Development of 21 microsatellite loci for puma (<i>Puma concolor</i>) ecology and forensics

Kurushima, Jennifer D.; Collins, Julia A.; Well, Jay A.; Ernest, Holly B.

doi:10.1111/j.1471-8286.2006.01508.x

Cited by 27 publications

(24 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We optimized conditions for microsatellite DNA primers, six of which were developed from the domestic cat (Felis catus) genome (Culver et al 2000;Menotti-Raymond et al 2005) and six developed from the mountain lion genome (Kurushima et al 2006;Rodzen et al 2007). The six F. catus loci used were Fca-43, Fca-57, Fca-82, Fca-90, Fca-96, and Fca-166.…”

Section: Pcr Amplification and Genotypingmentioning

confidence: 99%

“…The six P. concolor loci used were Pco-A2, Pco-B105, Pco-B010, Pco-D8, Pco-D301, and Pco-D329. The universal M13 primer was added at the 5′ end of the forward primers to allow fluorescent labeling of the amplicons (Schuelke 2000;Kurushima et al 2006), and reverse primers were designed with a "pig-tail" at the 5′ end to reduce variability in adenylation of amplification products (Brownstein et al 1996). Conditions for PCR amplification were 1× PCR buffer, 0.2 μM each dNTP, 0.05% bovine serum albumin, 0.1 μM M13 universal labeled primer, 0.01 μM M13-tailed forward primer, 0.1 μM pig-tailed reverse primer, and 0.25 U of Taq Polymerase enzyme (Qiagen), in a total volume of 10 μl.…”

Section: Pcr Amplification and Genotypingmentioning

confidence: 99%

See 1 more Smart Citation

Spatial and temporal interactions of sympatric mountain lions in Arizona

et al. 2011

View full text Add to dashboard Cite

Spatial and temporal interactions among individual members of populations can have direct applications to habitat management of mountain lions (Puma concolor). Our objectives were to evaluate home range overlap and spatial/ temporal use of overlap zones (OZ) of mountain lions in Arizona. We incorporated spatial data with genetic analyses to assess relatedness between mountain lions with overlapping home ranges. We recorded the space use patterns of 29 radio-collared mountain lions in Arizona from August 2005 to August 2008. We genotyped 28 mountain lions and estimated the degree of relatedness among individuals. For 26 pairs of temporally overlapping mountain lions, 18 overlapped spatially and temporally and eight had corresponding genetic information. Home range overlap ranged from 1.18% to 46.38% (x¼24:43, SE=2.96). Male-male pairs were located within 1 km of each other on average, 0.04% of the time, whereas male-female pairs on average were 3.0%. Two male-male pairs exhibited symmetrical spatial avoidance and two symmetrical spatial attractions to the OZ. We observed simultaneous temporal attraction in three male-male pairs and four male-female pairs. Individuals from Tucson were slightly related to one another within the population (n=13, mean R=0.0373 ±0.0151) whereas lions from Payson (n=6, mean R = −0.0079 ± 0.0356) and Prescott (n =9, mean R=−0.0242±0.0452) were not as related. Overall, males were less related to other males (n=20, mean R=−0.0495± 0.0161) than females were related to other females (n=8, mean R=0.0015±0.0839). Genetic distance was positively correlated with geographic distance (r 2 =0.22, P=0.001). Spatial requirements and interactions influence social behavior and can play a role in determining population density.

show abstract

Section: Pcr Amplification and Genotypingmentioning

confidence: 99%

Section: Pcr Amplification and Genotypingmentioning

confidence: 99%

Spatial and temporal interactions of sympatric mountain lions in Arizona

et al. 2011

View full text Add to dashboard Cite

show abstract

“…To individualize each fecal sample, we amplified seven microsatellites loci (PcoA208, PcoC108, PcoB003, PcoB010, PcoB210, PcoA339 and PcoA216) described by Kurushima et al [31], following the PCR protocol described in Miotto et al [17]. The PCR products were genotyped in MegaBACE ET-550R Size Standard sequencer (Amersham Biosciences, Little Chalfont, UK).…”

Section: Microsatellite Genotyping Fecal Samples Individualization mentioning

confidence: 99%

Evidence of Recent Fine-Scale Population Structuring in South American Puma concolor

Saranholi¹,

Chávez-Congrains²,

Galetti³

2017

Diversity

View full text Add to dashboard Cite

Habitat loss and fragmentation are considered the major treats to worldwide biodiversity. Carnivores in particular can be more sensitive to landscape modification due to their low occurrence density and large home ranges. Population structuring of Puma concolor has been already reported as a consequence of extensive human activities in the North American continent. Here, we investigated the occurrence of fine-scale population structuring in the South American cougar population, contrasting two conservation areas immersed in a highly human-fragmented landscape dominated by the presence of sugar cane monoculture, roads, and urbanization, including a series of dams in the Tietê River which enlarges its water body. Seven microsatellites were amplified using non-invasive DNA obtained from fecal samples. We conducted genetic clustering analyses using Bayesian and factorial components. We also performed genetic differentiation analyses by fixation indices (F st and D est ). Two genetic clusters represented by individuals from each area were found, indicating the occurrence of gene flow reduction between the areas. The intense human-induced landscape modification-which includes the Tietê River water body enlargement, imposing physical barriers to the movement of the individuals-could explain the gene flow reduction. Increasing connectivity among the preserved areas can mitigate such effects, and the creation of corridors or further management actions such as individual translocation to ensure gene flow in the highly-modified landscape may be essential for maintaining the genetic and demographic health of the species and its long-term persistence.

show abstract

“…To individualize each fecal sample, we amplified a set of 12 species-specific microsatellite loci with primers developed by Kurushima et al (2006): Pco C209, Pco D217, Pco D103, Pco C217, Pco C112, Pco C108, all tetranucleotide-repeat microsatellite loci; and Pco B010, Pco B210, Pco A339, Pco A208, Pco A216, Pco B003, dinucleotide-repeat microsatellite loci. Primers were marked with universal fluorescent M13 tails according to Schuelke (2000).…”

Section: Fecal Samples Individualization and Genetic Analysismentioning

confidence: 99%

Genetic diversity and population structure of pumas (Puma concolor) in southeastern Brazil: implications for conservation in a human-dominated landscape

et al. 2011

View full text Add to dashboard Cite

São Paulo is the most populous, developed and industrialized state of Brazil. Despite of intensive human activities, large habitat loss and fragmentation of the native vegetation cover, pumas (Puma concolor) still inhabit remnant habitat fragments in the northeastern area of the state. We investigated the occurrence of genetic structure and levels of genetic variability on pumas to aggregate basic information for conservation efforts to maintain long term viable populations of this top-predator in the region. By analyzing microsatellite loci variation, we corroborated the hypothesis of absence of genetic structure, and estimated high levels of genetic diversity (expected heterozygosity of 0.79 and mean of 10 alleles per locus). In spite of the increasing number of roadkilling and puma-human conflicts in the area, apparently pumas still maintain some level of gene flow between protected areas of the region. The observed excess of heterozygotes suggests a recent bottleneck event in this population, probably a consequence of the profound landscape transformation of the studied area during the last century; another possibility is this may be due to the observed deviation in the population sex ratio, which may be influencing the pumas' mating system. We propose that: (1) landscape management in the study area should be focused on increasing habitat connectivity, creating protected areas and structures to allow highway crossing of pumas;(2) educational actions should be undertaken to change community perception of large carnivores, and possibly the implementation of compensatory actions to ranchers.

show abstract

Development of 21 microsatellite loci for puma (Puma concolor) ecology and forensics

Cited by 27 publications

References 10 publications

Spatial and temporal interactions of sympatric mountain lions in Arizona

Spatial and temporal interactions of sympatric mountain lions in Arizona

Evidence of Recent Fine-Scale Population Structuring in South American Puma concolor

Genetic diversity and population structure of pumas (Puma concolor) in southeastern Brazil: implications for conservation in a human-dominated landscape

Contact Info

Product

Resources

About