Development of a Flow Injection Capillary Chemiluminescent ELISA Using an Imprinted Polymer Instead of the Antibody

Surugiu, Ioana; Švitel, Juraj; Ye, Lei; Haupt, Karsten; Danielsson, Bengt

doi:10.1021/ac0101757

Cited by 83 publications

(35 citation statements)

References 16 publications

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“…The antigen 2,4-dichlorophenoxyacetic acid was coupled to tobacco peroxidase enabling colorimetric (o-PD/H 2 O 2 ) as well as chemiluminescent (luminol/H 2 O 2 ) detection with linear measuring ranges of 40-600 µg·mL −1 and 1-200 µg·mL −1 , respectively. This setup was further developed by coating microtiter plates with imprinted polymer microspheres enabling analyses in a high-throughput format [86], as well as by coating glass capillaries enabling flow-injection competitive assays yielding a dynamic range of 5 pg/mL to 100 ng/mL in a discontinuous detection mode [87].…”

Section: Enzyme-labels In Mip-based Affinity Sensorsmentioning

confidence: 99%

Enzymes as Tools in MIP-Sensors

et al. 2017

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Abstract:Molecularly imprinted polymers (MIPs) have the potential to complement antibodies in bioanalysis, are more stable under harsh conditions, and are potentially cheaper to produce. However, the affinity and especially the selectivity of MIPs are in general lower than those of their biological pendants. Enzymes are useful tools for the preparation of MIPs for both low and high-molecular weight targets: As a green alternative to the well-established methods of chemical polymerization, enzyme-initiated polymerization has been introduced and the removal of protein templates by proteases has been successfully applied. Furthermore, MIPs have been coupled with enzymes in order to enhance the analytical performance of biomimetic sensors: Enzymes have been used in MIP-sensors as "tracers" for the generation and amplification of the measuring signal. In addition, enzymatic pretreatment of an analyte can extend the analyte spectrum and eliminate interferences.

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Section: Enzyme-labels In Mip-based Affinity Sensorsmentioning

confidence: 99%

Enzymes as Tools in MIP-Sensors

et al. 2017

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“…The detection was obtained within 20 min, which is a low assay duration when considering immunochemical assays. The determination of this herbicide has been also carried out substituting the antibody by a molecular imprinted polymer (MIP) (Surugiu 2001) which improves considerably the stability, ease of preparation and cost of the biosensor.…”

Section: Other Pesticidesmentioning

confidence: 99%

Analysis of Pesticides by Flow Injection Coupled with Chemiluminescent Detection: A Review

López-Paz

Catalá-Icardo

2011

Analytical Letters

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The analyses of pesticides carried out by coupling flow injection analysis with chemiluminescence (CL) detection during the last ten years are reported in this review. The review is divided into three sections devoted to the different families of pesticides, namely, organophosphorus, carbamates (together with dithiocarbamates) and other pesticides from the remaining groups. Relevant analytical data such as limits of detection, dynamic ranges, recoveries and sample pretreatments are recorded in three tables. Descriptions about the fundamentals of the CL systems used and new promising approaches as photoinduced chemiluminescence (PICL), multicommutation and molecular connectivity are also included.

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“…By employing a HRPcatalyzed CL reaction using luminol-H2O2-PIP, 33 ng/L of triazine could be sensitively detected. Surugiu et al 31 developed a FICLIA for the detection of the herbicide 2,4-dichlorophenoxyacetic acid by using imprinted polymer that was modified to the inner capillary wall of a glass capillary tube. The target analyte was labeled with the enzyme tobacco peroxidase and, in a competitive format, the bound fraction of the conjugate was quantified by using a photomultiplier tube or a CCD camera.…”

Section: ·4 Enzyme-based CL Techniques 2·4·1 Hrpmentioning

confidence: 99%

Chemiluminescence Platforms in Immunoassay and DNA Analyses

Fan

Cao

et al. 2009

ANAL. SCI.

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IntroductionCL is the production of electromagnetic radiation by a chemical reaction. The CL intensity is directly proportional to the concentration of a limiting reactant involved in the CL reaction. CL has been exploited for its use in a wide range of applications due to its high sensitivity, wide calibration range, and suitability for miniaturization. Protein and DNA analyses have recently attracted much attention. Many different types of techniques, such as electrochemical, optical, radiochemical, and piezoelectronic methods have been applied for protein and DNA analyses. To provide an overview of CL detection assays for DNAs and proteins, we have summarized their essential techniques. Monoplex and multiplex assays are described, and their current reports are intended to provide an overview of the CL techniques currently employed in numerous laboratories. Monoplex AssayA monoplex assay aims to measure the presence of a single analyte in any given sample, in which nanoparticle, DNA enzyme, horseradish peroxidase (HRP), alkaline phosphatase (AP) and acridinium ester are commonly used as labels. 2·1 Nanoparticle-based CL techniquesNanoparticles offer excellent prospects for DNA detection and immunoassay, owing to their many attractive properties. Compared to existing labels, nanoparticles are more stable and cheaper, and easier to be purified. In addition, they indicate faster binding kinetics with high sensitivity and a high reaction rate for many types of monoplex assays, ranging from immunoassays to DNA detection. 2·1·1 Gold nanoparticlesColloidal gold labels are ideal in biotechnological systems for several reasons. Firstly, they can be readily prepared in a wide range of sizes, from approximately 2 nm to above 100 nm. Secondly, the biochemical activity of the labeled biomolecules can be retained when colloidal gold is coupled to the biomolecules and, thirdly, colloidal gold labels can be easily visualized as dense structures within biological entities using transmission electron microscopy. As a biological tag, colloidal gold has been employed in several CL detection systems. 2·1·1·1 Gold nanoparticle-based CL immunoassay. The Au 3+ -luminol reaction, in which 10 -9 M Au 3+ ions can be sensitively detected, is a classic CL reaction. Thousands of Au atoms are contained within the gold nanoparticles; for example, 2.3 ¥ 10 5 Au atoms are theoretically contained in a 20-nm spherical gold particle and, consequently, picomolar detection limits can be attained by employing oxidative gold metal dissolution in acidic solutions. Our group 1,2 reported a sensitive CL immunoassay based on a colloidal gold label after oxidative gold metal dissolution (using 0.01 M HCl-0.5 M NaCl-0.5 mM Br2), using a simple and sensitive luminol-CL reaction (Fig. 1). Reviews Chemiluminescence Platforms in Immunoassay and DNA AnalysesAiping FAN,* Zhijuan CAO,* Huan LI,* Masaaki KAI,** and Jianzhong LU* † *School of Pharmacy, Fudan University, 138 Yixueyuan Road, Shanghai 200032, China ** Faculty of Pharmaceutical Sciences, Graduate Scho...

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Development of a Flow Injection Capillary Chemiluminescent ELISA Using an Imprinted Polymer Instead of the Antibody

Cited by 83 publications

References 16 publications

Enzymes as Tools in MIP-Sensors

Enzymes as Tools in MIP-Sensors

Analysis of Pesticides by Flow Injection Coupled with Chemiluminescent Detection: A Review

Chemiluminescence Platforms in Immunoassay and DNA Analyses

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