Background: Perindopril erbumine (PE) and Indapamide (IND) in combination were proven to have a synergistic antihypertensive impact when compared with the use of each component alone. Objectives: Therefore, a new  simple,  selective, precise, and stability indicating RP- HPLC method for analysis of  Perindopril erbumine (PE) and Indapamide both in a bulk and in pharmaceutical formulation has been developed and validated. Methods: RP ‑ HPLC  method, Younglin (S.K.) Gradient System  UV Detector  and C18  column with 250mm  x 4.6 mm i. d. and 5μm particle size acetonitrile 70+  water with OPA 30 was used as the mobile phase for the method. The detection wavelength was 215 nm and flow rate was 1 ml/min. Results: In the developed method, the retention time of Perindopril erbumine (PE) and Indapamide were found to be 5.4833 min and 9.4500min. The developed method was validated according to the ICH guidelines. The linearity, precision, range, robustness was within the limits as specified by the ICH guidelines. Conclusion: Hence the method was found to be simple, accurate, precise, economic and reproducible. The forced degradation studies proved stability indicating power of the method.

This work aimed to create a speedy, precise, and selective HPTLC and RP-UHPLC method for analysing andrographolide in fi nished products and raw materials. Asian medicines are using Andrographis paniculata since long. It’s used to treat skin eruptions, boils, scabies, and chronic, unexplained fevers, all of which are caused by blood “abnormalities.” Liquid chromatographic methods were developed to separate andrographolide and its herbal dosage form. HPTLC chromatography employed a 10 by 10 cm aluminum plate coated with 0.2 mm of silica gel 60 F254 (E. Merck, Germany). Camag Linomat 5 applicator with 100 μL syringe was used to apply samples in 6 mm bands (Hamilton, Switzerland). 14 mm separated the two bands, and 150 nL sec-1 was applied. Mobile phase was dichloromethane, toluene, ethyl acetate, and formic acid (6:4:1:0.5). This chromatogram runs 80 mm. Camag TLC scanner measured density at 254 nm. Mean RF=0.69. The linear calibration curve covers 500–3000 ng/spot and has a 0.996 correlation coeffi cient. Limit of Detection: 31.5 ng; Limit of Quantitation: 95.48 ng. A validated RP-UHPLC method for quantifying andrographolide in extract and formulation has been established. UHPLC analysis was performed in isocratic mode, at room temperature, using acetonitrile: Water (0.2% acetic acid) (85:15, v/v) as mobile phase on a 250 mm 4.6 mm i.d., 5 μm Cosmosil C18 column. Detection was at 230 nm. Andrographolide has a 4.1 minute half-life. Between 10 and 60 g/mL, andrographolide was linear. The approach met or exceeded ICH’s linearity, precision, accuracy, and robustness

New medication combinations are introduced every day. As a result, various diseases and disorders are treated using a combination of several therapeutic medicines that each have a somewhat distinct mechanism of action. Therefore, it is crucial to develop methods of analyzing medicines employing a range of methods that may be utilized. A UV 730d (dad) absorbance detector, a 20 L injection loop, a sp 930d pump, a 4.6 by 100 mL C18 column (Agilent), and Chemstation software are all included in the setup: approximately 60 water and 40% methanol (pH 3.0 adjust with OPA). Maximum effi ciency was achieved when the system was operated at a wavelength of 233 nm. The procedure’s effi cacy was confi rmed by testing it against ICH guidelines. These techniques were found to be linear, precise, broad, and stable. The procedure was found to be easy, accurate, exact, aff ordable, and easy to use again and again. This means that olmesartan and hydrochlorothiazide, in both bulk form and fi nished products, can be tested for quality using the proposed methodologies.

The day-by-day new combinations drugs are being introduced in market. Then the multiple therapeutic agents which acts at different sites are used in the management of various diseases and disorders are done. Thus it is necessary to develop methods for analysis with the help of number of analytical techniques which are available for the estimation of the drugs in combination. An  accurate,  precise  and  reproducible  UV spectrophotometer method  was developed for the simultaneous quantitative determination of Sofosbuvir and Ledipasvir in tablet dosage forms. Methods: Younglin (S. K.) gradient system UV detector and C18 column with 250 mm x 4.6 mm i. d.  and 5μm particle size Acetonitrile: OPA water (80: 20v/v) pH 2.5 was used as the mobile phase for the method. The detection wavelength was 283 nm and flow rate was 0.9 ml/min. Results: In the developed method, the retention time of Sofosbuvir and Ledipasvir were found to be 6.366 min and 8.616 min. The developed method was validated according to the ICH guidelines. Conclusion: In this methods linearity, precision, range, robustness were observed. The method was found to be simple, accurate, precise, economic and reproducible.

Objective: The day by day new combinations drugs are being introduced in market. Then the multiple therapeutic agents which acts at different sites are used in the management of various diseases and disorders are done. Thus it is necessary to develop methods for analysis with the help of number of analytical techniques which are available for the estimation of the drugs in combination. An  accurate,  precise  and  reproducible  RP-HPLC  method  was developed for the simultaneous quantitative determination of METO and BENI in tablet dosage forms. Methods: Younglin (S. K.) gradient system UV detector and C18 column with 250 mm x 4.6 mm i. d.  and 5μm particle size Acetonitrile: OPA water (45: 55v/v) pH 2.5 was used as the mobile phase for the method. The detection wavelength was 230 nm and flow rate was 1ml/min. Results: In the developed method, the retention time of Metoprolol and Benidipine were found to be 2.9833 min and 7.3833 min. The developed method was validated according to the ICH guidelines. Conclusion: The developed method was validated according to the ICH guidelines. In this methods linearity, precision, range, robustness was within the limits as specified by the ICH guidelines.