Development of a fluorescence assay for highly sensitive detection of <i>Pseudomonas aeruginosa</i> based on an aptamer-carbon dots/graphene oxide system

Wang, Hongying; Chi, Zhe; Cong, Ying; Wang, Zhuangzhuang; Jiang, Fei; Geng, Jiayue; Zhang, Peng; Ju, Peng; Dong, Qi; Liu, Chenguang

doi:10.1039/c8ra04819c

Cited by 51 publications

(22 citation statements)

References 25 publications

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“…F23 laid the foundation for many sensitive and rapid detection methods for P. aeruginosa. Similarly, another specific aptamer against P. aeruginosa (ATCC 27853) with a K d of 15.16 ± 3.62 nM was obtained by other researchers (Wang et al, 2018). Moreover, Soundy and Day (2017) obtained three chimeric DNA aptamers, with K d values in the nanomolar range, against live biofilm-derived PA692 P. aeruginosa cells.…”

Section: P Aeruginosa Aptamers Selected In Recent Yearsmentioning

confidence: 77%

“…Owing to their attractive properties, fluorescent nanomaterials, such as carbon dots (CDs), quantum dots (QDs), and upconversion nanoparticles (UCNPs), have been widely applied in biosensor fabrication ( Chen et al, 2018 ). Wang et al (2018) constructed an aptamer-based fluorescence assay, in which the specific aptamer was conjugated with photoluminescent CDs as the fluorescent probe and graphene oxide (GO) served as the anchor and quencher. The use of GO can reduce the background signals of CDs and improve sensitivity.…”

Section: Aptasensors Based On Optical Transductionmentioning

confidence: 99%

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Recent Advances in Aptamer-Based Biosensors for Detection of Pseudomonas aeruginosa

Zheng

Gao

et al. 2020

Front. Microbiol.

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Increasing concerns about nosocomial infection, food and environmental safety have prompted the development of rapid, accurate, specific and ultrasensitive methods for the early detection of critical pathogens. Pseudomonas aeruginosa is one of the most common pathogens that cause infection. It is ubiquitous in nature, being found in water, soil, and food, and poses a great threat to public health. The conventional detection technologies are either time consuming or readily produce false positive/negative results, which makes them unsuitable for early diagnosis and spot detection of P. aeruginosa. To circumvent these drawbacks, many efforts have been made to develop biosensors using aptamers as bio-recognition elements. Various aptamer-based biosensors for clinical diagnostics, food, and environmental monitoring of P. aeruginosa have been developed in recent years. In this review, we focus on the latest advances in aptamer-based biosensors for detection of P. aeruginosa. Representative biosensors are outlined according to their sensing mechanisms, which include optical, electrochemical and other signal transduction methods. Possible future trends in aptamer biosensors for pathogen detection are also outlined.

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Section: P Aeruginosa Aptamers Selected In Recent Yearsmentioning

confidence: 77%

Section: Aptasensors Based On Optical Transductionmentioning

confidence: 99%

Recent Advances in Aptamer-Based Biosensors for Detection of Pseudomonas aeruginosa

Zheng

Gao

et al. 2020

Front. Microbiol.

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“…For biosensing applications, CDs should (i) recognize analytes through specific interactions via targeting moieties ( e.g ., antibodies, aptamers, and enzymes) and (ii) change their optical properties (or initiate catalysis) through their interactions with analytes, as depicted in Figure . For example, CDs can modulate their PL intensity, colorimetric wavelength, or photocurrent generation to produce optical sensing signals as a transducer. , The changes in the CDs’ PL intensity have been widely utilized in turn off/on, ratiometric or colorimetric sensors to detect analytes ( e.g ., metal ions, − glucose, − cholesterol, , and other chemicals , ). Table summarizes light-mediated sensing applications using CDs.…”

Section: Carbon Dots For Light-mediated Biomedicine and Healthcarementioning

confidence: 99%

“…(e) Fluorescence recovery of Apta@CDs/GO system in the presence of target P. aeruginosa . Reprinted with permission under a Creative Commons Attribution-NonCommercial 3.0 Unported License (CC BY-NC 3.0) from ref . Copyright 2018 The Royal Society of Chemistry.…”

Section: Carbon Dots For Light-mediated Biomedicine and Healthcarementioning

confidence: 99%

Photonic Carbon Dots as an Emerging Nanoagent for Biomedical and Healthcare Applications

2020

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As a class of carbon-based nanomaterials, carbon dots (CDs) have attracted enormous attention because of their tunable optical and physicochemical properties, such as absorptivity and photoluminescence from ultraviolet to near-infrared, high photostability, biocompatibility, and aqueous dispersity. These characteristics make CDs a promising alternative photonic nanoagent to conventional fluorophores in disease diagnosis, treatment, and healthcare managements. This review describes the fundamental photophysical properties of CDs and highlights their recent applications to bioimaging, photomedicine (e.g., photodynamic/photothermal therapies), biosensors, and healthcare devices. We discuss current challenges and future prospects of photonic CDs to give an insight into developing vibrant fields of CD-based biomedicine and healthcare.

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“…A fluorometric aptasensor was fabricated by employing aptamer modified carbon nanoparticle and GO as fluorescent and quencher respectively for the sensitive detection of P. aeruginosa. In the presence of bacteria, the fluorescence signal was increased due to binding aptamer and bacteria that caused distance between the aptamer-carbon nanoparticle and Go, the recorded signal related linearly to 10 1 -10 7 CFUmL -1 concentration of bacteria with a LOD of 9 CFUmL -1 [ 149 ]. An Aptasensor consisting of MNP-Aptamer and UNCP-CDNA was developed to detect, low concentration (58 CFUmL -1 ), E.coli in pork meat sample.…”

Section: Biosensors For Infectious Disease Based On Nanomaterialsmentioning

confidence: 99%

Nanomaterial application in bio/sensors for the detection of infectious diseases

Sheikhzadeh

Beni

Zourob

2021

Talanta

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Infectious diseases are a potential risk for public health and the global economy. Fast and accurate detection of the pathogens that cause these infections is important to avoid the transmission of the diseases. Conventional methods for the detection of these microorganisms are time-consuming, costly, and not applicable for on-site monitoring. Biosensors can provide a fast, reliable, and point of care diagnostic. Nanomaterials, due to their outstanding electrical, chemical, and optical features, have become key players in the area of biosensors. This review will cover different nanomaterials that employed in electrochemical, optical, and instrumental biosensors for infectious disease diagnosis and how these contributed to enhancing the sensitivity and rapidity of the various sensing platforms. Examples of nanomaterial synthesis methods as well as a comprehensive description of their properties are explained. Moreover, when available, comparative data, in the presence and absence of the nanomaterials, have been reported to further highlight how the usage of nanomaterials enhances the performances of the sensor.

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Development of a fluorescence assay for highly sensitive detection of Pseudomonas aeruginosa based on an aptamer-carbon dots/graphene oxide system

Abstract: The fluorescence assay for detecting Pseudomonas aeruginosa was achieved by the aptamer-CDs/GD system which exhibited obvious fluorescence recovery when encountered the cells of P. aeruginosa.

Cited by 51 publications

References 25 publications

Recent Advances in Aptamer-Based Biosensors for Detection of Pseudomonas aeruginosa

Recent Advances in Aptamer-Based Biosensors for Detection of Pseudomonas aeruginosa

Photonic Carbon Dots as an Emerging Nanoagent for Biomedical and Healthcare Applications

Nanomaterial application in bio/sensors for the detection of infectious diseases

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