Development of a Biosensor Based on Phosphotriesterase and n‐Channel ISFET for Detection of Pesticides

Andrianova, Mariia; Gubanova, O. V.; Комарова, Н. В.; Кузнецов, Е. В.; Kuznetsov, Alexander

doi:10.1002/elan.201500411

Cited by 26 publications

(18 citation statements)

References 44 publications

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“…Moreover, the techniques require trained technicians to perform the analysis competently [41]. Thus, portable and specific enzymatic biosensors (especially for use in field OP monitoring), with high selectivity and sensitivity, would be very beneficial [42,43].…”

Section: Biosensorsmentioning

confidence: 99%

“…In enzymatic biosensing of OP compounds, the detection is based on either their inhibition of AChE or the hydrolysis process by the OP hydrolysis enzyme [42,43]. Detection using an inhibition-based sensor has been reported to have a high sensitivity and is capable of ultralow OP detection [42,44].…”

Section: Biosensorsmentioning

confidence: 99%

“…Therefore, direct measurement of the hydrolysis reaction is preferable compared to just measurement of inhibition. Furthermore, the detection limit of sensors based on the PTE reaction is better when compared with that of AChE activity detection [31,43].…”

Section: Biosensorsmentioning

confidence: 99%

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Microbial Phosphotriesterase: Structure, Function, and Biotechnological Applications

et al. 2019

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The role of phosphotriesterase as an enzyme which is able to hydrolyze organophosphate compounds cannot be disputed. Contamination by organophosphate (OP) compounds in the environment is alarming, and even more worrying is the toxicity of this compound, which affects the nervous system. Thus, it is important to find a safer way to detoxify, detect and recuperate from the toxicity effects of this compound. Phosphotriesterases (PTEs) are mostly isolated from soil bacteria and are classified as metalloenzymes or metal-dependent enzymes that contain bimetals at the active site. There are three separate pockets to accommodate the substrate into the active site of each PTE. This enzyme generally shows a high catalytic activity towards phosphotriesters. These microbial enzymes are robust and easy to manipulate. Currently, PTEs are widely studied for the detection, detoxification, and enzyme therapies for OP compound poisoning incidents. The discovery and understanding of PTEs would pave ways for greener approaches in biotechnological applications and to solve environmental issues relating to OP contamination.

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Section: Biosensorsmentioning

confidence: 99%

Section: Biosensorsmentioning

confidence: 99%

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Microbial Phosphotriesterase: Structure, Function, and Biotechnological Applications

et al. 2019

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“…Kinetic measurements were performed at room temperature for 4 h, and the increase in absorbance at 650 nm was recorded. The measurement of nitroreductase activity using an ISFET was performed according to a method previously described [18], using a semiconductor device parameter analyzer (Agilent B1500A, Santa Clara, CA, USA) and a probe station (CascadeMicrotech PM5, Beaverton, OR, USA) operated by the Agilent VEE Pro software (Version A.04.20).…”

Section: Synthesis and Characterization Of Bocapsmentioning

confidence: 99%

A Surface Modifier for the Production of Selectively Activated Amino Surface Groups

et al. 2019

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The formation of self-assembled monolayers with the possibility of selective activation is an important goal of surface chemistry. In this work, a new surface modifier which creates amino surfaces based on aminopropylsilatrane (APS) with a protected amino group was obtained. The utilization of protected APS allows producing a self-assembled monolayer (SAM) and obtaining reactive surface amino groups at distinct times. Furthermore, a precise selective deprotection with a further modification of the activated amino groups could be performed without affecting the protected groups. To demonstrate the practical applicability of this modifier, a trinitrotoluene-sensitive sensor based on an ion-sensitive field-effect transistor (ISFET) was obtained.

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“…Andrianova et al used a microfluidic device to deliver an analyte to a CMOS‐compatible n‐channel ISFET, which was able to detect the presence of organophosphorus pesticides. The pesticides were immobilized on the surface of ISFETs and a phosphodiesterase enzyme was used to enhance sensitivity . Demircan et al designed a label‐free LOC detection system that integrated DEP and CMOS imaging for leukemia cell detection .…”

Section: Applications Of Cmos Based Microfluidic Devicesmentioning

confidence: 99%

CMOS Enabled Microfluidic Systems for Healthcare Based Applications

et al. 2018

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additional parts, such as syringe pumps, control valves, and microscopes, which not only increase the complexity and cost of the system but also create a dependence of the system on a laboratorybased environment. Technology has now advanced so much that there are fully integrated sample-in-results-out chips for genetic analysis, [8] sensors that can detect a handful of molecules, and many other similar systems. [9] Complementary metal oxide semiconductor (CMOS) integrated LOC systems can be developed using CMOS technologies as sensors, signal conditioners, data processing circuitry, actuators, and wireless communication capabilities. [10][11][12][13][14][15][16][17] CMOS is a set of microfabrication and nanofabrication processes, which is used to manufacture semiconductor chips present inside computer, smartphone, or any electronic gadgets. Consequently, these chips are referred to as CMOS enabled or CMOS based chips. CMOS based chips can act as a processor, memory, and digital logic circuitry for data management. In this review, we will discuss how CMOS based devices have enabled both management of data from microfluidic devices as well as sensors and actuators to help complement the functionality of LOC devices. CMOS logics are simple to make, and consume little to no current in idle state. With the advancements in Moore's law we have seen the size of CMOS devices getting smaller and smaller allowing for more functionality to fit in a smaller area. [18] CMOS plays a significant role in the realization of portable and versatile microfluidic platforms by integrating them with microscale sensors, interfaces, and actuators to form autonomous hybrid systems. [10,12,16] Adding interface electronics to the same CMOS chip as sensors, actuators, data processing, and communication capabilities creates a universal platform for LOC technologies. Having signal amplification and noise removal right at the sensor interface without any signal loss makes the device output more accurate and sensitive, and also removes parasitic and mismatch errors. [1] By using CMOScompatible processes, it is possible to interface directly with the readout circuitry to form an integrated circuit system on a single chip. Signal acquisition from a large array of sensors to a single system requires a complex integration scheme, but CMOS microfabrication processes enable the integration of analogue multiplexers with biosensors on small dice. [19] CMOS also has the capability to actuate pumps and valves in microfluidic devices or to directly influence the fluid itself using electric With the increased global population, it is more important than ever to expand accessibility to affordable personalized healthcare. In this context, a seamless integration of microfluidic technology for bioanalysis and drug delivery and complementary metal oxide semiconductor (CMOS) technology enabled data-management circuitry is critical. Therefore, here, the fundamentals, integration aspects, and applications of CMOS-enabled microfluidic systems for affordable persona...

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Development of a Biosensor Based on Phosphotriesterase and n‐Channel ISFET for Detection of Pesticides

Cited by 26 publications

References 44 publications

Microbial Phosphotriesterase: Structure, Function, and Biotechnological Applications

Microbial Phosphotriesterase: Structure, Function, and Biotechnological Applications

A Surface Modifier for the Production of Selectively Activated Amino Surface Groups

CMOS Enabled Microfluidic Systems for Healthcare Based Applications

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