LVD chemically is 3-hydroxyl-L-tyrosine 1 (Figure 1).…”