Development of a cDNA‐AFLP derived CAPS marker co‐segregating with the powdery mildew resistance gene Mlg in barley

Abstract

Powdery mildew is an important pathogen of barley in many barleygrowing areas. To identify molecular markers for the resistance gene Mlg located on chromosome 4H, a cDNA-AFLP approach was carried out on near-isogenic lines. Based on the identification of a differentiating fragment of 37 bp, which turned out to be part of a nucleoside diphosphate kinase as estimated by BLASTN, a CAPS marker has been developed, which co-segregated with Mlg in a population of 738 F 2 -plants. Due to its co-dominant inheritance, clear banding pattern and close linkage this marker is well suited for marker-based selection procedures.

Analysis of powdery mildew resistance in the Spanish barley core collection

“…The absence of linkage between MlLa (2H) and Mlg (4H) (Chelkowski et al. 2003, Korell et al. 2008) facilitated their use in combination.…”

“…The genes MlLa (resistance ÔLaeviga-tumÕ) and Mlg (resistance ÔWeihenstephanÕ) have been the most widely used in European barley breeding (Brown and Jørgen-sen 1991). The absence of linkage between MlLa (2H) and Mlg (4H) (Chelkowski et al 2003, Korell et al 2008) facilitated their use in combination. These two genes together with Mlh (from ÔHautersÕ) were among the first genes widely introduced in Europe, especially in winter barley (Brown and Jørgensen 1991).…”

Application of whole genome re-sequencing data in the development of diagnostic DNA markers tightly linked to a disease-resistance locus for marker-assisted selection in lupin (Lupinus angustifolius)

“…The most desirable markers for MAS are “co-segregating”, where marker genotypes are completely consistent with plant phenotypes in segregating breeding populations. Co-segregating markers offer maximum accuracy on MAS [ 6 , 7 ]. Secondly, the genotypes of the markers should match plant phenotypes in a wide range of breeding germplasm, allowing broad application in a breeding program.…”