2002
DOI: 10.1046/j.1439-0523.2002.00656.x
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Development and variability analysis of microsatellite markers in peach

Abstract: A genomic DNA library enriched with AG/CT repeats has been developed from the peach cultivar ‘Merrill O'Henry’. The enrichment method was efficient, with 61% of the clones obtained carrying a microsatellite sequence and a yield of one polymorphic microsatellite every 2.17 sequenced clones. From 35 microsatellites detected, 24 were polymorphic in a set of 25 cultivars including 14 peaches and 11 nectarines. A total of 82 alleles were found with the polymorphic microsatellites, with an average of a 37% of observ… Show more

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Cited by 223 publications
(126 citation statements)
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“…However, microsatellites or simple sequence repeats (SSRs) have become the markers of choice for fingerprinting and paternity analyses in most plant species (Gupta & Varshney, 2000) due to their high polymorphism, codominancy and reproducibility. Over 100 microsatellite markers have been so far reported in peach (Cipriani et al, 1999;Testolin et al, 2000;Sosinski et al, 2000;Aranzana et al, 2002Aranzana et al, , 2003Dirlewanger et al, 2002) and many of them are transferable to other related species (Hormaza, 2002;Wünsch & Hormaza, 2002).…”
Section: Introductionmentioning
confidence: 99%
“…However, microsatellites or simple sequence repeats (SSRs) have become the markers of choice for fingerprinting and paternity analyses in most plant species (Gupta & Varshney, 2000) due to their high polymorphism, codominancy and reproducibility. Over 100 microsatellite markers have been so far reported in peach (Cipriani et al, 1999;Testolin et al, 2000;Sosinski et al, 2000;Aranzana et al, 2002Aranzana et al, , 2003Dirlewanger et al, 2002) and many of them are transferable to other related species (Hormaza, 2002;Wünsch & Hormaza, 2002).…”
Section: Introductionmentioning
confidence: 99%
“…Given their high polymorphism, codominant inheritance, and the simplicity of the methods used to develop them, microsatellites, or simple sequence repeat (SSR), appeared as appropriate markers (Morgante and Olivieri, 1993). Many of these were developed in peach (Cipriani et al, 1999;Sosinski et al, 2000;Testolin et al, 2000;Aranzana et al, 2002;Dirlewanger et al, 2002) and cherry (Downey and Iezzoni, 2000;Cantini et al, 2001). By collecting all the available information, Aranzana et al (2003b) analyzed 109 SSRs developed in peach and cherry by different research groups and mapped 96 of them on the Prunus reference map (Joobeur et al, 1998).…”
Section: Construction Of Genetic Mapsmentioning
confidence: 99%
“…Microsatellite or Simple Sequence Repeats (SSRs) are the best available choice of markers for peach genetics and breeding, they are generally codominant, highly polymorphic, and can be found in large numbers covering the whole genome of any species (Sosinski et al, 2000). Many SSRs have been developed in peach (Testolin et al, 2000;Aranzana et al, 2002;Dirlewanger et al, 2002). The objectives of this study were to:…”
Section: Fruit Breedingmentioning
confidence: 99%