Development and validation of the method for simultaneous determination of benzydamine hydrochloride and methylparaben in benzydamine dosage form by GC

Chornyi, Vasyl; Georgiyants, Victoriya

doi:10.14748/ssp.v6i1.6036

Cited by 4 publications

(3 citation statements)

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“…Thus, T-Sept ® has pronounced analgesic and anti-exudative properties, as well as an active anti-inflammatory effect. With local application in the form of periodontal dressing with a prolonged effect, benzydamine accumulates in inflamed tissues, where effective concentrations are precisely achieved due to its ability to penetrate through the mucous membrane [35,36].…”

Section: Discussionmentioning

confidence: 99%

Study of Cytocompatibility of Different Supplements in Gel Compositions Used for the Treatment of Periodontal Diseases in Orthodontic Patients via Exploration of Mechanisms of Their Cytopathic and Cytoprotective Effects in Cultured Human and Mouse Cells

Hodovanyi

Чухрай

Hodovana

et al. 2023

Proc Shevchenko Sci Soc Med Sci

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Introduction. A search continues for effective means which may reduce the overload of harmful factors, eliminate the inflammatory process, and reduce stress on the periodontal tissues during the active period of orthodontic treatment. We developed and patented the gel composition (GC) Benzidaflaziverdine prepared based on Proteflazid® (flavonoids) and benzydamine hydrochloride (BH) T-Sept® for the local treatment of the periodontal tissues in the form of a periodontal dressing in the orthodontic patients. The aim of this study was to evaluate the cytocompatibility of different combinations of components in gel composition based on flavonoid complex and benzydamine hydrochloride (Benzidaflaziverdine) used for the treatment of periodontal diseases in orthodontic patients. For this, mechanisms of their cytopathic and cytoprotective effects will be explored using cultured human and mouse cells. Methods. We studied the effect of different supplements used in GC Benzidaflaziverdine on the viability of pseudonormal human keratinocytes of the HaCaT line and mouse fibroblasts of the BALB-3T3 line, and mouse macrophages of the J774.2 line. Various methods of cell survival assessment were used: MTT-assay, staining of cells with fluorescent dyes Hoechst 33342 and Propidium iodide (PI), as well as a test for the genotoxic effects on cells (DNA comet assay). The antioxidant properties of the developed GC variants were evaluated using DPPH (1,1-diphenyl-2-picrylhydrazyl), Merck (Dam-stadt, Germany), and DCFDA-H2 (2’,7’-dichlorodihydrofluorescein diacetate). Results. We demonstrated that the Sample containing gel base and BH in the form of a solution (Tantum Verde®) possessed weak prooxidant properties. While the Sample contained gel base, powdered BH (T-Sept®) and Sample containing gel base and powdered BH (T-Sept® and Proteflazid®) possessed pronounced antioxidant properties. Conclusions. Tests with DPPH and DCFDA dyes were used to confirm the hypothesis regarding the cytoprotective effect of the patented gel composition Benzidaflaziverdine for local application in the form of a periodontal bandage due to the antioxidant activity of the flavonoid complex, which reaches the maximum level at the 2nd hour of exposure. This gel composition can be recommended for use in clinical periodontology for medical support of orthodontic patients before and during the active phase of orthodontic treatment.

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Section: Discussionmentioning

confidence: 99%

Study of Cytocompatibility of Different Supplements in Gel Compositions Used for the Treatment of Periodontal Diseases in Orthodontic Patients via Exploration of Mechanisms of Their Cytopathic and Cytoprotective Effects in Cultured Human and Mouse Cells

Hodovanyi

Чухрай

Hodovana

et al. 2023

Proc Shevchenko Sci Soc Med Sci

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“…Вибір ефективної схеми лікування захворювань пародонта повинен включати застосування етіологічних, патогенетичних та симптоматичним підходів з урахуванням сучасних концепцій етіології даної патології. Саме такий своєчасний підхід гарантуватиме якісний та довготривалий терапевтичний ефект [11][12][13].…”

Section: вступunclassified

“…Розчини для полоскання, які мають стійкий і тривалий терапевтичний ефект, повинні бути включені до схем лікування захворювань пародонта. Акцентувати увагу слід на медикаменти із нестероїдною протизапальною дією, серед яких особливо виділяється препарат на основі бензидаміну гідрохлориду, що наділений протизапальними, протиексудативними властивостями, а окрім цього здатен чинити і місцеву анальгезуючу дію [11,12]. Хлорофіліпт, у свою чергу, є сумішшю хлорофілів з листя евкаліпта.…”

Section: обговорення результатів дослідженняunclassified

Treatment Efficacy for Periodontal Diseases in Middle-Aged Individuals

Horban,

Pasichnyk,

Mykyyevych

et al. 2024

Act. Probl. of the Modern Med.

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The aim of the study is to evaluate and analyze the effectiveness of the proposed treatment mode for periodontal disease in middle-aged individuals. Subjects and methods.The study comprised 62 individuals aged 46 to 60 years, including 32 men and 30 women with periodontal tissue diseases. Among them there were 24 patients diagnosed with chronic catarrhal gingivitis, and 38 patients with generalized periodontitis at the initial-I degree of development. The examinations were conducted at the Dental Medical Centre of Danylo Halytskyi Lviv National Medical University. The comparison group underwent standard treatment, while the intervention group was recommended to use the "Forteza" rinse solution and "Chlorophyllipt" tablets. Various indices were evaluated in all patients, including the Green-Vermillion index, periodontal index, and papillary bleeding index, both before treatment, immediately after treatment, and 6 months post-treatment. Results. The results obtained indicated a significant decrease (p > 0.05) in hygiene, bleeding, and periodontal indices among patients with chronic catarrhal gingivitis after treatment in both groups. However, it was noted that the baseline group exhibited superior outcomes compared to the comparison group. This difference persisted even 6 months after treatment. Similar results were obtained in patients with generalised periodontitis of the initial-I degree: the studied indices decreased in all patients immediately after treatment, but in the baseline group, the indices were significantly better immediately after treatment, and after six months. Conclusions. The study confirmed significantly better treatment results in the baseline group. In particular, in patients with CСG, the PBI index and PI index values in six months after the treatment completion were significantly better in group I B in 1.6 times (p>0.05) than in group I A. A similar result was obtained in patients with initial, I degree, GP, namely, in group II B, the PBI was significantly better in 1.6 times (p>0.05) than in group II A, and the PI index was 1.4 times, respectively. Therefore, the prospect of further research is the implementation of an effective treatment mode for periodontal disease in the clinical practice for various age groups.

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Finger pump microfluidic detection system for methylparaben detection in foods

Liu

Huang

et al. 2023

Food Chemistry

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Development and validation of the method for simultaneous determination of benzydamine hydrochloride and methylparaben in benzydamine dosage form by GC

Cited by 4 publications

References 0 publications

Study of Cytocompatibility of Different Supplements in Gel Compositions Used for the Treatment of Periodontal Diseases in Orthodontic Patients via Exploration of Mechanisms of Their Cytopathic and Cytoprotective Effects in Cultured Human and Mouse Cells

Study of Cytocompatibility of Different Supplements in Gel Compositions Used for the Treatment of Periodontal Diseases in Orthodontic Patients via Exploration of Mechanisms of Their Cytopathic and Cytoprotective Effects in Cultured Human and Mouse Cells

Treatment Efficacy for Periodontal Diseases in Middle-Aged Individuals

Finger pump microfluidic detection system for methylparaben detection in foods

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