Development and validation of short-amplicon length PCR assay for macaques meat detection under complex matrices

Ali, Md. Eaqub; Rashid, Nur Raifana Abdul; Hamid, Sharifah Bee Abd; Hossain, Sakiat; Asing, Asing; Hossain, M. A. Motalib; Zaidul, I.S.M.

doi:10.1080/10942912.2016.1154573

Cited by 11 publications

(10 citation statements)

References 42 publications

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“…Results of this study suggest that the value allocated to baboons by park-adjacent dwellers is related mainly to consumptive uses. Primate meat consumption has been reported previously in many countries (Ali et al 2017;Blair et al 2017;De Thoisy et al 2005;Shaffer et al 2018;Tshikung et al 2019). In southeastern DRC for example, identified a notable quantity of baboon carcasses from parks and natural reserves illegally sold in urban markets of Lubumbashi, a city located nearly 150 km from the study area.…”

Section: Discussionmentioning

confidence: 71%

Ethnozoological uses and local people’s perceptions of a competitor Primate in the Fringe of the Kundelungu National Park, D.R. Congo

Kazaba¹,

Tinaye²,

Tshikung³

et al. 2020

Ethnobio Conserv

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Several studies investigate the human dimension of human-wildlife conflicts, but human attitudes towards forest-dependent animals such as primates in the context of competition for forest resources are still under-researched. We used a semi-structured questionnaire and conducted ethnozoological surveys in order to identify the uses of Kinda baboons (Papio kindae Lönnberg) and the main factors associated with local people's attitudes towards this frugivorous primate, at the periphery of the Kundelungu National Park, Democratic Republic of Congo. This study indicates that Kinda baboons are important to community members, essentially for consumptive uses, namely as bushmeat and medicinal animals. The association between local peoples' anti-conservation attitudes towards these baboons and observation of the depletion of co-used indigenous trees has been confirmed statistically, suggesting the perception of baboons as competitors. Also, even though the majority of respondents recognized the vulnerability of baboons to the depletion of co-used indigenous trees, against our hypothesis, most of them were not inclined to use these resources sustainably. However, an association between the frequency of encounters with Kinda baboons and park-adjacent dwellers' willingness to conserve baboon-edible trees has been established. We therefore support the view that improving the availability of co-used trees through reforestation or agroforestry is likely to alleviate the prevalence of negative attitudes towards tree-dependent animals. Also, in order to gain community support to protected areas and wildlife, pro-conservation campaigns in similar settings should not merely highlight the vulnerability of animals to the depletion of their resources, but also promote the responsible access of local people to protected areas.

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Section: Discussionmentioning

confidence: 71%

Ethnozoological uses and local people’s perceptions of a competitor Primate in the Fringe of the Kundelungu National Park, D.R. Congo

Kazaba¹,

Tinaye²,

Tshikung³

et al. 2020

Ethnobio Conserv

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“…The acceptability and value of buffalo meat vary culturally and geographically. In Europe and China, where it is widely accepted and eaten, it has a higher value than beef and is considered a delicacy (Hossain, Ali, Hamid, Hossain, et al, 2017;Wang, Hang, et al, 2019). In India, however, buffalo meat is less valued and is considered an adulterant of more valuable meat such as mutton (Naveena et al, 2017).…”

Section: Buffalomentioning

confidence: 99%

“…Products from PCR amplifications are subjected to enzymatic restriction (cutting) by specific endonucleases, resulting in a specific fingerprint in the product that is then used to identify the target species. Publicly available web domains provide online tools for researchers to perform in silico analyses to determine species-specific endonuclease and endonuclease sites for their targets (Ali et al, 2017). To detect Malaysian box turtle in food and traditional Chinese medicines, Asing et al (2016b) used the PCR-RFLP technique to restrict the 120 base pair (bp) long product of the cyt b gene with the Bfa1 endonuclease.…”

Section: Polymerase Chain Reaction-restriction Fragment Length Polymo...mentioning

confidence: 99%

A systematic review of DNA‐based methods in authentication of game and less common meat species

Adenuga

Montowska

2023

Comp Rev Food Sci Food Safe

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Despite the numerous studies on food safety and authenticity, especially for meat and meat products, not enough studies have been conducted focusing exclusively on game species and other unusual meat animals. As a result of the European horse scandal, the horse is currently the target of many meat authenticity studies. With this review, we aim to present various DNA-based methods that have been used by researchers to identify, detect, and quantify game, uncommon meat animals, and wildlife species. Polymerase chain reaction (PCR) is considered the standard method for DNA analysis in meat authenticity testing. However, in this paper, we present several other methods that may or may not involve the PCR technique. For this purpose, we systematically reviewed 131 articles selected according to various criteria such as target animal species, method of analysis, year of publication, and so forth. The result of our study shows the most studied game and uncommon meat species, PCR-and non-PCR-based methods for game meat analysis, and DNA-based methods in wildlife conservation. With this study, researchers can find detailed information about frequent game species used as adulterants for regular meat products and the DNA-based techniques to identify them.

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“…There is also a concern about PCR product carryover contamination due to the 10 8 -fold amplification of the target [26]. Finally, shorter DNA targets would be thermodynamically more stable than longer ones [27] and choosing a shorter target is favourable since it can withstand decomposition states [28].…”

Section: Introductionmentioning

confidence: 99%

Rapid and sensitive detection of box turtles using an electrochemical DNA biosensor based on a gold/graphene nanocomposite

Hashem¹,

Hossain²,

Marlinda³

et al. 2022

Beilstein J. Nanotechnol.

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The Southeast Asian box turtle, Cuora amboinensis, is an ecologically important endangered species which needs an onsite monitoring device to protect it from extinction. An electrochemical DNA biosensor was developed to detect the C. amboinensis mitochondrial cytochrome b gene based on an in silico designed probe using bioinformatics tools, and it was also validated in wet-lab experiments. As a detection platform, a screen-printed carbon electrode (SPCE) enhanced with a nanocomposite containing gold nanoparticles and graphene was used. The morphology of the nanoparticles was analysed by field-emission scanning electron microscopy and structural characteristics were analysed by using energy-dispersive X-ray, UV–vis, and Fourier-transform infrared spectroscopy. The electrochemical characteristics of the modified electrodes were studied by cyclic voltammetry, differential pulse voltammetry (DPV), and electrochemical impedance spectroscopy. The thiol-modified synthetic DNA probe was immobilised on modified SPCEs to facilitate hybridisation with the reverse complementary DNA. The turtle DNA was distinguished based on hybridisation-induced electrochemical change in the presence of methylene blue compared to their mismatches, noncomplementary, and nontarget species DNA measured by DPV. The developed biosensor exhibited a selective response towards reverse complementary DNAs and was able to discriminate turtles from other species. The modified electrode displayed good linearity for reverse complementary DNAs in the range of 1 × 10−11–5 × 10−6 M with a limit of detection of 0.85 × 10−12 M. This indicates that the proposed biosensor has the potential to be applied for the detection of real turtle species.

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Development and validation of short-amplicon length PCR assay for macaques meat detection under complex matrices

Cited by 11 publications

References 42 publications

Ethnozoological uses and local people’s perceptions of a competitor Primate in the Fringe of the Kundelungu National Park, D.R. Congo

Ethnozoological uses and local people’s perceptions of a competitor Primate in the Fringe of the Kundelungu National Park, D.R. Congo

A systematic review of DNA‐based methods in authentication of game and less common meat species

Rapid and sensitive detection of box turtles using an electrochemical DNA biosensor based on a gold/graphene nanocomposite

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