Development of a fixed-bed anammox reactor with high treatment potential

Okamoto, Hiroyuki; Kawamura, Kiyoko; Nishiyama, Takashi; Fujii, Tomoyuki; Furukawa, Koichi

doi:10.1007/s10532-012-9561-x

Cited by 22 publications

(11 citation statements)

References 14 publications

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“…Industrial application of the ANAMMOX process is usually hindered by the slow growth rate of ANAMMOX bacteria. Granulation and immobilization technology are the most acceptable ways to promote the application . Extracellular polymeric substances (EPS) excreted by microorganisms are a core factor in self‐granulation, which enhances physicochemical properties and biofacies structure .…”

Section: Introductionmentioning

confidence: 99%

ANAMMOX performance, granulation, and microbial response under COD disturbance

Huang

Ruan

et al. 2014

J. Chem. Technol. Biotechnol.

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BACKGROUND: ANAMMOX (ANaerobic AMMonium OXidation), a promising process for biological nitrogen removal, is usually affected by organic matter. In order to fully understand the effect of chemical oxygen demand (COD) on the ANAMMOX process, reactor performance, granular characteristics and microbial response were evaluated systematically under glucose stimulation. RESULTS: High removal efficiencies of ammonium and total nitrogen (> 90%) were achieved with 100 mg L -1 COD concentration. ANAMMOX contribution was reduced to only 69% as COD concentration was increased to 300 mg L -1 . The granulation, EPS contents, TB-EPS/LB-EPS and protein/carbohydrate were increased at a specific COD, with higher COD disrupting the biofacies structure. The results also showed that EPS distribution and microbial structure were affected by the organic disturbance. CONCLUSION: An appropriate COD concentration enhanced the total nitrogen removal during ANAMMOX process, by enabling a stable synergism between ANAMMOX bacteria and heterotrophic denitrificans. Furthermore, a proper COD stimulation promoted sludge granulation by influencing EPS composition and distribution. However, excessive COD disturbance disrupted the highly efficient biofacies structure and microbial balance, which then decreased the ANAMMOX competitiveness.

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Section: Introductionmentioning

confidence: 99%

ANAMMOX performance, granulation, and microbial response under COD disturbance

Huang

Ruan

et al. 2014

J. Chem. Technol. Biotechnol.

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“…In this study, we developed a new perfused microfluidic system that can provide several advantages over the commonly used cell coculture systems [Okamoto et., 2013;Chapman et al, 2014;Wan et al, 2016]. It was designed to have tandem culture chambers allowing cells to acquire nutrients and the secreted growth factors from adjacent cells.…”

Section: Discussionmentioning

confidence: 99%

“…A perfused culture can constantly provide continuous nutrient supply as well as removing the waste, thus maintaining a fresh and stable local microenvironment. Some perfused culture systems have been developed for use for either cell expansion [Okamoto et al, 2013;Chapman, 2014] or three-dimensional cell culture [Parrish et al, 2018;Vormann et al, 2018].…”

Section: Introductionmentioning

confidence: 99%

A Perfused Microfluidic System to Study the Differentiation of Neural Stem Cells in vitro

Liu

Xing

Liu

et al. 2018

Cells Tissues Organs

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Introduction: Due to the ability to mimic in vivo cellular microenvironments, the development of multicell culture systems has received increasing interest for use as research models and serving as platforms for drug evaluation. Methods: In this study, we developed a perfused microfluidic system to resemble the in vivo intercellular environment and applied it to study the differentiation from neural stem cells into neurons. Results: As determined by immunofluorescence chemistry and quantitative real-time PCR, the neural stem cells grown in this microfluidic system showed an elevated differentiation rate toward the formation of neurons as determined by the increased level of βIII-tubulin production, which is 4 times higher than that of culturing neural stem cells only. Conclusion: These results revealed that some factors secreted into the intercellular microenvironment by adult neuron cells can stimulate the differentiation of neural stem cells, pointing to the importance of developing multicellular culture systems such as the perfused microfluidic system we report here to better resemble the in vivo situation.

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“…Brocadia sp. 40” was enriched from brewery wastewater sludge in a fixed-bed anammox reactor (7). Subsequently, “ Ca.…”

Section: Genome Announcementmentioning

confidence: 99%