Development and validation of UV-spectrophotometric methods for quantitative estimation of Prothionamide in pure and pharmaceutical dosage forms

Abstract

UV Spectrophotometric method was developed and validated for the quantitative determination of Prothionamide in bulk drug and in pharmaceutical formulations. Prothionamide shows the maximum absorbance at 288 nm in phosphate buffer (pH 7.4). Prothionamide follows Beer’s law in the concentration range of 4-20 µg/ml (r2 = 0.999). The detection limit (DL) and quantitation limit (QL) were 0.406 and 1.229 µg/ml respectively. Accuracy and precision were found to be satisfactory. The developed methods were validated according to ICH guidelines. All the validation parameters were found to be satisfactory accordance with the standard values. Therefore, the proposed method can be used for routine practice for the determination of Prothionamide in assay of bulk drug and pharmaceutical formulations.International Current Pharmaceutical Journal, June 2015, 4(7): 402-404

Development and evaluation of Chitosan nanoparticles based dry powder inhalation formulations of Prothionamide

“…Percentage Drug Entrapment (PDE) of PTH in nanoparticles was determined by separating the nanoparticles from the aqueous medium by ultracentrifugation at 31150g for 30 min. The amount of free PTH in the supernatant was measured by UV-Spectrophotometery at 288nm [ 15 ]. Other evaluation parameters were calculated as reported previously [ 16 ].…”

Development of Dry Powder Inhaler Containing Prothionamide-PLGA Nanoparticles Optimized Through Statistical Design: In-vivo Study

“…After centrifugation, un-entrapped PTH in the supernatant was estimated by UV-spectrophotometric method as reported previously [15]. Entrapped drug was calculated by subtracting un-entrapped drug from total drug.…”

“…These vials were kept in stability chamber and maintained at 25±2°C & 60±5% RH. The nanoparticles were analyzed for the period of 6 month [15,21,22]. Zeta size, zeta potential, PDI, drug entrapment and drug release were carried out to check the stability of dry powder inhaler of PTH nanoparticles.…”

Graphene oxide-based electrochemical activation of ethionamide towards enhanced biological activity

“…8 Considering the above mentioned side effects, researchers have developed various analytical tools for the determination and quantication of ETO from various formulations and even real samples. [9][10][11][12][13][14][15] For example, Sujitkumar et al reported a UV-Vis spectrophotometric method for ETO estimation from bulk and tablets. 10 Madni et al gave an account of an HPLC method for the determination of ETO from serum.…”

“…[9][10][11][12][13][14][15] For example, Sujitkumar et al reported a UV-Vis spectrophotometric method for ETO estimation from bulk and tablets. 10 Madni et al gave an account of an HPLC method for the determination of ETO from serum. 11 Moreover, the spectrometric estimation of ETO was carried out using Folin-Ciocalteu reagent with iron(III)-ferricyanide as the chromogenic agent complexing with ETO.…”