Development and Validation of Uv Spectrophotometric Method for Determination of Ceftazidime in Bulk and Pharmaceutical Formulation

Abstract

The present study was undertaken to develop and validate a simple, accurate, precise, reproducible and cost effective UV-Visible spectrophotometric method for the estimation of ceftazidime in bulk and pharmaceutical formulation. The solvent used throughout the experiment was the mixer of methanol and water. Absorption maximum (λmax) of the drug was found to be 266 nm. The quantitative determination of the drug was carried out at 266 nm and Beer's law was obeyed in the range of 2-20 μg/mL. The method was shown linear in the mentioned concentrations having line equation y = 0.044x with correlation coefficient of 0.999. The recovery values for ceftazidime ranged from 99.83 % -100.56 %. The relative standard deviation of six replicates of assay was less than 2 %. The percent relative standard deviation (RSD %) of interday precision range was 0.275 -0.420 % and intraday precision range was 0.222 -0.418 %. The limit of detection and limit of quantification was 0.079 μg/mL and 0.241 μg/mL. The percent relative standard deviation of robustness and ruggedness of the method was 0.146 -0.231 %. Hence, proposed method was precise, accurate and cost effective. This method could be applicable for quantitative determination of the bulk drug as well as dosage formulation.

Developing a highly validated and sensitive HPLC method for simultaneous estimation of cefotaxime and paracetamol in pure and pharmaceutical preparations

“…1 The literature review revealed that several analytical approaches have been utilised in the determination of cefotaxime such as HPLC, UV-VIS spectrophotometric, potientiometry, electrophoresis and hydrophilic interaction chromatography. [2][3][4][5][6][7][8][9] Paracetamol (PAR); N-(4-Hydroxyphenyl) acetamide (Fig. 1) is related to a non-steroidal anti-inflammatory drugs (NSAIDs) which acts centrally and peripherally for treatment of non-inflammatory conditions.…”

Development and Validation of a UV/vis Spectrometric Method for Determination of Cefixim Trihydrate in Raw Materials (Pure) and Tablet Forms

“…To overcome this difficulty, Babitan et al as well as Umme Bushra et al have each developed and validated a method for analyzing Cefixim using a non-separating technique which is Ultraviolet-visible spectrophotometry using methanol as a solvent [4]. A part from these previous researches, another Spectrometric Method using Methanol and water as solvent was developed, to deal with analysis of Cefixim in 287 nm as maximal wavelength to the measurement [5]. The method is simple and less expensive than Liquid Chromatography but requires the use of organic solvent which costs more.…”