Development of a DNA chip to identify the place of origin of hairtail species

Park, Jung Youn; Kim, Jihoon; Kim, Eun‐Mi; Kang, Jung-Ha; Kang, Hyun-Suk; An, Cheul Min; Lee, Won Sun; Hwang, Seung Yong

doi:10.1007/s13206-013-7206-8

Cited by 8 publications

(2 citation statements)

References 23 publications

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“…5 Some of these include sequencing of short standardized gene regions, i.e., DNA barcoding, 6 of the mitochondrial gene cytochrome c oxidase 1 (CO1), 7,8 polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP), 9 and DNA microarrays with fluorescence detection. 10–12 Kochzius et al 11 found that the mitochondrial 16S gene yielded more effective probes than CO1 in a fluorescently detected microarray. However, CO1 was successfully used by Park et al 12 in a microarray format to identify hairtail fish species.…”

Section: Introductionmentioning

confidence: 99%

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Microarray Chip Development Using Infrared Imaging for the Identification of Catfish Species

et al. 2014

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Several families of catfish species are extensively aquacultured around the world; however, only those from the family Ictaluridae can be labeled as catfish in the United States. Non-Ictalurid catfish species that are marketed as "catfish" in the USA are considered misbranded. Misbranding in general has led to an increased interest in developing deoxyribonucleic acid (DNA)-based methods such as DNA barcoding, polymerase chain reaction restriction fragment length polymorphism, and DNA microarrays with fluorescence detection for the identification of fish species. In this proof-of-concept study, DNA microarrays coupled with a newly developed mid-infrared imaging detection method were applied to the identification of seven species of catfish for the first time. Species-specific DNA probes targeting three regions per species of the cytochrome c oxidase 1 (barcoding) gene were developed and printed as microarrays on glass slides. Deoxyribonucleic acid targets labeled with biotin were hybridized to their complementary probes using a strategy that allowed the selective formation of a silver layer on hybridized spots needed for detection. Using this three-probe format, the seven species were all identified correctly, even when a limited number of false positive spots were observed. Raman spectroscopy was employed to further characterize the arrays.

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Section: Introductionmentioning

confidence: 99%

“…10–12 Kochzius et al 11 found that the mitochondrial 16S gene yielded more effective probes than CO1 in a fluorescently detected microarray. However, CO1 was successfully used by Park et al 12 in a microarray format to identify hairtail fish species.…”

Section: Introductionmentioning

confidence: 99%