Development of a highly efficient indigo dyeing method using indican with an immobilized β-glucosidase from Aspergillus niger

Song, Jingyuan; Imanaka, Hiroyuki; Imamura, Koreyoshi; Kajitani, Kouichi; Nakanishi, Koji

doi:10.1016/j.jbiosc.2010.03.010

Cited by 34 publications

(21 citation statements)

References 23 publications

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“…In a typical experiment, a 0.1-mL portion of the enzyme solution (1.0 mg/mL) was injected onto a Superose 6HR column (1 Â 30 cm) and a Superose 12HR column (1 Â 30 cm) connected in series, and eluted at a flow rate of 0.4 mL/min with buffer D, with monitoring at 280 nm. Pyranose oxidase (SigmaAldrich), alcohol dehydrogenase (Sigma-Aldrich), Aspergillus niger -glucosidase, 51) and the High Molecular Weight (HMW) and Low Molecular Weight (LMW) gel filtration calibration kits (GE Healthcare) were used for calibration.…”

Section: Materials Biolactamentioning

confidence: 99%

Causes of the Production of Multiple Forms of β-Galactosidase byBacillus circulans

Song

Abe

Imanaka

et al. 2011

Bioscience, Biotechnology, and Biochemistry

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Section: Materials Biolactamentioning

confidence: 99%

Causes of the Production of Multiple Forms of β-Galactosidase byBacillus circulans

Song

Abe

Imanaka

et al. 2011

Bioscience, Biotechnology, and Biochemistry

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“…Indigo is a dark blue crystalline powder and one of the oldest blue dyes that has been known for more than 4000 years. It can be obtained from a variety of plant sources such as Indigofera tinctoria (Africa, Asia, East India, South America), Polygonum tinctorium (China, Korea) and Isatistinctoria (Europe) [15]. It is commonly used to dye cotton yarn for the production of denim cloth to make blue jeans [5,18].…”

Section: Introductionmentioning

confidence: 99%

“…The latter is then converted to indigo through several steps. Also, the extraction needs a strong base such as lye and other substances that are harmful to the environment [15,19]. Since the natural source for indigo is limited, chemical synthesis becomes a more economic method to produce this dye.…”

Section: Introductionmentioning

confidence: 99%

Efficient production of indigoidine in Escherichia coli

Gage

Zhan

2015

Journal of Industrial Microbiology and Biotechnology

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Indigoidine is a bacterial natural product with antioxidant and antimicrobial activities. Its bright blue color resembles the industrial dye indigo, thus representing a new natural blue dye that may find uses in industry. In our previous study, an indigoidine synthetase Sc-IndC and an associated helper protein Sc-IndB were identified from Streptomyces chromofuscus ATCC 49982 and successfully expressed in Escherichia coli BAP1 to produce the blue pigment at 3.93 g/l. To further improve the production of indigoidine, in this work, the direct biosynthetic precursor L-glutamine was fed into the fermentation broth of the engineered E. coli strain harboring Sc-IndC and Sc-IndB. The highest titer of indigoidine reached 8.81 ± 0.21 g/l at 1.46 g/l L-glutamine. Given the relatively high price of L-glutamine, a metabolic engineering technique was used to directly enhance the in situ supply of this precursor. A glutamine synthetase gene (glnA) was amplified from E. coli and co-expressed with Sc-indC and Sc-indB in E. coli BAP1, leading to the production of indigoidine at 5.75 ± 0.09 g/l. Because a nitrogen source is required for amino acid biosynthesis, we then tested the effect of different nitrogen-containing salts on the supply of L-glutamine and subsequent indigoidine production. Among the four tested salts including (NH4)2SO4, NH4Cl, (NH4)2HPO4 and KNO3, (NH4)2HPO4 showed the best effect on improving the titer of indigoidine. Different concentrations of (NH4)2HPO4 were added to the fermentation broths of E. coli BAP1/Sc-IndC+Sc-IndB+GlnA, and the titer reached the highest (7.08 ± 0.11 g/l) at 2.5 mM (NH4)2HPO4. This work provides two efficient methods for the production of this promising blue pigment in E. coli.

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“…However, the use of synthetic indigo dye has been preferred to natural indigo and is now widely used to dye cotton fabrics, especially work clothes and blue jeans (Song et al 2010). On the other hand, indigo dye is still consumed as the highest pigment among the industrial chemicals in the world annually (Tansı 1998;Blackburn et al 2009;Osimani et al 2012).…”

Section: Introductionmentioning

confidence: 99%

High yielding indigo sources in native Isatis (Brassicaceae) taxa from Turkey

Karaman

Dıraz

Çömlekçioğlu

et al. 2015

Genet Resour Crop Evol

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Indigo dye has a distinctive blue color and is one of the oldest known dyestuffs. In order to identify new Isatis genotypes for indigo production, 14 Isatis taxa collected from native area were grown under field conditions in Kahramanmaraş, Turkey. The effects of taxa and harvest times on leaf, indigo and indican yield were analyzed and compared with culture species (Isatis tinctoria and Isatis indigotica) by using spectrophotometer and HPLC. High indigo yield (87.13 kg/ha) was obtained from Isatis constricta and I. tinctoria subsp. tomentolla (Mersin location) had high indican yield (12.25 mg/g) in fresh leaves. The present work identified high indigo yielding taxa that may be used for genetic improvement in order to re-introduce Isatis species in the agricultural systems of Mediterranean regions. Especially I. constricta can be evaluated in terms of higher indigo yield than the native and culture taxa. I. constricta is an endemic plant in endangered category. This situation reveals that the need for the conservation of the species. To the best of our knowledge, there is no previous study reported about indigo contents of the studied 11 Isatis spp. so far.

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Development of a highly efficient indigo dyeing method using indican with an immobilized β-glucosidase from Aspergillus niger

Cited by 34 publications

References 23 publications

Causes of the Production of Multiple Forms of β-Galactosidase byBacillus circulans

Causes of the Production of Multiple Forms of β-Galactosidase byBacillus circulans

Efficient production of indigoidine in Escherichia coli

High yielding indigo sources in native Isatis (Brassicaceae) taxa from Turkey

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