Development of a co-dominant SCAR marker linked to the Ph-3 gene for Phytophthora infestans resistance in tomato (Solanum lycopersicum)

Truong, Hai Thi Hong; Tran, Hung Ngoc; Choi, Hak Soon; Park, Pue Hee; Lee, Hye-Eun

doi:10.1007/s10658-012-0157-4

Cited by 13 publications

(17 citation statements)

References 25 publications

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“…Three published Ph‐3 markers, SCU602F3R3 (SCAR; Truong et al. ), SCAR‐Ph3‐3 (SCAR; Park et al. ) and TG328/ Bst NI (CAPS; Robbins et al.…”

Section: Resultsmentioning

confidence: 99%

“…Marker SCU602F3R3 accurately measured the genotypes of the control plants (expect for LBR‐11; Table ), and the PCR patterns resulting from resistant and susceptible individuals were as described in Truong et al. () (data not shown). However, according to SCU602F3R3, RIL genotypes did not match the phenotypes.…”

Section: Resultsmentioning

confidence: 99%

“…In this study, Ph3.gsm/ Hin cII gene‐specific marker was developed and its effectiveness was compared with other Ph‐3 markers including SCU602F3R3 (Truong et al. ), SCAR‐Ph3‐3 (Park et al. ) and TG328/ Bst NI (Robbins et al.…”

Section: Discussionmentioning

confidence: 99%

“…), SCU602F3R3 (SCAR; Truong et al. ), SCAR‐Ph3‐3 (SCAR; Park et al. ) and Ph3.gsm/ Hinc II, were used to infer the presence of the Ph‐3 allele.…”

Section: Methodsmentioning

confidence: 99%

“…, Truong et al. ). The recent cloning and characterization of Ph‐3 gene in L3708 revealed that it encodes a coiled‐coil nucleotide‐binding leucine‐rich repeat (CC‐NBS‐LRR) protein and can provide a susceptible tomato cultivar with late blight resistance via gene transformation (Zhang et al.…”

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confidence: 99%

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Evaluation of the Ph‐3 gene‐specific marker developed for marker‐assisted selection of late blight‐resistant tomato

et al. 2016

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Late blight caused by Phytophthora infestans is one of the most destructive diseases of tomato (Solanum lycopersicum L.) that mainly occurs in cool and wet environments. With the spread of the A2 mating type and new clonal lineages, fewer fungicides provide effective control of the disease, which has increased its worldwide threat. Host resistance could contribute significantly to sustainable disease control. Ph-3 is a race-specific late blight resistance gene commonly used in commercial tomato breeding. Availability of precise and easy to use gene-based markers would facilitate selection. In this study, a Ph-3 on-gene cleaved amplified polymorphic sequence (CAPS) marker, Ph3.gsm/HincII, was developed based on the published gene sequence of Ph-3. The effectiveness of the marker was evaluated along with other published Ph-3 markers using an F 9 recombinant inbred line (RIL) population derived from NC 23E-2 (93) 9 L3708. Markers Ph3.gsm/HincII and TG328/BstNI accurately genotyped the RIL population for Ph-3. In addition, Ph3.gsm/HincII was able to differentiate variable susceptible alleles. This reliable codominant DNA marker would be very useful in marker-assisted selection, particularly for resistance gene pyramiding.

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“…Three published Ph‐3 markers, SCU602F3R3 (SCAR; Truong et al. ), SCAR‐Ph3‐3 (SCAR; Park et al. ) and TG328/ Bst NI (CAPS; Robbins et al.…”

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“…), SCU602F3R3 (SCAR; Truong et al. ), SCAR‐Ph3‐3 (SCAR; Park et al. ) and Ph3.gsm/ Hinc II, were used to infer the presence of the Ph‐3 allele.…”

Section: Methodsmentioning

confidence: 99%

mentioning