Development of a bio-electrochemical assay for AFB1 detection in olive oil

Rejeb, Ines Ben; Arduini, Fabiana; Arvinte, Adina; Amine, Aziz; Gargouri, M.; Micheli, Laura; Bala, Camelia; Moscone, Danila; Palleschi, Giuseppe

doi:10.1016/j.bios.2008.10.002

Cited by 87 publications

(38 citation statements)

References 24 publications

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“…For AFB1-DNA adducts, AFB1-N 7 -Gua adduct, the most common adduct type, is primarily identiied and conirmed in vivo research studies [17][18][19][20][21][22], whereas the formamidopyridine AFB1 adduct (AFB1-FAPy) is a ring-opened DNA adduct [23,24]. Because of the accumulations of AFB1-FAPy, it is characterized by a time-dependent and non-enzyme way and exhibits apparent persistence in DNA; it may be of biological basis of genes mutation.…”

Section: Dna Damage Induced Byafb1 and Dna Repairmentioning

confidence: 99%

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Genetic Single Nucleotide Polymorphisms (GSNPs) in the DNA Repair Genes and Hepatocellular Carcinoma Related to Aflatoxin B1 among Guangxiese Population

Wu¹,

Xi²,

Lü³

et al. 2017

Genetic Polymorphisms

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Alatoxin B1 (AFB1) is an important environmental carcinogen for the development of hepatocellular carcinoma (HCC). HCC is a complex disease likely resulting from genetic single nucleotide polymorphisms (GSNPs) of multiple interacting genes and geneenvironment interactions. Recent eforts have been made to analyze the associations between risk of this malignancy and GSNPs in genes involved in the repair of DNA damage induced by AFB1. Here, we reviewed the results of published case-control studies that have examined the efects of common alleles of all susceptible DNA repair genes, including XRCC1, XRCC3, XRCC4, XRCC7, XPC, and XPD, on risk of AFB1-related HCC among Guangxi population. Statistically signiicant diferences in genotype frequencies found in case-control comparisons were rs25487, rs80309960, rs861539, rs7003908, rs28383151, rs3734091, rs13181, and rs2228001 polymorphism. The overall efects of these GNSPs were moderate in terms of relative risk, with ORs ranging from 2 to 10. Furthermore, some evidence of the interaction of GSNPs in DNA repair genes and AFB1 exposure modulate risk of this cancer was also found, although the results require conirmation with larger sample size studies.

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Section: Dna Damage Induced Byafb1 and Dna Repairmentioning

confidence: 99%

“…Once this toxigenic agent is taken into body, it is metabolized by phase I detoxiication enzymes to its reactive form, also called AFB1-8,9-epoxide (AFBO). This reactive product can also covalently bind to DNA and induce DNA damage, which might ultimately result to the development of HCC [16,17].…”

Section: Dna Damage Induced Byafb1 and Dna Repairmentioning

confidence: 99%

Genetic Single Nucleotide Polymorphisms (GSNPs) in the DNA Repair Genes and Hepatocellular Carcinoma Related to Aflatoxin B1 among Guangxiese Population

Wu¹,

Xi²,

Lü³

et al. 2017

Genetic Polymorphisms

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show abstract

“…Therefore, AFB1 has been classified as a category I known human carcinogen by the International Agency for Research on Cancer (24). AFB1 is metabolized by cytochrome P450 enzymes to its reactive form, AFB1-8,9-epoxide (AFB1-epoxide), which covalently binds to DNA and induces DNA damage (19,(25)(26)(27)(28). DNA damage induced by AFB1 includes AFB1-DNA adducts, oxidative DNA damage, www.intechopen.com and gene mutation (Fig.…”

Section: Afb1 Exposure and Dna Damage And Repairmentioning

confidence: 99%

DNA Repair Capacity-Related to Genetic Polymorphisms of DNA Repair Genes and Aflatoxin B1-Related Hepatocellular Carcinoma Among Chinese Population

Long¹,

Yao²,

Zeng³

et al. 2011

DNA Repair

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“…The mercapto groups are easily oxidized and the photocurrent response increases as a result of the effect of electron-hole pairs system. AFB 1 has a strong inhibitory effect on acetylcholinesterase and irreversibly noncompetitive inhibition by altering the site of action of acetylcholinesterase [18][19][20][21][22]. The reaction formula is as follows:…”

Section: Procedures For Electrochemical Biosensormentioning

confidence: 99%

Detection of AFB1 via TiO2 Nanotubes/Au Nanoparticles/Enzyme Photoelectrochemical Biosensor

Yuan

et al. 2018

Coatings

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TiO 2 nanotubes/Au nanoparticles/enzyme photoelectrochemical biosensor is developed by the chemical bonding of acetylcholinesterase with Au nanoparticles-modified TiO 2 photoactive electrode, based on the inhibitory effect of aflatoxin B 1 on acetylcholinesterase activity. In this method, AuNPs were deposited on the surface of the electrode by potentiostatic deposition and the acetylcholinesterase was chemically crosslinked to the surface for determination of aflatoxin B 1 . Enzymatic hydrolysate is generated to capture the photogenerated holes of UV-sensitized TiO 2 nanotube arrays, causing magnification of the photoelectrochemical signal. The photoelectrochemical biosensor morphological and structural details were evaluated, applying different techniques, such as X-ray Diffraction (XRD) and Scanning Electron Microscopy (SEM). Aflatoxin B 1 competitively inhibits acetylcholinesterase, leading to a decrease in photocurrent that should have been increased. The detection performance of biosensors for different concentrations of AFB 1 is discussed. The linear response range of the biosensor is from 1-6 nM with detection limitation of 0.33 nM, the linear equation is I (µA) = −0.13C (nM) + 9.98 (µA), with a correlation coefficient of 0.988. This new biosensor could be used to detect Aflatoxin B 1 in foods.

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Development of a bio-electrochemical assay for AFB1 detection in olive oil

Cited by 87 publications

References 24 publications

Genetic Single Nucleotide Polymorphisms (GSNPs) in the DNA Repair Genes and Hepatocellular Carcinoma Related to Aflatoxin B1 among Guangxiese Population

Genetic Single Nucleotide Polymorphisms (GSNPs) in the DNA Repair Genes and Hepatocellular Carcinoma Related to Aflatoxin B1 among Guangxiese Population

DNA Repair Capacity-Related to Genetic Polymorphisms of DNA Repair Genes and Aflatoxin B1-Related Hepatocellular Carcinoma Among Chinese Population

Detection of AFB1 via TiO2 Nanotubes/Au Nanoparticles/Enzyme Photoelectrochemical Biosensor

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