Development and validation of stability indicating RP-HPLC method for estimation of Perindopril Erbumine and Indapamide in bulk and pharmaceutical dosage form

Abstract

Background: Perindopril erbumine (PE) and Indapamide (IND) in combination were proven to have a synergistic antihypertensive impact when compared with the use of each component alone. Objectives: Therefore, a new simple, selective, precise, and stability indicating RP- HPLC method for analysis of Perindopril erbumine (PE) and Indapamide both in a bulk and in pharmaceutical formulation has been developed and validated. Methods: RP ‑ HPLC method, Younglin (S.K.) Gradient System UV Detector and C18 column with 250mm x 4.6 mm i. d. and 5μm particle size acetonitrile 70+ water with OPA 30 was used as the mobile phase for the method. The detection wavelength was 215 nm and flow rate was 1 ml/min. Results: In the developed method, the retention time of Perindopril erbumine (PE) and Indapamide were found to be 5.4833 min and 9.4500min. The developed method was validated according to the ICH guidelines. The linearity, precision, range, robustness was within the limits as specified by the ICH guidelines. Conclusion: Hence the method was found to be simple, accurate, precise, economic and reproducible. The forced degradation studies proved stability indicating power of the method.

Development and Evaluation of Trazodone Hydrochloride Tablets for Oral Drug Delivery Technology

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