Development and Validation of RP-HPLC Method for Quantitative Estimation of Vinpocetine in Pure and Pharmaceutical Dosage Forms

Abstract

A simple, precise, specific, and accurate reversed phase high performance liquid chromatographic (RP-HPLC) method was developed and validated for determination of vinpocetine in pure and pharmaceutical dosage forms. The different analytical performance parameters such as linearity, accuracy, specificity, precision, and sensitivity (limit of detection and limit of quantitation) were determined according to International Conference on Harmonization ICH Q2 (R1) guidelines. RP-HPLC was conducted on Zorbax C18 (150 mm length × 4.6 mm ID, 5 μm) column. The mobile phase was consisting of buffer (containing 1.54% w/v ammonium acetate solution) and acetonitrile in the ratio (40 : 60, v/v), and the flow rate was maintained at 1.0 mLmin−1. Vinpocetine was monitored using Agilent 1200 series equipped with photo diode array detector (λ = 280 nm). Linearity was observed in concentration range of 160–240 μgmL−1, and correlation coefficient was found excellent (R2 = 0.999). All the system suitability parameters were found within the range. The proposed method is rapid, cost-effective and can be used as a quality-control tool for routine quantitative analysis of vinpocetine in pure and pharmaceutical dosage forms.

Preparation, characterization and in-vitro evaluation of theophylline loaded microemulsion formulations

“…where, σ = the standard deviation of the response s = slope of the calibration curve [24]. LOD and LOQ for theophylline were found to be 0.260 µg/mL and 0.786 µg/mL, respectively.…”

Phytochemical and Biological Evaluation of a Newly Designed Nutraceutical Self-Nanoemulsifying Self-Nanosuspension for Protection and Treatment of Cisplatin Induced Testicular Toxicity in Male Rats

“…The developed methods were validated in terms of linearity, accuracy (%recovery), precision, and sensitivity (LOD, LOQ) according to ICH guidelines, [ 30 , 31 , 32 ].…”

Identification and quantification of vinpocetine and picamilon in dietary supplements sold in the United States

“…More than 300 brands of supplements labelled as containing vinpocetine are available for sale in the USA. Vinpocetine is not found in nature but is closely related to vincamine, a natural and major component of leaves of the lesser periwinkle plant ( Vinca minor L) . Vinpocetine is a dehydrated derivative of vincamine in which the methyl ester is replaced by an ethyl ester (Figure ) and several approaches to synthesizing vinpocetine have been described …”

“…Vinpocetine is not found in nature but is closely related to vincamine, a natural and major component of leaves of the lesser periwinkle plant (Vinca minor L). [6][7][8][9] Vinpocetine is a dehydrated derivative of vincamine in which the methyl ester is replaced by an ethyl ester ( Figure 1) and several approaches to synthesizing vinpocetine have been described. [10][11][12][13] Picamilon (also known as nicotinoyl-GABA, pycamilon, and pikamilon) was developed by Russian investigators in an effort to increase central nervous system (CNS) levels of γ-aminobutyric acid (GABA).…”

“…[23][24][25] To our knowledge, the accuracy of the information available on the label regarding the quantities of these two drugs in dietary supplements has not been previously studied. Spectrophotometric and high performance liquid chromatographic (HPLC) methods have previously been used to determine the quantity of vinpocetine in laboratory-prepared mixtures and pharmaceutical preparations, [9,26] HPLC methods have been applied to detecting vinpocetine in biological matrices including human and rat plasma [27,28] and gas chromatographic-mass spectroscopy (GC-MS) methods [29] have been used for biological matrices such as human plasma. In respect to picamilon, previous researchers used HPLC to determine the quantity of picamilon in biological matrices such as human plasma samples.…”