Methods for Seafood Authenticity Testing in Europe

Verrez‐Bagnis, Véronique; Sotelo, Carmen G.; Mendes, Rogério; Silva, Helena; Kappel, K. van; Schröder, Ute

doi:10.1007/978-3-319-78030-6_69

Cited by 10 publications

(12 citation statements)

References 320 publications

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“…Is widely recognize that in highly processed food (canned or cooked), the DNA is fragmented below 300 bp, leading to extracting less and degraded DNA [9,67]. The HRM analysis use amplicons shorter than 300 bp [11] and the sensitivity of detection is enhanced in smaller amplicons [65]; therefore, it is ideal for genetic analysis of highly processed food. In our method, the size of the amplicons sizes were between 50 to 170 bp, appropriated to be successfully genotyped in processed food.…”

Section: Fitness For Purpose Assessment Of the Multi-locus Pcr-hrm Methodsmentioning

confidence: 99%

“…It can also affect food safety via unnoticed consumption of allergens due to undeclared species [7,8]. Seafood mislabeling is well documented throughout history [9,10]; it impacts not only food authenticity [11] but also allows the trade in the markets of endangered species or products from illegal, unreported and unregulated (IUU) fisheries, threatening wildlife [12], hampering conservation and negatively affecting consumers decisions [13]. Nowadays, the breadth and depth of mislabeling are coming into sharper focus, thanks to DNA-based species verification methods [14].…”

Section: Introductionmentioning

confidence: 99%

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Development and Validation of a Multi-Locus PCR-HRM Method for Species Identification in Mytilus Genus with Food Authenticity Purposes

Quintrel

Jilberto

Sepúlveda

et al. 2021

Foods

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DNA-based methods using informative markers such as single nucleotide polymorphism (SNPs) are suitable for reliable species identification (SI) needed to enforce compliance with seafood labelling regulations (EU No.1379/2013). We developed a panel of 10 highly informative SNPs to be genotyped by PCR-High resolution melting (HRM) for SI in the Mytilus genus through in silico and in vitro stages. Its fitness for purpose and concordance were assessed by an internal validation process and by the transference to a second laboratory. The method was applicable to identify M. chilensis, M. edulis, M. galloprovincialis and M. trossulus mussels, fresh, frozen and canned with brine, oil and scallop sauce, but not in preserves containing acetic acid (wine vinegar) and tomato sauce. False-positive and negative rates were zero. Sensitivity, expressed as limit of detection (LOD), ranged between 5 and 8 ng/μL. The method was robust against small variations in DNA quality, annealing time and temperature, primer concentration, reaction volume and HRM kit. Reference materials and 220 samples were tested in an inter-laboratory assay obtaining an “almost perfect agreement” (κ = 0.925, p < 0.001). In conclusion, the method was suitable for the intended use and to be applied in the seafood industry.

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Section: Fitness For Purpose Assessment Of the Multi-locus Pcr-hrm Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Development and Validation of a Multi-Locus PCR-HRM Method for Species Identification in Mytilus Genus with Food Authenticity Purposes

Quintrel

Jilberto

Sepúlveda

et al. 2021

Foods

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“…However, they noted that wild-caught or mariculture production was underestimated, and further work would be required to "ground-truth" or verify the provenance of seafood. Furthermore, the existing databases in countries like Europe focus on utilising DNA profiles of seafood [19,20]. Another database of note is the Barcode of Life Data System, which contains the DNA barcodes of 244k animal species [21] and has been successfully used to detect species mislabelling [22].…”

Section: Introductionmentioning

confidence: 99%

Developing a MySQL Database for the Provenance of Black Tiger Prawns (Penaeus monodon)

Gopi

Mazumder

Crawford

et al. 2023

Foods

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As the demand for seafood increases, so does the incidence of seafood fraud. Confirming provenance of seafood is important to combat fraudulent labelling but requires a database that contains the isotopic and elemental “fingerprints” of authentic seafood samples. Local isotopic and elemental databases can be scaled up or combined with other databases to increase the spatial and species coverage to create a larger database. This study showcases the use of isotopic and elemental fingerprints of the black tiger prawn (Penaeus monodon) to develop a database that can be used to securely store the data necessary for determining provenance. The utility of this database was tested through querying and building seven different datasets that were used to develop models to determine the provenance of P. monodon. The models built using the data retrieved from the database demonstrated that the provenance of P. monodon could be determined with >80% accuracy. As the database was developed using MySQL, it can be scaled up to include additional regions, species, or methodologies depending on the needs of the users. Combining the database with methods of determining provenance will provide regulatory bodies and the seafood industry with another provenance tool to combat fraudulent seafood labelling.

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“…in the markets, as well as served cooked in restaurants. Under these conditions, their morphological characteristics are altered; thus, identification of the species is often not possible, raising opportunities for fraudulent operations [ 8 , 9 ].…”

Section: Introductionmentioning

confidence: 99%

High-Resolution Melting (HRM) Analysis for Rapid Molecular Identification of Sparidae Species in the Greek Fish Market

Chatzoglou,

Tsaousi,

Apostolidis

et al. 2023

Genes

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The red porgy (Pagrus pagrus) and the common dentex (Dentex dentex) are Sparidae species of high commercial value, traded in the Greek market. In some cases, fish species identification from Greek fisheries is difficult for the consumer due to the strong morphological similarities with their imported counterparts or closely related species such as Pagrus major, Pagrus caeroleustictus, Dentex gibbosus and Pagellus erythrinus, especially when specimens are frozen, filleted or cooked. Techniques based on DNA sequencing, such as COI barcoding, accurately identify species substitution incidents; however, they are time consuming and expensive. In this study, regions of mtDNA were analyzed with RFLPs, multiplex PCR and HRM in order to develop a rapid method for species identification within the Sparidae family. HRM analysis of a 113 bp region of cytb and/or a 156 bp region of 16s could discriminate raw or cooked samples of P. pagrus and D. dentex from the aforementioned closely related species and P. pagrus specimens sampled in the Mediterranean Sea when compared to those fished in the eastern Atlantic. HRM analysis exhibited high accuracy and repeatability, revealing incidents of mislabeling. Multiple samples can be analyzed within three hours, rendering this method a useful tool in fish fraud monitoring.

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Methods for Seafood Authenticity Testing in Europe

Cited by 10 publications

References 320 publications

Development and Validation of a Multi-Locus PCR-HRM Method for Species Identification in Mytilus Genus with Food Authenticity Purposes

Development and Validation of a Multi-Locus PCR-HRM Method for Species Identification in Mytilus Genus with Food Authenticity Purposes

Developing a MySQL Database for the Provenance of Black Tiger Prawns (Penaeus monodon)

High-Resolution Melting (HRM) Analysis for Rapid Molecular Identification of Sparidae Species in the Greek Fish Market

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