Development and Validation of RP-HPLC Method for Determination of Doxorubicin Hydrochloride from Vacuum Foam Dried Formulation

A, Hajare Ashok; A, Powar Trupti; Neela, M. Bhatia; N, More Harinath

doi:10.5958/0974-360x.2016.00259.6

Cited by 10 publications

(2 citation statements)

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“…Calibration curves of melphalan were obtained by serial dilution of standard stock solution for five different concentrations (10,20,30,40 and 50 μg mL −1 ) by considering drug purity as 99.4% respectively, as indicated by the manufacturer. The solutions were injected thrice into HPLC column, keeping injection volume constant (20μL).…”

Section: Linearitymentioning

confidence: 99%

“…9 Melphalan was also detected from biological samples using LC-MS or HPLC or both the chromatographic methods. 10 When developing a nanosuspension it is essential to use an analytical procedure that allows optimal determination of the drug entrapment efficiency thereby enabling to assure that all the drug has been adequately extracted from the drug delivery system. 11 Taking all in consideration, the objective of present work is to develop and validate a new analytical method for quantification of melphalan from lyophilized nanosuspension.…”

Section: Introductionmentioning

confidence: 99%

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Development and Validation of an HPLC- UV Method for the Determination of Melphalan from Lyophilized Nanosuspension

Ashok¹,

Ananda²

2019

IJPER

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Aim and Objective: The objective of this work was to develop and validate a high performance liquid chromatography (HPLC) method for the quantitative analysis of melphalan, an anticancer drug from lyophilized nanosuspension. Material and Method: Chromatographic separation was achieved by using a reverse-phase C 18 column (150 mm ×4.6 mm, pore size 5 μm, Phenomenex). The mobile phase was optimized as acetic acid, water and methanol (1: 49.5: 49.5) with pH 4 at a flow rate of 1 mL/min. The melphalan was detected and quantitated using a UV detector at a wavelength of 254 nm. Result: The method was shown to be specific and linear in the range of 10-50 μg.mL -1 with correlation coefficient of 0.9979 and was precise at the intra-day level as reflected by relative standard deviation, accurate at recovery rate 99.75±0.08 and robust to change mobile phase and column brand. The detection and quantitation limits were 0.2956 μg.mL -1 and 0.5874 μg.mL -1 , respectively. The proposed method could be advantageous in estimation of melphalan quantitation in lyophilized nanosuspension form in the presence of excipients. Conclusion: The method was found to be simple, specific, rapid, precise, accurate and reproducible. The method was successfully applied for determination of the entrapment efficiency of melphalan from lyophilized nanosuspension and was found to 93.56 ± 4.32%.

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Section: Linearitymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Development and Validation of an HPLC- UV Method for the Determination of Melphalan from Lyophilized Nanosuspension

Ashok¹,

Ananda²

2019

IJPER

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Anatase titanium oxide nanoparticles and multi-walled carbon nanotubes-modified carbon paste electrode for simultaneous determination of avanafil and doxorubicin in plasma samples

Ali

Rageh

Abdel‐aal

et al. 2023

Microchemical Journal

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Quantitative analysis of doxorubicin hydrochloride and arterolane maleate by mid IR spectroscopy using transmission and reflectance modes

2021

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Background Environment-friendly fast and accurate mid-infrared spectroscopic methods have been developed for the quantitative analysis of doxorubicin hydrochloride (DOX) and arterolane maleate (ALM) in bulk and marketed formulations. Both transmittance and reflectance modes have been used for the analysis and a comparison has been drawn for better accuracy. The analytical methods were validated in accordance with International Council for Harmonisation (ICH) guidelines Results The proposed methods have been successfully developed and validated for the quantification of doxorubicin and arterolane maleate in solid bulk and dosage form. High recovery values in both the modes, while analysing DOX and ALM, indicated good accuracy of the methods. The methods showed excellent repeatability and intermediate precision [% RSD (Relative Standard Deviation < 2.0%]. The assay values of the drugs in solid dosage forms were also found close to the labelled claim. Conclusion The proposed Fourier transform infrared (FT-IR) spectroscopic methods were found to be specific, reproducible, valid and could be used as general methods for the quantification of most of the solid drug preparations such as tablets, capsules and powders.

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Development and Validation of RP-HPLC Method for Determination of Doxorubicin Hydrochloride from Vacuum Foam Dried Formulation

Cited by 10 publications

References 0 publications

Development and Validation of an HPLC- UV Method for the Determination of Melphalan from Lyophilized Nanosuspension

Development and Validation of an HPLC- UV Method for the Determination of Melphalan from Lyophilized Nanosuspension

Anatase titanium oxide nanoparticles and multi-walled carbon nanotubes-modified carbon paste electrode for simultaneous determination of avanafil and doxorubicin in plasma samples

Quantitative analysis of doxorubicin hydrochloride and arterolane maleate by mid IR spectroscopy using transmission and reflectance modes

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