Development of a colloidal gold strip for rapid detection of ochratoxin A with mimotope peptide

Increased sensitivity of lateral flow immunoassay for ochratoxin A through silver enhancement

“…Effective advantages in terms of gain on sensitivity were demonstrated on a competitive LFIA for measuring ochratoxin A in wine and grape must, previously developed on a gold-based model. The IC 50 for the "ultrasensitive" silver enhanced-LFIA was more than 10-folds lower compared to the one of the gold-based LFIA, and, based on our knowledge, the developed method is the most sensitive LFIA for measuring ochratoxin A [23][24][25][26][27]. This assay exceeded requirements for measuring OTA in beverages according to the legislation in force.…”

“…However, immunochemical analyses are largely employed as screening methods, thanks to their simplicity, rapidity and cost effectiveness [16,[20][21][22]. LFIAs aimed at measuring OTA in food, beverages and feed have been reported, as well [23][24][25][26][27].…”

Phage-displayed peptides that mimic zearalenone and its application in immunoassay

“…There are many advantages of phage ELISA contrast to conventional ciELISA for ZEN: (a) phage ELISA for ZEN detect a lower range of concentrations (100-10,000 pg/ml) than conventional ciELISA; (b) the preparation of phage peptides is easier and cheaper than ZEN standard solution and the phage peptides are non-toxic and result in enhanced laboratory and environmental safety; and (c) in addition to as competitive antigen in icELISA, phage peptides could be used as substitute detection antigen coated in microplate for ELISA detection, avoiding the synthesis of antigen by chemical methods (Lai, Fung, Xu, Liu, & Xiong, 2009). …”

Aflatoxin Measurement and Analysis

“…requiring only a sample extraction step before use; 2. simplicity of procedure with single step, e.g., only adding test solution to the sample pad on the strip; 3. rapid on-site detection within a few minutes (5-15 min); 4. concentration levels of target analytes can be observed directly with the naked eyes; 5. user-friendly format no need for skill personnel; 6. less interference due to chromatographic separation; and 7. low cost Because of these advantages, lateral flow strip assay has become one of the commercial and widely-used immunoassays for rapid determination of mycotoxins, such as ochratoxin A (Lai et al, 2009;Liu, Tsao, Wang, & Yu, 2008;Wang, Liu, Xu, Zhang, & Wang, 2007;Cho et al, 2005), deoxynivalenol (Kolosova, De Saeger, Sibanda, Verheijen, & Van Peteghem, 2007;Xu et al, 2010;Kolosova et al, 2008), T-2 Toxin (Molinelli et al, 2008), zearalenone (Kolosova, De Saeger, Sibanda, Verheijen, & Van Peteghem, 2007), fumonisin B1 (Wang, Quan, Lee, & Kennedy, 2006), aflatoxins (Sun, Zhao, Tang, Zhou, & Chu, 2005;Sheibani, Tabrizchi, & Ghaziaskar, 2008) and so on. The visual detection limit (VDL), defined as the minimum concentration producing the color on the test line significantly different or weaker to that on the test line of negative control strip without aflatoxin (Li, Wei, Yang, Li, & Deng, 2009;Zhou et al, 2009), was used to express the sensitivity of the lateral flow strip assay.…”