Development of a gene-deleted live attenuated candidate vaccine against fish virus (ISKNV) with low pathogenicity and high protection

Zeng, Ruoyun; Pan, Weiqiang; Lin, Yifan; Luo, Zhiyong; Li, Zhimin; Weng, Shaoping; He, Jianguo; Guo, Chang-Jun

doi:10.1016/j.isci.2021.102750

Cited by 21 publications

(11 citation statements)

References 31 publications

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“…The MOI was calculated by the number of virions/the number of cells in the plate well. [ 31 ]. The number of virions was calculated by the number of ISKNV genome copies using the standard-curve method of absolute quantitative real-time PCR (qPCR).…”

Section: Methodsmentioning

confidence: 99%

Evidence for a Novel Antiviral Mechanism of Teleost Fish: Serum-Derived Exosomes Inhibit Virus Replication through Incorporating Mx1 Protein

Chen

Wang

et al. 2021

IJMS

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Exosomes are associated with cancer progression, pregnancy, cardiovascular diseases, central nervous system-related diseases, immune responses and viral pathogenicity. However, study on the role of exosomes in the immune response of teleost fish, especially antiviral immunity, is limited. Herein, serum-derived exosomes from mandarin fish were used to investigate the antiviral effect on the exosomes of teleost fish. Exosomes isolated from mandarin fish serum by ultra-centrifugation were internalized by mandarin fish fry cells and were able to inhibit Infectious spleen and kidney necrosis virus (ISKNV) infection. To further investigate the underlying mechanisms of exosomes in inhibiting ISKNV infection, the protein composition of serum-derived exosomes was analyzed by mass spectrometry. It was found that myxovirus resistance 1 (Mx1) was incorporated by exosomes. Furthermore, the mandarin fish Mx1 protein was proven to be transferred into the recipient cells though exosomes. Our results showed that the serum-derived exosomes from mandarin fish could inhibit ISKNV replication, which suggested an underlying mechanism of the exosome antivirus in that it incorporates Mx1 protein and delivery into recipient cells. This study provided evidence for the important antiviral role of exosomes in the immune system of teleost fish.

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Section: Methodsmentioning

confidence: 99%

Evidence for a Novel Antiviral Mechanism of Teleost Fish: Serum-Derived Exosomes Inhibit Virus Replication through Incorporating Mx1 Protein

Chen

Wang

et al. 2021

IJMS

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“…Recently, Zeng et al ( 2021 ) reported the result of a study on a live attenuated gene-deleted vaccine candidate, ΔORF022L, in mandarin fish fry-1 (MFF-1) cells against infectious spleen and kidney necrosis virus (ISKNV). One hundred percent survival of the ΔORF022L-infected fish challenged with ISKNV infection was reported.…”

Section: Types Of Vaccines For Fish Pathogens In Aquaculture and Thei...mentioning

confidence: 99%

A review on the recent advances and application of vaccines against fish pathogens in aquaculture

2022

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Globally, aquaculture has faced serious economic problems due to bacterial, viral, and various other infectious diseases of different origins. Even though such diseases are being detected and simultaneously treated with several therapeutic and prophylactic methods, the broad-spectrum activity of vaccines plays a vital role as a preventive measure in aquaculture. However, treatments like use of antibiotics and probiotics seem to be less effective when new mutant strains develop and disease causing pathogens become resistant to commonly used antibiotics. Therefore, vaccines developed by using recent advanced molecular techniques can be considered as an effective way of treating disease causing pathogens in aquatic organisms. The present review emphasizes on the current advances in technology and future outlook with reference to different types of vaccines used in the aquaculture industries. Beginning with traditional killed/inactivated and live attenuated vaccines, this work culminates in the review of modern new generation ones including recombinant, synthetic peptides, mucosal and DNA, subunit, nanoparticle-based and plant-based edible vaccines, reverse vaccinology, and monovalent and polyvalent vaccines.

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“…The RT-qPCR reactions were performed with a SYBR premix ExTaqTM (Takara, Tokyo, Japan) on a LightCycler ® 480 instrument (Roche Diagnostics, Switzerland) as previously described [ 20 ]. The primers for RT-qPCR were designed using Primer premier software v5.0 program (Applied Biosystems, Table 2 ).…”

Section: Methodsmentioning

confidence: 99%

“…Then, the 96-well dishes were incubated at 27 °C. After 7 days of incubation, the number of positive and negative wells were recorded; the TCID 50 was calculated using the Spearman–Karber method as previously described [ 20 ].…”

Section: Methodsmentioning

confidence: 99%

The Interaction of Mandarin Fish DDX41 with STING Evokes type I Interferon Responses Inhibiting Ranavirus Replication

Qin

Luo

Pan

et al. 2022

Viruses

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DDX41 is an intracellular DNA sensor that evokes type I interferon (IFN-I) production via the adaptor stimulator of interferon gene (STING), triggering innate immune responses against viral infection. However, the regulatory mechanism of the DDX41-STING pathway in teleost fish remains unclear. The mandarin fish (Siniperca chuatsi) is a cultured freshwater fish species that is popular in China because of its high market value. With the development of a high-density cultural mode in mandarin fish, viral diseases have increased and seriously restricted the development of aquaculture, such as ranavirus and rhabdovirus. Herein, the role of mandarin fish DDX41 (scDDX41) and its DEAD and HELIC domains in the antiviral innate immune response were investigated. The level of scDDX41 expression was up-regulated following treatment with poly(dA:dT) or Mandarin fish ranavirus (MRV), suggesting that scDDX41 might be involved in fish innate immunity. The overexpression of scDDX41 significantly increased the expression levels of IFN-I, ISGs, and pro-inflammatory cytokine genes. Co-immunoprecipitation and pull-down assays showed that the DEAD domain of scDDX41 recognized the IFN stimulatory DNA and interacted with STING to activate IFN-I signaling pathway. Interestingly, the HELIC domain of scDDX41 could directly interact with the N-terminal of STING to induce the expression levels of IFN-I and ISGs genes. Furthermore, the scDDX41 could enhance the scSTING-induced IFN-I immune response and significantly inhibit MRV replication. Our work would be beneficial to understand the roles of teleost fish DDX41 in the antiviral innate immune response.

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Development of a gene-deleted live attenuated candidate vaccine against fish virus (ISKNV) with low pathogenicity and high protection

Cited by 21 publications

References 31 publications

Evidence for a Novel Antiviral Mechanism of Teleost Fish: Serum-Derived Exosomes Inhibit Virus Replication through Incorporating Mx1 Protein

Evidence for a Novel Antiviral Mechanism of Teleost Fish: Serum-Derived Exosomes Inhibit Virus Replication through Incorporating Mx1 Protein

A review on the recent advances and application of vaccines against fish pathogens in aquaculture

The Interaction of Mandarin Fish DDX41 with STING Evokes type I Interferon Responses Inhibiting Ranavirus Replication

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