Development and validation of RP-HPLC method for estimation of Cefotaxime sodium in bulk and formulation

Anwer, Esra T.; Porwal, Omji; Dudhe, Rupesh

doi:10.52711/0974-360x.2022.00521

Cited by 6 publications

(3 citation statements)

References 24 publications

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“…It was filtered through 0.45 lm nylon membrane filter and degassed. It was used as a diluent for the preparation of sample and standard solution [3].…”

Section: Buffer Preparationmentioning

confidence: 99%

Development and Validation of RP-HPLC method for the estimation of Plecanatide from Bulk and Pharmaceutical Dosage form

Deepika,

et al. 2023

World Journal of Current Med and Pharm Research

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A novel, simple, efficient, rapid, and precise reverse-phase high-performance liquid chromatography (RP-HPLC) method was developed for the estimation of Plecanatide in the bulk and pharmaceutical dosage form. The currently developed method was subsequently validated according to ICH guidelines in terms of linearity, accuracy, precision, the limit of detection, the limit of quantification, robustness, etc. The separation of the selected drugs was optimized after several trials including change of mobile phase and its composition, stationary phase, flow rate, column temperature, etc. The separation was performed by using an Develosil ODS HG-5 and UV absorption was measured at 246 nm. Ortho phosphoric acid, Triethylamine buffer : Methanol [520 : 480] was selected as the mobile phase at a flow rate of 1 mL/min. As per International Conference on Harmonization (ICH) Q2 R1 guidelines, several validation parameters were evaluated which include specificity, linearity, precision, accuracy, the limit of detection (LOD), and the limit of quantitation (LOQ). The acceptable degree of linearity range was found to be 10 to 60 µg/mL. The percent recovery was found to be 98.55 to 101.88%. Hence, the proposed method is simple, selective, and specifically meets the requirements of ICH guidelines for the validation of the analytical method.

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“…It was filtered through 0.45 lm nylon membrane filter and degassed. It was used as a diluent for the preparation of sample and standard solution [3].…”

Section: Buffer Preparationmentioning

confidence: 99%

Development and Validation of RP-HPLC method for the estimation of Plecanatide from Bulk and Pharmaceutical Dosage form

Deepika,

et al. 2023

World Journal of Current Med and Pharm Research

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“…In the field of DOI: 10.1002/masy.202300133 pharmaceutical analysis, High-Performance Liquid Chromatography (HPLC) has emerged as a powerful technique for the separation, identification, and quantification of drug compounds due to its high sensitivity, selectivity, and reproducibility. [1] Cefuroxime axetil (CEF), a secondgeneration cephalosporin antibiotic, exhibits broad-spectrum activity against a wide range of Gram-positive and Gramnegative bacteria. It is widely used in the treatment of various bacterial infections.…”

Section: Introductionmentioning

confidence: 99%

“…In the field of pharmaceutical analysis, High‐Performance Liquid Chromatography (HPLC) has emerged as a powerful technique for the separation, identification, and quantification of drug compounds due to its high sensitivity, selectivity, and reproducibility. [ 1 ]…”

Section: Introductionmentioning

confidence: 99%

RP HPLC Enabled Novel Analytical Method for the Development and Quantification of Cefuroxime Axetil in its Bulk Drug and Pharmaceutical Formulations

Mishra,

Behera,

Kar

et al. 2024

Macromolecular Symposia

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Current investigations aim to develop and validate an accurate, simple, specific, and precise RP‐HPLC method for the detection of cefuroxime axetil, in bulk and tablet dosage forms. Despite extensive literature reviews, no method could be found for estimating the variables mentioned above. The chromatographic separation is achieved on Sunfire C18 column (250 mm × 4.6 mm, 5 µm) of HPLC (Agilent Tech 1100), with 5 µm mobile phase composed of methanol, orthophosphoric acid (0.05%) adjusted to a pH of 3.0 in water, 40:60% v/v triethylamine using isocratic mode of elution, at wavelength 278.0 nm using UV Spectrophotometer, temperature 25 °C, detection is done. The developed method is done as per ICH guidelines. A linear calibration curve is found to be linear in concentrations between 1 and 5 µg mL−1 (r2 = 0.9995). The percentage recoveries of cefuroxime axetil are found to be in the range of 95.68–96.98%. The percentage of relative standard deviations in intraday trials is found to be 0.61, 0.66, and 0.88, respectively, and in interday studies are 0.06, 0.16, and 0.29, respectively, demonstrating good accuracy and little variance. The proposed method is highly sensitive, robust, precise, accurate, and the use of green solvents makes this method safe and efficacious.

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Electrochemically synthesized nanostructured gold-bismuth as novel sensor for efficient determination of cefotaxime in aqueous media

Elrouby,

Ameer,

Mohamed

et al. 2024

Microchemical Journal

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Development and validation of RP-HPLC method for estimation of Cefotaxime sodium in bulk and formulation

Cited by 6 publications

References 24 publications

Development and Validation of RP-HPLC method for the estimation of Plecanatide from Bulk and Pharmaceutical Dosage form

Development and Validation of RP-HPLC method for the estimation of Plecanatide from Bulk and Pharmaceutical Dosage form

RP HPLC Enabled Novel Analytical Method for the Development and Quantification of Cefuroxime Axetil in its Bulk Drug and Pharmaceutical Formulations

Electrochemically synthesized nanostructured gold-bismuth as novel sensor for efficient determination of cefotaxime in aqueous media

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