Development of a collagen-like peptide polymer via end-to-end disulfide cross-linking and its application as a biomaterial

Ichise, shin-ichiro; Takeuchi, Shungo; Aoki, Shigehisa; Kuroda, Kazuki; Nosé, Hiroshi; Masuda, Ryo; Koide, Takao

doi:10.1016/j.actbio.2019.06.010

Cited by 12 publications

(16 citation statements)

References 47 publications

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“…In contrast to the native type I collagen gel, no fibrillar structures or pore structures were observed in the polymer gel. The structure was very similar to the gel developed in our previous study [23].…”

Section: Investigation Of the Gel-forming Conditions Of The Copolymer...supporting

confidence: 79%

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Synthetic Collagen-like Polymer That Undergoes a Sol–Gel Transition Triggered by O–N Acyl Migration at Physiological pH

Ichise

Koide

2022

IJMS

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We previously reported an artificial collagen gel that can be used as a cell-culture substrate by end-to-end cross-linking of collagen-like triple-helical peptides via disulfide bonds. However, the gel had to be formed a priori by polymerizing the peptide in an acidic solution containing dimethyl sulfoxide for several days, which prevented its use as an injectable gel or three-dimensional (3D) scaffold for cell culture. In this study, we developed a collagen-like peptide polymer by incorporating an O–N acyl migration-triggered triple helix formation mechanism into a collagen-like peptide, which formed a gel within 10 min. We demonstrated that the collagen-like peptide polymer can be used as a 3D cell scaffold and that the 3D structure formation of cells can be controlled by collagen-derived bioactive sequences introduced into the peptide sequence.

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Section: Investigation Of the Gel-forming Conditions Of The Copolymer...supporting

confidence: 79%

“…Recently, we developed another artificial collagen system by end-to-end cross-linking of collagen-like triple-helical peptides [23]. We found that a peptide with two or more Cys residues at each terminus of the collagen-like sequence formed a gel by triple helix formation and subsequent cross-linking via disulfide bonds.…”

Section: Introductionmentioning

confidence: 99%

Synthetic Collagen-like Polymer That Undergoes a Sol–Gel Transition Triggered by O–N Acyl Migration at Physiological pH

Ichise

Koide

2022

IJMS

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“…With the incorporation of an integrin α 2 β 1 -binding sequence, the peptide polymer showed receptor-specific cell binding in vitro. Moreover, cell signaling activity and biodegradability of such a system can be tuned by altering the content of integrin binding ligand in the peptide polymer and by varying the weight percentage of CLP [ 242 ].…”

Section: Applications In Drug Deliverymentioning

confidence: 99%

Recent trends in protein and peptide-based biomaterials for advanced drug delivery

Varanko

Saha

Chilkoti

2020

Advanced Drug Delivery Reviews

233

120

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Engineering protein and peptide-based materials for drug delivery applications has gained momentum due to their biochemical and biophysical properties over synthetic materials, including biocompatibility, ease of synthesis and purification, tunability, scalability, and lack of toxicity. These biomolecules have been used to develop a host of drug delivery platforms, such as peptide- and protein-drug conjugates, injectable particles, and drug depots to deliver small molecule drugs, therapeutic proteins, and nucleic acids. In this review, we discuss progress in engineering the architecture and biological functions of peptide-based biomaterials —naturally derived, chemically synthesized and recombinant— with a focus on the molecular features that modulate their structure-function relationships for drug delivery.

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“…Specifically, polymerization consisted in the formation of disulfide bonds among cysteine residues (Cys) present at both ends of the peptides; the number of Cys residues allowed to modulate the Young’s modulus and produce gels with specific mechanical properties (40.6 ± 6.2 kPa, 671 ± 67 kPa, and 709 ± 63 kPa). Finally, the incorporation of the binding sequence for the integrin α2β1 promoted cell adhesion . A similar strategy is represented by the use of modified amphiphilic peptides to allow self-assembly through host–guest interactions .…”

Section: D Matrix With Tunable Stiffnessmentioning

confidence: 99%

3D Cell Culture: Recent Development in Materials with Tunable Stiffness

Baruffaldi

Palmara

Pirri

et al. 2021

ACS Appl. Bio Mater.

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It is widely accepted that three-dimensional cell culture systems simulate physiological conditions better than traditional 2D systems. Although extracellular matrix components strongly modulate cell behavior, several studies underlined the importance of mechanosensing in the control of different cell functions such as growth, proliferation, differentiation, and migration. Human tissues are characterized by different degrees of stiffness, and various pathologies (e.g., tumor or fibrosis) cause changes in the mechanical properties through the alteration of the extracellular matrix structure. Additionally, these modifications have an impact on disease progression and on therapy response. Hence, the development of platforms whose stiffness could be modulated may improve our knowledge of cell behavior under different mechanical stress stimuli. In this review, we have analyzed the mechanical diversity of healthy and diseased tissues, and we have summarized recently developed materials with a wide range of stiffness.

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Development of a collagen-like peptide polymer via end-to-end disulfide cross-linking and its application as a biomaterial

Cited by 12 publications

References 47 publications

Synthetic Collagen-like Polymer That Undergoes a Sol–Gel Transition Triggered by O–N Acyl Migration at Physiological pH

Synthetic Collagen-like Polymer That Undergoes a Sol–Gel Transition Triggered by O–N Acyl Migration at Physiological pH

Recent trends in protein and peptide-based biomaterials for advanced drug delivery

3D Cell Culture: Recent Development in Materials with Tunable Stiffness

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