Development, characterization and in vitro toxicity evaluation of nanoemulsion-loaded hydrogel based on copaiba oil and coenzyme Q10

Nigro, Fiammetta; Cerqueira, Cristal; Rossi, André L.; Cardoso, Verônica da Silva; Vermelho, Alane Beatriz; Ricci-Júnior, Eduardo; Santos, Elisabete Pereira dos; Mansur, Cláudia R. E.

doi:10.1016/j.colsurfa.2019.124132

Cited by 14 publications

(6 citation statements)

References 39 publications

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“…These results highlight how copaiba oil may interact with cells by in vitro assessments, which could lead not only to different toxicological profiles, but also to the safe concentrations that can be used in phytotherapeutic medicines development [ 23 ]. Moreover, Nigro et al (2020) evaluated the cytotoxicity of copaiba oil nanoemulsion on fibroblasts and keratinocytes using the MTT assay and found a concentration-dependent mitochondria enzyme activity reduction effect (above 30%) from 300 μg·mL −1 to 10 mg.mL −1 in both cell types, which is similar to our results with CopNc [ 53 ].…”

Section: Resultssupporting

confidence: 89%

Copaiba Oil-Loaded Polymeric Nanocapsules: Production and In Vitro Biosafety Evaluation on Lung Cells as a Pre-Formulation Step to Produce Phytotherapeutic Medicine

et al. 2023

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Copaiba oil has been largely used due to its therapeutic properties. Nanocapsules were revealed to be a great nanosystem to carry natural oils due to their ability to improve the bioaccessibility and the bioavailability of lipophilic compounds. The aim of this study was to produce and characterize copaiba oil nanocapsules (CopNc) and to evaluate their hemocompatibility, cytotoxicity, and genotoxicity. Copaiba oil was chemically characterized by GC-MS and FTIR. CopNc was produced using the nanoprecipitation method. The physicochemical stability, toxicity, and biocompatibility of the systems, in vitro, were then evaluated. Β-bisabolene, cis-α-bergamotene, caryophyllene, and caryophyllene oxide were identified as the major copaiba oil components. CopNc showed a particle size of 215 ± 10 nm, a polydispersity index of 0.15 ± 0.01, and a zeta potential of −18 ± 1. These parameters remained unchanged over 30 days at 25 ± 2 °C. The encapsulation efficiency of CopNc was 54 ± 2%. CopNc neither induced hemolysis in erythrocytes, nor cytotoxic and genotoxic in lung cells at the range of concentrations from 50 to 200 μg·mL−1. In conclusion, CopNc showed suitable stability and physicochemical properties. Moreover, this formulation presented a remarkable safety profile on lung cells. These results may pave the way to further use CopNc for the development of phytotherapeutic medicine intended for pulmonary delivery of copaiba oil.

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Section: Resultssupporting

confidence: 89%

Copaiba Oil-Loaded Polymeric Nanocapsules: Production and In Vitro Biosafety Evaluation on Lung Cells as a Pre-Formulation Step to Produce Phytotherapeutic Medicine

et al. 2023

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“…Photosensitizers immobilized within a polymer matrix were used to obtain and utilize an antimicrobial photoactive material. As a polymer, the ammonium acryloyldimethyltaurate copolymer (AVC), being a rheology modifier widely used in pharmacology and dermatology, was applied [ 34 , 35 , 36 ]. The modifier is a copolymer that consists of acrylamido-2,2-dimethylpropanesulfonic acid and vinylpyrrolidone, with an alternating arrangement within the macromolecule.…”

Section: Introductionmentioning

confidence: 99%

Bacteria Single-Cell and Photosensitizer Interaction Revealed by Quantitative Phase Imaging

Buzalewicz

Ulatowska-Jarża

Kaczorowska

et al. 2021

IJMS

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Quantifying changes in bacteria cells in the presence of antibacterial treatment is one of the main challenges facing contemporary medicine; it is a challenge that is relevant for tackling issues pertaining to bacterial biofilm formation that substantially decreases susceptibility to biocidal agents. Three-dimensional label-free imaging and quantitative analysis of bacteria–photosensitizer interactions, crucial for antimicrobial photodynamic therapy, is still limited due to the use of conventional imaging techniques. We present a new method for investigating the alterations in living cells and quantitatively analyzing the process of bacteria photodynamic inactivation. Digital holographic tomography (DHT) was used for in situ examination of the response of Escherichia coli and Staphylococcus aureus to the accumulation of the photosensitizers immobilized in the copolymer revealed by the changes in the 3D refractive index distributions of single cells. Obtained results were confirmed by confocal microscopy and statistical analysis. We demonstrated that DHT enables real-time characterization of the subcellular structures, the biophysical processes, and the induced local changes of the intracellular density in a label-free manner and at sub-micrometer spatial resolution.

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“…In fact, a stable, skin-compatible pH of the optimized Ac PE nano cream (pH < 5) is crucial for alleviating the discomfort of skin problems such as dermatitis or impaired skin barrier [ 27 , 28 ]. Also, Nigro et al [ 29 ] and Roselan et al [ 30 ] documented the same outcome in their copaiba oil and kojic monooleate nanoemulsions, registering the corresponding pH values of 4.60 and 5.75. Hence, the findings of this study supported the adequacy of the optimal nanocream as a topical application on the human skin.…”

Section: Resultsmentioning

confidence: 58%

Ananas comosus Peels Extract as a New Natural Cosmetic Ingredient: Oil-in-Water (O/W) Topical Nano Cream Stability and Safety Evaluation

Yahya

Wahab

Attan

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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Ananas comosus peels (AcP) are among the agro-industrial biomasses contributing to a significant volume of waste in Malaysia. Thus, the AcP extract (AcPE) may prove useful for other applications, such as an ingredient in a nanocream for controlled delivery for dermal application. Therefore, this study aimed to develop an oil-in-water (O/W) nanocream using ingredients derived from the AcPE and test its stability alongside safety evaluation. The extract is a rich source of polyphenolic compounds viz., catechin, quercetin, and gallic acid. The study discovered that the optimized AcPE nano cream was stable against coalescence during the accelerated test but was influenced by Ostwald ripening over 6 weeks of storage at 4°C. Safety assessments affirmed the AcPE nano cream to be free of microbial contamination and heavy metals. The findings conveyed that the A. comosus nano cream is a good cosmetic ingredient and may contribute to the cosmeceutical industry’s new and safe topical products.

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Development, characterization and in vitro toxicity evaluation of nanoemulsion-loaded hydrogel based on copaiba oil and coenzyme Q10

Cited by 14 publications

References 39 publications

Copaiba Oil-Loaded Polymeric Nanocapsules: Production and In Vitro Biosafety Evaluation on Lung Cells as a Pre-Formulation Step to Produce Phytotherapeutic Medicine

Copaiba Oil-Loaded Polymeric Nanocapsules: Production and In Vitro Biosafety Evaluation on Lung Cells as a Pre-Formulation Step to Produce Phytotherapeutic Medicine

Bacteria Single-Cell and Photosensitizer Interaction Revealed by Quantitative Phase Imaging

Ananas comosus Peels Extract as a New Natural Cosmetic Ingredient: Oil-in-Water (O/W) Topical Nano Cream Stability and Safety Evaluation

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