Development and validation of reversed phase liquid chromatographic method utilizing ultraviolet detection for quantification of irinotecan (CPT-11) and its active metabolite, SN-38, in rat plasma and bile samples: Application to pharmacokinetic studies

Bansal, Tripta; Awasthi, Anshumali; Jaggi, Manu; Khar, Roop K.; Talegaonkar, Sushama

doi:10.1016/j.talanta.2008.04.058

Cited by 46 publications

(32 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To determine the amount of SN-38 in the intestinal fluid, the methodology proposed by Bansal et al (2008) was followed with some modifications. Primary stock solutions for SN-38 (Sigma Aldrich) were prepared separately in DMSO at a concentration of 1 mg ml 21 .…”

Section: Methodsmentioning

confidence: 99%

Evaluation of mucositis induced by irinotecan after microbial colonization in germ-free mice

et al. 2015

View full text Add to dashboard Cite

Mucositis is one of the most debilitating side effects of chemotherapy and some previous studies suggest a role for indigenous microbiota in the course of this pathology. Therefore, the aim of our study was to evaluate the differences in phenotype between germ-free (GF) and conventional (CV) mice, and the role of b-glucuronidase-producing bacteria in the development of irinotecan treatment in a murine model. After mucositis induction, CV mice showed a significant increase in all inflammatory parameters when compared to GF mice. CV animals also showed more lesions of the intestinal epithelium, coherent with their higher intestinal permeability. The conventionalization of GF animals reversed their phenotype to that found in CV mice. In addition, gnotobiotic mice monoassociated with an Escherichia coli strain producing b-glucuronidase showed an increased permeability when compared to gnotobiotic mice monoassociated with an E. coli strain deleted for the gene encoding b-glucuronidase, but these did not show any differences in the influx of neutrophils, eosinophils or histological characteristics. Our data confirmed that components of the gut microbiota are involved in the signs of mucositis. Nevertheless, other mechanisms than this enzyme are involved in the irinotecan treatment, since the monoassociation was not able to restore the entire phenotype observed in the CV animals with irinotecan treatment in our murine model.

show abstract

Section: Methodsmentioning

confidence: 99%

Evaluation of mucositis induced by irinotecan after microbial colonization in germ-free mice

et al. 2015

View full text Add to dashboard Cite

show abstract

“…Theo btained LOD value for the anticancer drug Flu (1.55 10 À8 M) usingS WCASV at aP GE is substantially lower as compared with data reported previously at polymer film modified carbonp aste electrodes (1.81 10 À7 M) [41],ahangingm ercury drop electrode (1.90 10 À7 Ma nd 8.7 10 À8 M) [39],s pectrophotometric method (1.04 10 À6 Ma nd 4.77 10 À7 M) [32] and af low injection UV spectroscopy (4.34 10 À7 M) [33]. An improved detection limit was also achieved for the determination of the anticancer drug Irino (1.68 10 À9 M) at aP GE using the proposed procedure as compared with that obtained by HPLC( 2.41 10 À8 M) [20] and at polymethyleneb lue multiwalledc arbonn anotubesm odified glassy carbon electrode (2.14 10 À7 M) [43].I no rder to evaluate the accuracy and precision of the proposed method analysiso fF lu and Irino drugs at three levels of low,m oderate and high concentrations of the calibration curve was carried out in seven independent series at the same day (intra-day) and performed at the samel evel on seven different days (inter-day). Ther esults are summarized in Table 3.…”

Section: Validation Of the Swcasv Proceduref Or Trace Concentrations mentioning

confidence: 85%

Square Wave Cathodic Adsorptive Stripping Voltammetric Determination of the Anticancer Drugs Flutamide and Irinotecan in Biological Fluids Using Renewable Pencil Graphite Electrodes

2015

View full text Add to dashboard Cite

1IntroductionThea ntitumoral activity of flutamide {2-methyl-N-[4-nitro-3-(trifluoro methyl) phenyl] propanamide}( Flu) and irinotecan {7-ethyl-10-[4-(1-piperidino)1-piperidino] carbonyloxy-camptothecin} (Irino)( Scheme 1) against certain types of cancers was previously reported.Flutamide seems to have antiandrogenics pecificity only in androgend ependentg enitalia organs,a nd its therapeuticu se is in prostatic cancer [1][2][3][4].A sf lutamide has as tructure similar to testosterone,i tw orksb ya ttaching itself to the receptors on the surface of the cancer cells to block and prevent the attachment of the male hormone [5].I rinotecan is one of the key drugs of anticancer chemotherapy,e specially for the treatment of colorectal and lung tumors as well as other types of cancers [6,7].T he large scale therapeutic use of Flu and Irino generated the need for the development of fast, simple accuratem ethodologies for detection and quality control for both drugs in biological fluids. In this context methods reporting the determinationo fFlu and Irino include chromatography ,s pectrophotometry [31-35],s pectrofluorimetry [36] and few electrochemical methods [37][38][39][40][41][42][43].P olarographicr eduction of Flu was performed at ad ropping mercurye lectrode usingd irect currentp olarographya nd differential pulse polarography [37,38,40].L inear sweep and square waveforms were optimized for the determination of Flu based on the reduction of its nitro aromatic moiety at the hanging mercury drop electrode [39].D etermination of Flu ins urfactant containingm edia at polymer filmm odified carbon paste electrodes usingd ifferential pulsev oltammetryw as reported [41].E lectroanalytical determination of Irino was achieved using fast Fourier transform continuous cyclic voltammetry at gold microelectrodes in aflow system [42].V oltammetric determination of Irino at polymethylene blue multiwalled carbon nanotubes modified glassy carbone lectrode was reported as well [43].T ot he best of our knowledge,t he controlled adsorptive accumulationo fF lu or Irino on the surface of pencil graphite electrodes (PGE) for square wave cathodic adsorptive stripping determinationw as not reported yet. In view of the biological importance of Flu and Irino, this work aims on the development of as imple and sensiAbstract:Asensitive electrochemical method based on square wave cathodic adsorptive strippingv oltammetry (SWCASV) using pencil graphite electrodes( PGE)w as developed for the individual and simultaneous determination of the anticancer drugs flutamide( Flu)a nd irinotecan (Irino) in biological fluids.C alibration curves showed an excellent linear response with limitso fd etection of 1.68 10 À9 and 1.55 10 À8 MI rino and Flu,r espectively. Thes tatistical evaluation of within-day repeatability (n = 5) and day to day precision (n = 5) showed satisfactory accuracy and precision. SWCASV using aPGE for individual and simultaneous determination of both drugs in bulk form, human urine and serums amples was demonstrated.

show abstract

“…Method was found to be selective, linear (R 2 ≈0.999), accurate (recovery ± 15 %) and precise (<5 % C.V.) in the selected concentration ranges for both the analytes. The quantification limit for irinotecan was 40 ng ml -1 and for SN-38 was 25 ng ml -1 [6].…”

Section: Analytical Assay Proceduresmentioning

confidence: 89%

“…The concentrations of irinotecan and SN-38 in plasma were determined using high performance liquid chromatographic (HPLC) method using ultraviolet detection (λ max = 254 nm, 380 nm) as described previously [6]. The sample pre-treatment from plasma involved a single protein precipitation step with cold acetonitrile.…”

Section: Analytical Assay Proceduresmentioning

confidence: 99%

P-glycoprotein-dependent pharmacokinetics of irinotecan and its active metabolite, SN-38 in rats: Effect of verapamil

Garg¹,

Jagg²,

Khar³

et al. 2015

ADMET DMPK

Self Cite

View full text Add to dashboard Cite

We have recently demonstrated that the oral bioavailability of irinotecan (80 mg/kg) can be increased at least 7-fold by co-administration of the P-gp blocker verapamil (25 mg/kg, Oral). As a result, co-treatment with P-gp inhibitor could be a useful strategy for bioavailability enhancement. However, in view of narrow therapeutic index, the co-administration of irinotecan and verapamil may result in unanticipated toxicities. Therefore, dose optimisation studies of irinotecan were performed when it is given in conjunction with a P-gp inhibitor. For dose optimization study, the bioavailability and pharmacokinetic parameters were studied in rats after oral administration of irinotecan at three doses (i.e. 20, 40 and 80 mg/kg) alone and in combination with verapamil (25 mg/kg, oral 7.84 ± 1.20, 19.94 ± 2.39 and 61.71 ± 15.0 h µg/ml, respectively. In

show abstract

Development and validation of reversed phase liquid chromatographic method utilizing ultraviolet detection for quantification of irinotecan (CPT-11) and its active metabolite, SN-38, in rat plasma and bile samples: Application to pharmacokinetic studies

Cited by 46 publications

References 18 publications

Evaluation of mucositis induced by irinotecan after microbial colonization in germ-free mice

Evaluation of mucositis induced by irinotecan after microbial colonization in germ-free mice

Square Wave Cathodic Adsorptive Stripping Voltammetric Determination of the Anticancer Drugs Flutamide and Irinotecan in Biological Fluids Using Renewable Pencil Graphite Electrodes

P-glycoprotein-dependent pharmacokinetics of irinotecan and its active metabolite, SN-38 in rats: Effect of verapamil

Contact Info

Product

Resources

About