Development of a Bacterial Surface Display of Hexahistidine Peptide Using CS3 Pili for Bioaccumulation of Heavy Metals

Abstract

A novel cell surface display system for metal uptake was developed using CS3 pili of enterotoxigenic Escherichia coli, which is a suitable system for display of heterologous peptides. The recombinant bacteria producing the hybrid pili containing the hexahistidine peptide accumulated high concentrations of Cd(2+) and Ni(2+) at 656.2 and 276.5 nmol per mg dry weight of bacterial cell, respectively. The recombinant bacteria may be useful in water and waste water treatment.

Immobilization of bacteriophage Qβ on metal-derivatized surfaces via polyvalent display of hexahistidine tags

“…The utility of polyhistidine and other metal-binding motifs incorporated into protein structures has expanded beyond protein purification to include applications in water purification [1], handles for protein-protein interactions to yield novel materials and structures [2], and surface immobilization. The latter use has received particular interest for applications in nanopatterning [3], biosensors [4] and surface-bound catalysts [5].…”

Selection and characterization of a 7‐mer peptide binding to divalent cations

“…In addition to metal detection, its use could be extended to biosorption of heavy metals from the environment. For example, it can be displayed on the surfaces of yeast17 or bacteria18, 19 and used to remove heavy metals.…”

Immobilization of recombinant nanobiofiber CS3 fimbriae onto alginate beads for improvement of cadmium biosorption

“…The cst operon (4.7 Kbp) encoding CS3 in ETEC has been cloned and sequenced (Gene bank accession No X16944). It consists of genes encoding pili subunits of 14.5 -17 kDa and pili biosynthesis proteins [17,28,29].…”

“…CS3 pili are thin, flexible, and wiry threads produced by certain Enterotoxigenic E. coli (ETEC) alone or together with other E. coli surface antigens. CS3 fimbriae have the advantage of tolerate insertions of heterologous peptides and are known as a suitable display system for the expression of foreign epitopes, including metal-binding peptides, on the surface of bacterial cells to develop a new metal biosorbent [16][17][18]. Although metal-binding peptides displayed on the bacterial surface have great potential to uptake heavy metals, their stabilization and recycling constitute technical obstacles to their industrial application.…”

“…In a previous study, a novel cell surface display system based on CS3 pili with surface-exposed domains was developed, and the efficiency of a cysteine-rich peptide displayed on the hybrid pili of free cells to accumulate bivalent metal ions was investigated [17,18]. There has been much research on the adsorption of metal ions by immobilized microbial cells [23][24][25].…”