Development of a High-Throughput DNA Fiber Analysis Device Using MEMS Technology

Suzuki, Takao; Terao, Kyohei; Suzuki, Hiroyuki; Nitta, Yumiko; Takao, Hidekuni; Shimokawa, Fusao; Oohira, Fumikazu; Hiramaru, Daisuke; Kotera, Hidetoshi

doi:10.1541/ieejsmas.133.139

Cited by 2 publications

(4 citation statements)

References 13 publications

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“…For fabricating the well mold with inclined structures, SU‐8 is processed by rotated/inclined exposure method that supports batch processing of 3D microstructures with a single mask. With this method, a substrate with applied resist is placed on an inclined rotary stage, and exposure is performed from the substrate backside while rotating the stage.…”

Section: Fabrication Of Micro‐inclined Well Arraymentioning

confidence: 99%

“…In this context, we propose a micro‐inclined well array with tapered walls for effective cell immobilization. As regards fabrication of inclined structures, we fabricated well arrays by PDMS molding into molds produced by rotated/inclined exposure method . We carried out bead immobilization experiments with prototype arrays to estimate residual rate of immobilized beads after liquid exchange.…”

Section: Introductionmentioning

confidence: 99%

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Development of micro‐inclined well array for trapping single cells

Tsukamoto

Furuya

Shimagami

et al. 2018

Elect Comm in Japan

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We investigated the shape of a well to trap single cells for the purpose of improving the cell trapping rate after cell seeding and liquid exchange in a cell microarray. The wells having vertical and inclined tapered side‐wall were made using three‐dimensional UV photolithography. Changes in cell trapping rate by the shape of the wells were evaluated by trapping tests with fluorescent microbeads and living cells. As a result of trapping test with living cells, bead residual rate of the microarray with inclined tapered wells is up to 2.9 times higher than that with vertical wells.

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Section: Fabrication Of Micro‐inclined Well Arraymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Development of micro‐inclined well array for trapping single cells

Tsukamoto

Furuya

Shimagami

et al. 2018

Elect Comm in Japan

Self Cite

View full text Add to dashboard Cite

show abstract

“…For fabricating the well mold with inclined structures, SU-8 is processed by rotated/inclined exposure method 15,16 that supports batch processing of 3D microstructures with a single mask. With this method, a substrate with applied resist is placed on an inclined rotary stage, and exposure is performed from the substrate backside while rotating the stage.…”

Section: Fabrication Of Micro-inclined Well Arraymentioning

confidence: 99%

“…As regards fabrication of inclined structures, we fabricated well arrays by PDMS molding into molds produced by rotated/inclined exposure method. 15,16 We carried out bead immobilization experiments with prototype arrays to estimate residual rate of immobilized beads after liquid exchange. In addition, we carried out cell immobilization experiments to compare and evaluate well arrays with conventional vertical wells and inverse-tapered wells as proposed in this study.…”

Section: Introductionmentioning

confidence: 99%

Development of Micro Inclined Well Array for Trapping Single Cells

et al. 2018

Self Cite

View full text Add to dashboard Cite

We investigated the shape of a well to trap single cells for the purpose of improving the cell trapping rate after cell seeding and liquid exchange in a cell microarray. The wells having vertical and inclined tapered side-wall were made using three-dimensional UV photolithography. Changes in cell trapping rate by the shape of the wells were evaluated by trapping tests with fluorescent microbeads and living cells. As a result of trapping test with living cells, bead residual rate of the microarray with inclined tapered wells is up to 2.9 times higher than that with vertical wells. K E Y W O R D S3D photolithography, single cell, well array Electron Comm Jpn. 2018;101:45-50.

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Development of a High-Throughput DNA Fiber Analysis Device Using MEMS Technology

Cited by 2 publications

References 13 publications

Development of micro‐inclined well array for trapping single cells

Development of micro‐inclined well array for trapping single cells

Development of Micro Inclined Well Array for Trapping Single Cells

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