Development of a Fluorescence Polarization Immunoassay for Polycyclic Aromatic Hydrocarbons

Goryacheva, Irina Yu.; Eremin, Sergei A.; Shutaleva, Ekaterina A.; Suchánek, Miloslav; Knopp, Dietmar

doi:10.1080/00032710701297034

Cited by 19 publications

(7 citation statements)

References 38 publications

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“…Many receptors focus their work on fluorescence-based sensing for glucose biosensors [43][44][45][46], DNA biosensors [47][48][49][50][51][52][53] and environmental monitoring, such as phenols [54][55], polycyclic aromatic hydrocarbons [56][57][58], total trihalomethanes (THMs), and haloacetic acids (HAAs) [59], pesticides [60][61][62][63], and metal ions [64][65][66][67][68][69][70][71][72].…”

Section: Fluorescence-based Pesticide Nano Eco-sensors Using the Lbl mentioning

confidence: 99%

Optical and electrical nano eco-sensors using alternative deposition of charged layer

2010

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This review focuses on layer by layer (LBL) assembly-based nano ecological sensor (hereafter, eco-sensor) for pesticide detection, which is one of the most versatile methods. The effects of pesticides on human health and on the environment (air, water, soil, plants, and animals) are of great concern due to their increasing use. We highlight two of the most popular detecting methods, i.e., fluorescence and electrochemical detection of pesticides on an LBL assembly. Fluorescence materials are of great interest among researchers for their sensitivity and reliable detection, and electrochemical processes allow us to investigate synergistic interactions among film components through charge transfer mechanisms in LBL film at the molecular level. Then, we noted some prospective directions for development of different types of sensing systems.

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Section: Fluorescence-based Pesticide Nano Eco-sensors Using the Lbl mentioning

confidence: 99%

Optical and electrical nano eco-sensors using alternative deposition of charged layer

2010

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“…In recent years, immunoassays have represented a fast, sensitive, inexpensive and field-portable analytical method to complement traditional chromatographic procedures for PAHs [ 1 ]. Some immunoassay techniques, such as enzyme-linked immunosorbent assay (ELISA) [ 2 , 3 ], fluorescence polarization immunoassay [ 4 ], chemiluminescent immunoassay [ 5 ], real-time immuno-polymerase chain reaction assay [ 6 ] and immunosensor [ 7 ], were developed for PAHs analysis. Several ELISA test kits for PAHs have been produced and are commercially available, among which RaPID and Ris C are the most widely studied and used [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

Investigating the Quantitative Structure-Activity Relationships for Antibody Recognition of Two Immunoassays for Polycyclic Aromatic Hydrocarbons by Multiple Regression Methods

Zhang

Gao³

et al. 2012

Sensors

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Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous contaminants found in the environment. Immunoassays represent useful analytical methods to complement traditional analytical procedures for PAHs. Cross-reactivity (CR) is a very useful character to evaluate the extent of cross-reaction of a cross-reactant in immunoreactions and immunoassays. The quantitative relationships between the molecular properties and the CR of PAHs were established by stepwise multiple linear regression, principal component regression and partial least square regression, using the data of two commercial enzyme-linked immunosorbent assay (ELISA) kits. The objective is to find the most important molecular properties that affect the CR, and predict the CR by multiple regression methods. The results show that the physicochemical, electronic and topological properties of the PAH molecules have an integrated effect on the CR properties for the two ELISAs, among which molar solubility (S m ) and valence molecular connectivity index (

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“…Numerous other immunoassay formats are now being developed for the quantitative analysis of PAHs in aqueous environmental samples. Examples include enzyme‐linked immunosorbent assays 18, 19, amperometric biosensors 20, piezoelectric biosensors 21, surface plasmon resonance biosensors 22, 23, and polarized fluorescence biosensors 24.…”

Section: Introductionmentioning

confidence: 99%

Near real‐time, on‐site, quantitative analysis of PAHs in the aqueous environment using an antibody‐based biosensor

Spier

Vadas

Kaattari

et al. 2011

Enviro Toxic and Chemistry

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Rapid, on-site, quantitative assessments of dissolved polycyclic aromatic hydrocarbons (PAHs) were demonstrated for two field applications. The platform, a KinExA Inline Sensor (Sapidyne Instruments), employed the monoclonal anti-PAH antibody, 7B2.3, which has specificity for 3- to 5-ring PAHs. A spatial study was conducted near a dredging site where contaminated sediments were being removed, and a temporal study was performed during a rainfall event. Most importantly, the generation of near real-time data guided management decisions in the field and determined proper sampling protocols for conventional analyses. The method was able to determine PAH concentrations as low as 0.3 µg/L, within 10 min of sample acquisition, and to assess 80+ samples (not including standards and blanks) in less than 3 d. These results were compared with a laboratory-based gas chromatography-mass spectrometry method in which a wide array of PAHs, including alkylated homologs, were examined. This system shows great promise as a field instrument for the rapid monitoring of PAH pollution.

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Development of a Fluorescence Polarization Immunoassay for Polycyclic Aromatic Hydrocarbons

Cited by 19 publications

References 38 publications

Optical and electrical nano eco-sensors using alternative deposition of charged layer

Optical and electrical nano eco-sensors using alternative deposition of charged layer

Investigating the Quantitative Structure-Activity Relationships for Antibody Recognition of Two Immunoassays for Polycyclic Aromatic Hydrocarbons by Multiple Regression Methods

Near real‐time, on‐site, quantitative analysis of PAHs in the aqueous environment using an antibody‐based biosensor

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