Development of a colloidal gold immunochromatographic strip for rapid detection of Riemerella anatipestifer in ducks

Abstract

Riemerella anatipestifer is one of the major bacterial pathogens of ducks and causes significant economic losses in poultry agriculture. Usually, methods for detecting R. anatipestifer infection need specialized equipment and highly skilled personnel. In this study, a novel colloidal gold immunochromatographic strip was developed for rapid detection of R. anatipestifer in ducks. The monoclonal antibodies 2D5 and 2A6 against R. anatipestifer were used as colloidal gold-labeled protein and capture protein, respectively, to recognize the bacteria in tryptic soy broth medium culture and in hearts of infected ducks. The goat anti-mouse IgG antibody was labeled on nitrocellulose membrane as a control for C line. The labeling pH was optimized as 10.0, and the concentration of 2D5 labeled to colloidal gold particles was optimized as 18 μg/mL. The strip specifically detected serotypes 1, 2, and 10 R. anatipestifer strains and showed no cross-reaction with Escherichia coli , Salmonella enterica, and Pasteurella multocida strains. The sensitivity of the strip for detecting R. anatipestifer was 1.0 × 10 6 colony forming unit. The strips remained stable for up to 8 mo at 4°C, and the detection can be completed within 15 min. The strip can detect R. anatipestifer in hearts of the ducks experimentally infected with R. anatipestifer but not infected with E. coli , which were also confirmed with bacterial isolation followed by multiplex polymerase chain reaction. These results suggested that the strips are reliable methods for identification of R. anatipestifer in laboratories and in duck farms.

Epidemiological investigation and drug resistance characteristics of Riemerella anatipestifer strains from large-scale duck farms in Shandong Province, China from March 2020 to March 2022

“…The isolation rate was about 16.9% of R. anatipestifer and serotype 10 was the majority in Vietnam ( Vo et al, 2022 ). Sixteen serotypes have been isolated and identified in China ( Zhai et al, 2013 ) with serotypes 1, 2, and 10 being the most prevalent ( Han et al, 2020 ). In this study, the serotypes of duck disease in Shandong Province were mainly serotypes 1, 2, 6, and 7.…”

Development of Immunochromatographic Assay for the Rapid Detection of Mycoplasma capricolum subsp. capripneumoniae Antibodies

“…The minimum concentration of antibody that made the red color of the mixture unchanged was regarded as the optimum concentration of rabbit anti-goat IgG for colloidal gold labeling. The rabbit anti-goat IgG-coated colloidal gold probe was prepared as previously described ( Han et al, 2020 ) and stored at 4°C in the dark for further use.…”

Immunological quantitative detection of dicofol in medicinal materials

“…In this study, colloidal gold particles were synthesized using the trisodium citrate reduction method as previously reported. 23,24 The specific synthesis steps for colloidal gold particles are as follows. First, 100 mL of HAuCl 4 (0.01%, w/v) solution was heated to boiling with continuous stirring; then, 4 mL of trisodium citrate (1%, w/v) solution was added and heating was continued for another 10 min.…”